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Anti cyclin a

Manufactured by Merck Group
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Anti-Cyclin A is a laboratory equipment product used for the detection and measurement of cyclin A, a protein involved in cell cycle regulation. It is a specific antibody that binds to cyclin A, allowing for its identification and quantification in biological samples.

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Lab products found in correlation

Anti γ tubulin, by Merck Group (3 mentions) Anti cdk2 phospho thr160, by Cell Signaling Technology (3 mentions) Anti cyclin e, by Santa Cruz Biotechnology (2 mentions) Anti lc3a b, by Cell Signaling Technology (2 mentions) Anti cdk2, by Merck Group (2 mentions) Anti h2ax phospho ser139, by Abcam (2 mentions) Anti cyclin e, by Cell Signaling Technology (2 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Ecl western blotting reagent, by GE Healthcare (1 mentions) Pvdf transfer membrane, by PerkinElmer (1 mentions) Pierce 660 nm protein assay, by Thermo Fisher Scientific (1 mentions) Anti calretinin, by Merck Group (1 mentions) Anti nrf1, by Santa Cruz Biotechnology (1 mentions) Mouse anti actin, by MP Biomedicals (1 mentions) Super rx fuji x ray film, by Fujifilm (1 mentions) Anti actin antibody, by Santa Cruz Biotechnology (1 mentions) Anti irf1 sc 497, by Santa Cruz Biotechnology (1 mentions) Anti e2f1, by Merck Group (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Merck Group (1 mentions)

Spelling variants of this product

Cyclin A (12 citations) Mouse anti-Cyclin A (2 citations) Anti-CYCLIN A antibody (2 citations)

8 protocols using anti cyclin a

1

Western Blot Analysis of Protein Extracts

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Total protein extracts were prepared by lysing the cells with hot Laemmli sample buffer (60 mM Tris-Cl pH 6.8, 100 mM DTT, 5% glycerol, 1, 7% SDS) and pressed few times through syringes (26 G). Protein concentration was determined using a Pierce™ 660nm Protein Assay (Thermo Scientific). A total of 5 μg protein per extract was separated on denaturing 10–20% gradient SDS-PAGE gels. Proteins were transferred on PVDF transfer membranes (0.45 μm, Perkin Elmer). For Western blotting, membranes were probed with the following primary antibodies: anti-calretinin (Sigma, HPA007306), anti-cyclin E (Santa Cruz Sc-247), anti-cyclin A (Millipore, 06–138), anti-NRF-1 (Santa Cruz SC33771, H300) and mouse anti-actin (#69100) from MP Biomedicals. Membranes were then incubated with the secondary antibody rabbit anti-mouse IgG-HRP (A-5420) from Antell, and goat anti-rabbit IgG-HRP (#7074) from Cell Signaling. The signals were detected by enhanced chemiluminescence (ECL™ Western Blotting Reagents, GE Healthcare) and detected on photosensitive film (Super RX Fuji x-Ray Film, Fujifilm). Relative quantification was assessed using ImageJ (NIH).
Kresoja-Rakic J., Kapaklikaya E., Ziltener G., Dalcher D., Santoro R., Christensen B.C., Johnson K.C., Schwaller B., Weder W., Stahel R.A, & Felley-Bosco E. (2016). Identification of cis- and trans-acting elements regulating calretinin expression in mesothelioma cells. Oncotarget, 7(16), 21272-21286.
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2

IRF-1 Immunoprecipitation and Western Blot Analysis

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WCE from MCF7, MCF7/dnIRF-1, MCF7/control, or MCF10A cells were prepared and subjected to Western Blot analysis or immunoprecipitation as previously described [37] . Briefly, 300 μg of WCE were incubated with 1 μg of polyclonal anti-IRF-1 antibody (sc-13041 Santa Cruz Biotechnology Inc., Santa Cruz, CA.) overnight at 4 °C and then Ultralink immobilized protein A/G-Sepharose (Pierce Biotechnology, Rockford, IL) was added for 2 h at room temperature. After extensive washing, immunoprecipitates were eluted by boiling the beads for 3 min in SDS sample buffer and then subjected to Western Blot analysis. IRF-1 and IRF-1 mutated form (IRF-1 3A) were detected by anti-IRF-1 (sc-497 Santa Cruz Biotechnology) antibody; anti-UbK48 Apu2, anti-E2F1 and anti-CyclinA were from Millipore; anti-IKK-ε was from Active Motive; anti-phospho-IKK epsilon (Ser172) antibody were from Cell Signaling Technology; anti-p21, anti-PCNA were from Santa Cruz Biotechnology. Levels of IRF-1, p21, E2F1, Cyclin A and PCNA proteins, relative to levels of endogenous actin protein were quantified using UVP Vision Works LS Image Acquisition software. Anti-actin antibody (Santa Cruz Biotechnology) was used in each experiment as protein loading control; the secondary antibody was from Calbiochem.
Remoli A.L., Sgarbanti M., Perrotti E., Acchioni M., Orsatti R., Acchioni C., Battistini A., Clarke R, & Marsili G. (2020). IκB kinase-ε-mediated phosphorylation triggers IRF-1 degradation in breast cancer cells. Neoplasia (New York, N.Y.), 22(10), 459-469.
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3

