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2 protocols using octadecyltrimethyl ammonium bromide

1

Synthesis of Metal Nanoparticles

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Chloroauric acid hydrate (HAuCl4·4H2O, analytical grade), l-ascorbic acid (AA, analytical grade), sodium bromide (NaBr, analytical grade), cetyltrimethyl ammonium bromide (CTAB, analytical grade) and octadecyltrimethyl ammonium bromide (OTAB, analytical grade) were all purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Palladium(ii) chloride (PdCl2, analytical grade) was provided by Shanghai Fine Chemical Materials Institute. Phosphate buffer solution (PBS) was prepared with tablets obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). 30% w/w hydrogen peroxide (H2O2, analytical grade) was received from Guangzhou Jinhuada Chemical Reagent Co. Ltd. H2PdCl4 solution was prepared by dissolving PdCl2 in HCl solution followed by dilution with ultrapure water. All reagents were used as received without further purification. All aqueous solutions were prepared with ultrapure water with a resistivity of 18.2 MΩ cm−1.
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2

Synthesis and Characterization of Polyoxometalate Compounds

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Sodium tungstate dihydrate (Na2WO4·2H2O), Sodium phosphate dibasic (Na2HPO4), Iron nitrate nonahydrate (Fe(NO3)3·9H2O), Sodium bicarbonate (NaHCO3) and octadecyltrimethylammonium bromide (ODAB) were acquired from Sinopharm Chemical Reagent Co. Ltd., China. Cisplatin and carboplatin were purchased from Nanjing Jingzhu Bio-technology Co., Ltd. (Nanjing, China). Trypsin, phosphate buffer saline (PBS), dimethyl sulfoxide (DMSO) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma (St. Louis, MO, United States). Fetal bovine serum (FBS), RPMI-1640 Medium and penicillin-streptomycin were purchased from Gibco BRL (Grand Island, NY, United States). Annexin V-FITC apoptosis detection kit and cell cycle kit were obtained from BD Biosciences (San Jose, CA, United States). All chemicals and solvents were used as received from commercial sources without further purification. (TEA)10[(PW11FeO39)2O]·3H2O and (TMA)10[(PW11FeO39)2O]·4H2O (TEA = tetraethyl ammonium bromide, TMA = tetramethyl ammonium bromide) were prepared according to the literature (Pichon et al., 2008 (link)), and the characteristic data were consistent with previously published values (Pichon et al., 2008 (link); Santos et al., 2012 (link)).
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