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Quantikine mouse vegf immunoassay elisa kit

Manufactured by R&D Systems
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The Quantikine mouse VEGF immunoassay ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure mouse VEGF levels in cell culture supernates, serum, and plasma.

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4 protocols using quantikine mouse vegf immunoassay elisa kit

1

Quantification of VEGF165 in MSC Secretome

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VEGF165‐a in the cell culture supernatants was quantified using a Quantikine mouse VEGF immunoassay ELISA kit (R&D Systems, Minneapolis, MN, https://www.rndsystems.com). The cells were washed twice with PBS, and the media was changed to keratinocyte serum‐free medium (KSFM) for the hUCB‐MSC and VEGF/hUCB‐MSC (Dox−) groups. For the VEGF/hUCB‐MSCs (Dox+) group, 500 ng/ml of Dox was added. After incubation for 1 day, 1 ml of medium in a 60‐mm dish was harvested from each group. The conditioned media was filtered and analyzed by ELISA in duplicate. The results were normalized for the number of cells in the dish and the time of exposure to the cells.
Cho H., Kim P., Chang H., Shen Y., Bonsra K., Kang B., Yum S., Kim J., Lee S., Choi M., Kim H.(., Jang G, & Cho J. (2017). Targeted Genome Engineering to Control VEGF Expression in Human Umbilical Cord Blood‐Derived Mesenchymal Stem Cells: Potential Implications for the Treatment of Myocardial Infarction. Stem Cells Translational Medicine, 6(3), 1040-1051.
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2

Quantification of VEGF164 in Myoblasts

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The production of VEGF164 in cell culture supernatants was quantified by a Quantikine mouse VEGF Immunoassay ELISA kit (R&D Systems, Abingdon, UK). One milliliter of fresh medium was incubated for 4 h on myoblasts seeded overnight in a 60‐mm dish, filtered, and analyzed in duplicate. Results were normalized by the number of cells and time of incubation. Four dishes of cells were assayed per cell type (n = 4).
Groppa E., Brkic S., Uccelli A., Wirth G., Korpisalo‐Pirinen P., Filippova M., Dasen B., Sacchi V., Muraro M.G., Trani M., Reginato S., Gianni‐Barrera R., Ylä‐Herttuala S, & Banfi A. (2018). EphrinB2/EphB4 signaling regulates non‐sprouting angiogenesis by VEGF. EMBO Reports, 19(5), e45054.
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3

VEGF Quantification in Myoblasts

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The production of VEGF164 in cell culture supernatants was quantified using a Quantikine mouse VEGF Immunoassay ELISA kit (R&D Systems Europe, Abingdon, UK). 1 ml of medium was harvested from myoblasts in one 60-mm dish, following a 4-h incubation, filtered, and analyzed in duplicate. Results were normalized by the number of cells in the dish and time of exposure to medium. Four dishes of cells were assayed per cell type (n = 4).
Groppa E., Brkic S., Bovo E., Reginato S., Sacchi V., Di Maggio N., Muraro M.G., Calabrese D., Heberer M., Gianni-Barrera R, & Banfi A. (2015). VEGF dose regulates vascular stabilization through Semaphorin3A and the Neuropilin-1+ monocyte/TGF-β1 paracrine axis. EMBO Molecular Medicine, 7(10), 1366-1384.
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4

Quantifying Rabbit VEGF in Cell Culture

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The production of rabbit-VEGF in cell culture supernatants was quantified using a Quantikine mouse VEGF immunoassay ELISA kit (R&D Systems Inc., Minneapolis, MN, United States). One ml of fresh medium was incubated on rBMSCs cultured in 60-mm dishes in duplicate for 4 h, filtered, and frozen. Results were normalized by the number of cells in each dish and the time of incubation.
Largo R.D., Burger M.G., Harschnitz O., Waschkies C.F., Grosso A., Scotti C., Kaempfen A., Gueven S., Jundt G., Scherberich A., Schaefer D.J., Banfi A, & Di Maggio N. (2020). VEGF Over-Expression by Engineered BMSC Accelerates Functional Perfusion, Improving Tissue Density and In-Growth in Clinical-Size Osteogenic Grafts. Frontiers in Bioengineering and Biotechnology, 8, 755.
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