Western Blot Analysis of Islet Cell Proteins

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For westerns, isolated islets or INS-1 cells grown in six well plates were homogenized in RIPA buffer and resolved on SDS-PAGE. Primary antibodies used were rabbit anti-c-Myc, 1:1000 (#5605, Cell Signaling, Boston, MA) and mouse anti-Gapdh, 1:5000 (#sc-32233, Santa Cruz). Secondary antibodies were anti-rabbit IRDye 800CW (#827-08365, Odyssey) and anti-mouse IRDye 680LT (#827-11080, Odyssey). Western analysis for cell cycle proteins on islets isolated from mice was carried out using the following antibodies—anti-Cdk1, 1:1000 (#9112, Cell Signaling Technologies), anti-Cdk2, 1:500 (#163, Santa Cruz Biotechnology), anti-Cdk4, 1:1000 (#260, Santa Cruz Biotechnology), anti-Cdk6, 1:500 (#3126, Abcam Inc.), anti-Cyclin A, 1:500 (#4710, Sigma), anti-Cyclin D3, 1:500 (#28283, Abcam Inc.), anti-Cyclin E, 1:500 (#481, Santa Cruz Biotechnology), anti-tubulin, 1:2000 (Calbiochem) and anti-actin, 1:2000 (Sigma).7 (link).
Puri S., Roy N., Russ H.A., Leonhardt L., French E.K., Roy R., Bengtsson H., Scott D.K., Stewart A.F, & Hebrok M. (2018). Replication confers β cell immaturity. Nature Communications, 9, 485.
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4

Antibody Panel for Cell Signaling

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The following antibodies were obtained commercially: anti-γ-tubulin (T6557), anti-cyclin A (C4710), and anti-α-tubulin (T9026) (Sigma, St. Louis, MO), anti-CDK2 (#2546), anti-CDK2 phospho-Thr160 (#2561), anti-Cleaved Caspase-3 (Asp175) (#9661), anti-CYP11A1 (D8F4F) (#14217), anti-Ad4BP/SF-1 (STF-1, D1Z2A) (#12800), and anti-LC3A/B (#12741) (Cell Signaling, Beverly, MA), anti-H2AX phospho-Ser139 (ab2893, Abcam, Cambridge, UK), anti- Beclin 1/ATG6 (NB500-249) (Novus, Littleton, CO), anti-cyclin E1 (HE-12, GTX23927), anti-actin (GTX109639) and anti-GAPDH (GTX100118) (Genetex, Irvine, CA).
Syu J.S., Baba T., Huang J.Y., Ogawa H., Hsieh C.H., Hu J.X., Chen T.Y., Lin T.C., Tsuchiya M., Morohashi K.I., Huang B.M., Lu F.L, & Wang C.Y. (2017). Lysosomal activity maintains glycolysis and cyclin E1 expression by mediating Ad4BP/SF-1 stability for proper steroidogenic cell growth. Scientific Reports, 7, 240.
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5

Characterization of Cellular Proteins

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The following antibodies were obtained commercially: anti-γ-tubulin, polyclonal anti-FLAG, anti-Cyclin A, monoclonal anti-FLAG M2, anti-α-tubulin and anti-acetylated-α-tubulin (all from Sigma, St. Louis, MO), anti-Cyclin E, anti-CDK2 phospho-Thr160, anti-Akt and anti-Akt phospho-Thr308 (Cell Signaling, Beverly, MA), anti-centrin 20H5 (Millipore, Billerica, MA), polyclonal anti-β-catenin and anti-GSK3β (Abcam, Cambridge, UK), anti-Ku70 (Genetex, Trvine, CA), anti-DNA-PKcs, and anti-DNA-PKcs phospho-Thr 2609 (Santa Cruz Biotech, Santa Cruz, CA). The immune sera against SF-1 have been described previously [19 (link)].
Wang C.Y., Lai P.Y., Chen T.Y, & Chung B.C. (2014). NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and β-catenin accumulation. Cell Communication and Signaling : CCS, 12, 55.
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6

