The ability to regulate blood glucose was measured using an IP glucose tolerance test. Mice were fasted for at least 3 hours prior to testing and weighed at the start of each test session. At the start of the test session, 2 mm of the tail tip was removed using a scalpel blade. The first drop of blood was discarded and the second drop was placed onto a OneTouch Ultra test strip (LifeScan, Inc.) that was inserted into a OneTouch UltraMini Blood Glucose Monitoring System (LifeScan, Inc.) glucose meter to record baseline glucose levels in mg/dL. Additional blood samples were collected from the tail at 15, 30, 60, 90, and 120 minutes following an IP injection of D-(+)-glucose solution (45%, Sigma Life Science) in a volume of 0.01ml/g of body weight and additional blood glucose levels were obtained as described above.
Onetouch ultramini blood glucose monitoring system
Manufactured by LifeScan
Sourced in Canada, United States
The OneTouch UltraMini Blood Glucose Monitoring System is a laboratory equipment product that measures blood glucose levels. It provides users with accurate and reliable results to monitor their blood sugar levels.
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Lab products found in correlation
6 protocols using onetouch ultramini blood glucose monitoring system
1
Intraperitoneal Glucose Tolerance Test
2
Insulin-Induced Glucose Response
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Following 5 hr fasting, mice were injected intraperitoneally with 1.0 U/kg insulin (Sigma), and glucose levels were measured from tail blood before at 15, 30, 60, or 120 min by using the ONETOUCH UltraMini blood glucose monitoring system (LifeScan).
3
Glucose Tolerance in Diabetic Mice
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Overnight fasted db/db and DIO mice were oral gavaged with 1 g/kg glucose, and glucose were measured from tail blood before and 15, 30, 60, or 120 min by using the ONETOUCH UltraMini blood glucose monitoring system (LifeScan).
4
Plasma Glucose and Insulin Measurement
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Blood from all animals in a fed state was collected between 15:00 and 16:00 h by saphenous vein bleeding and used to determine plasma glucose, using the OneTouch UltraMini blood glucose monitoring system from LifeScan Canada Ltd. (Vancouver, BC, Canada). Insulin was measured using a commercially available kit listed in the reagents section. All procedures were performed according to instructions provided by the manufacturer of the kit.
5
Measuring Blood Glucose, BNP, and Aldosterone
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Blood glucose levels were measured in fresh blood samples collected from the middle tail vein using OneTouch UltraMini blood glucose monitoring system and test strips (LifeScan). BNP and aldosterone levels were measured in blood plasma by ELISA using manufacturers' instructions (RayBiotech, EIAM‐BNP‐1 and Cayman Chemical, Aldosterone EIA, respectively). Three technical replicates were performed for each biological sample.
6
Glucose Tolerance Test in Mice
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