The HCC1806 and MDA-MB-231 cells were plated in ultra-low attachment 96-well plates with EpiCult-B Basal Medium (Human) (Stem Cell Technologies, BC, Canada) and EpiCult-B Proliferation Supplement (Human) (Stem Cell Technologies, BC, Canada) with hydrocortisone (H811182, Macklin, China) and heparin (Stem Cell Technologies, BC, Canada). The mammosphere formation efficiency was calculated after 10–14 days.
Hydrocortisone
Manufactured by Maclin Biochemical Technology
Sourced in Canada, China
Hydrocortisone is a synthetic corticosteroid used as a laboratory reagent. It functions as a glucocorticoid, primarily involved in the regulation of carbohydrate, fat, and protein metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Insulin, by Maclin Biochemical Technology (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
Epicult b basal medium human, by STEMCELL (1 mentions)
Epicult b proliferation supplement human, by STEMCELL (1 mentions)
Heparin, by STEMCELL (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cal27, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Wisent (1 mentions)
Cal27, by American Type Culture Collection (1 mentions)
Scc25, by American Type Culture Collection (1 mentions)
4 protocols using hydrocortisone
1
Mammosphere Formation Assay
2
Cell Line Cultivation Protocol for Breast and Kidney Cell Lines
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MCF10A, MCF7, T47D, and HEK293T cell lines were purchased from the Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences. MCF10A, a kind of normal human breast epithelial cell, was grown in DMEM/F12 (Gibco, Life, China) medium supplemented with 10% fetal bovine serum (FBS) (Gibco, Australia), 10 µg/ml insulin (Macklin, China), 20 ng/ml EGF (Peprotech, China), and 0.5 µg/ml hydrocortisone (Macklin, China). MCF7 and HEK293T cells were maintained in DMEM (Gibco, Life, USA) supplemented with 10% FBS (Gibco, Australia). T47D cells were maintained in RPMI-1640 medium (Gibco, Life, USA) supplemented with 10% FBS (Gibco, Australia). All medium were supplemented with 100 U/ml penicillin–streptomycin (Gibco, Life, China), and all cells were maintained at 37°C in a humidified atmosphere containing 5% CO2 and confirmed to be mycoplasma free.
3
Cell Culture Protocols for Cancer Research
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MCF10A, MC7F, T47D, and HEK293T cell lines were purchased from the Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences. MCF10A cells were grown in Dulbecco's modified eagle medium/nutrient mixture F-12 (DMEM/F12; 1:1, Gibco, Life, USA) medium supplemented with 10% fetal bovine serum (FBS) (Gibco, Australia), 10 μg/mL insulin (Macklin, China), 20 ng/mL epidermal growth factor (EGF) (Peprotech, China), 0.5 μg/mL hydrocortisone Macklin, China), and 100 U/mL penicillin-streptomycin. MCF7 cells were maintained in Dulbecco's modified eagle medium (DMEM; Gibco, Life, USA) supplemented with 10% FBS (Gibco, Australia) and 100 U/mL penicillin-streptomycin. T47D cells were maintained in Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Gibco, Life, USA) supplemented with 10% FBS (Gibco, Australia) and 100 U/mL penicillin-streptomycin. All cells were maintained at 37°C in a humidified atmosphere containing 5% CO2 and confirmed to be mycoplasma free.
4
HNSCC Tissue and Cell Line Protocol
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With the approval of the Stomatology Hospital Research Ethics Committee, twenty HNSCC samples and paired adjacent non-cancerous normal tissues (ANNT) were acquired from the Stomatology Hospital of Sun Yat-sen University. Informed consent was signed by each patient. The human HNSCC cell lines SCC25 and CAL27 were purchased from the American Type Culture Collection (ATCC). DMEM/F12 (Gibco, New York, NY, USA) medium containing 10% fetal bovine serum (FBS, WISENT, Montreal, QC, Canada) and 400 ng/mL hydrocortisone (MACKLIN, Shanghai, China) was used to culture SCC25 cells. In DMEM (Gibco, USA) medium containing 10% FBS, CAL27 cells were grown. Cells were cultured at 37 °C in a humidified 5% CO2 incubator.
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