NSCLC Cell Line A549 Culture Protocol

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NSCLC cell line A549 (Shanghai Institute of Cell Biology, China) was cultured in Roswell Park Memorial Institute (RPMI) Medium 1640 containing 10% fetal bovine serum (FBS, Gibco Corporation, Carlsbad, CA, USA), 1 mM glutamine, 1.5 g/L sodium bicarbonate, and 1% penicillin/streptomycin. Primary antibodies included anti-B-cell lymphoma-2 (Bcl-2), anti-BCL2-Associated X (Bax), anti-poly ADP ribose polymerase (PARP)-1, anti-myeloid cell leukemia 1 (Mcl-1), anti-Bcl-xL, anti-caspase-3, anti-caspase-8, anti-caspase-9 anti-PARP, anti-AMPK, anti-p-AMPK, anti-Akt, anti-p-Akt, anti-c-Jun, Phospho-c-jun (p-c-Jun), anti-c-Jun NH2-Terminal Kinase (JNK), anti-uncoordinated 51-like kinase 1 (ULK1), anti-p-JNK, anti-mTOR, anti-Beclin-1, anti-light chain 3 (LC3), anti-sequestosome 1 (p62) and anti-GAPDH, anti-DAPI, and anti-Tom20 were purchased from Cell Signaling Technology Inc (Beverly, MA, USA). Anti-cyclin A, anti-cyclin B2, anti-cyclin D1, anti-cyclin D3, anti-p21, and anti-Cdc2 were purchased from Sigma-Aldrich (Munich, Germany), as was analytical-grade NCTD.
Liu Z., Li B., Cao M, & Jiang J. (2021). Norcantharidin triggers apoptotic cell death in non-small cell lung cancer via a mitophagy-mediated autophagy pathway. Annals of Translational Medicine, 9(12), 971.
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7

Immunoblotting for Cell Cycle Markers

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The following antibodies were obtained commercially: anti-γ-tubulin (T6557), anti-Cyclin A (C4710) and anti-α-tubulin (T9026) (Sigma), anti-CDK2 (#2546), anti-Chk2 (#6334), anti-Chk2 phospho-Thr68 (#2661), anti-ERK1/2 (#4695), anti-phospho-ERK1/2 on Thr202/Tyr204 (#4370), anti-Cyclin E (#4132), anti-CDK2 phospho-Thr160 (#2561) and anti-LC3A/B (#12741) (Cell Signaling, Beverly, MA, USA), anti-H2AX phospho-Ser139 (ab2893, Abcam, Cambridge, UK), anti-actin (GTX109639) and anti-GAPDH (GTX100118) (Genetex, Irvine, CA, USA).
Chen T.Y., Syu J.S., Lin T.C., Cheng H.L., Lu F.L, & Wang C.Y. (2015). Chloroquine alleviates etoposide-induced centrosome amplification by inhibiting CDK2 in adrenocortical tumor cells. Oncogenesis, 4(12), e180-.
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8

Comprehensive Immunoblotting Assay Protocol

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Immunoblotting was performed as previously described 19 . The following antibodies were used for immunoblotting: anti-beta-Actin (Sigma, A5441, 1:5000), anti-Cyclin A (Sigma, C4170, 1:1000), anti-Involucrin (Sigma, I9018, 1:1000), anti-p16 (H-156, Santa Cruz, sc-759, 1:500) and (G175-1239, BD Pharmingen, 554079, 1:500), anti-p21 (F-5, Santa Cruz, sc-6246, 1:500), anti-p53 (DO-1, Sigma, P6874, 1:1000), anti-C/EBPβ (C-19, Santa Cruz, sc-150, 1:500), anti-c-H-Ras (F235-1.7.1, Calbiochem, OP23, 1:500), anti-E1A (Santa Cruz, sc-430, 1:2000), anti-HMGA1 (Active Motif, 39615, 1:2000). The anti-Pan-LCE2 antibody (1:1000) was raised in rabbits against a synthetic peptide (RPRLFHRRRHQSPD), as previously described (note, this antigen sequence perfectly matches human LCE2B, C and D, and differs by one amino acid for LCE2A) 26 . The following antibodies were used for ChIP: anti-H3K9me3 (clone
Tomimatsu K., Bihary D., Olan I., Parry A.J., Schoenfelder S., Chan A.S.L., Slater G.S.C., Ito Y., Rugg-Gunn P.J., Kirschner K., Bermejo-Rodriguez C., Seko T., Kugoh H., Shiraishi K., Sayama K., Kimura H., Fraser P., Narita M., Samarajiwa S.A, & Narita M. (2022). Locus-specific induction of gene expression from heterochromatin loci during cellular senescence. Nature aging, 2(1).
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