HeLa TZM-bl indicator cells were seeded in 96-well plates in duplicates (4000 cells/well) and infected the next day with 1 ng VSV-G-pseudotyped virions standardized for p24 content by ELISA (Aalto Bio Reagents, Dublin, Ireland). After overnight incubation, fresh medium was added. 48 h later, cells were washed with PBS and infection was detected using the Gal-Screen chemiluminescent reporter gene assay system (Applied Biosystems, Waltham, MA, USA). β-Galactosidase activity was quantified as relative light units per second using a Synergy HT microplate reader and Gen5 Data Analysis Software (BioTek, Winooski, VT, USA).
Gal screen chemiluminescent reporter gene assay system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Gal-Screen chemiluminescent reporter gene assay system is a laboratory equipment used for the detection and quantification of gene expression. It utilizes a chemiluminescent signal to measure the activity of the reporter gene, providing a sensitive and reliable method for analyzing gene regulation and expression in various experimental settings.
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Lab products found in correlation
2 protocols using gal screen chemiluminescent reporter gene assay system
1
VSV-G Pseudotyped Virus Infection Assay
2
VSV-G Pseudotyped Virus Infection Assay
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HeLa TZM-bl indicator cells were seeded in 96-well plates in duplicates (4000 cells/well) and infected the next day with 1 ng vesicular stomatitis virus-glycoprotein (VSV-G)-pseudotyped virions standardized for p24 content by ELISA (Aalto Bio Reagents). After overnight incubation, fresh medium was added. 48 h later, cells were washed with PBS and infection was detected using the Gal-Screen chemiluminescent reporter gene assay system (Applied Biosystems, Waltham, MA, USA). β-Galactosidase activity was quantified as relative light units per second using a Synergy HT microplate reader and Gen5 Data Analysis Software (BioTek, Winooski, VT, USA).
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