Whole blood was collected from the saphenous vein at regular time points. Blood was then allowed to clot for 30 min and the serum was separated by centrifugation at 1,500 × g for 10 min. The levels of cytokines in the serum were measured using the Quantibody Rat Cytokine Array 2 multiplex ELISA kit that quantitatively measured 10 rat inflammatory factors: ICAM-1, interferon γ, IL-1β, IL-6, IL-10, leptin, L-selectin, MCP-1, TIMP-1, and TNF-alpha (RayBiotech). All ELISA procedures were performed according to the manufacturer’s protocols.
Icam 1
Manufactured by RayBiotech
Sourced in United States
ICAM-1 is a cell surface glycoprotein that functions as an intercellular adhesion molecule. It plays a role in the adhesion of cells to the extracellular matrix or to other cells.
Automatically generated - may contain errors
Lab products found in correlation
Leptin, by RayBiotech (1 mentions)
Tnf alpha, by RayBiotech (1 mentions)
Timp 1, by RayBiotech (1 mentions)
Mcp 1, by RayBiotech (1 mentions)
Il 10, by RayBiotech (1 mentions)
Interleukin 6 (il 6), by RayBiotech (1 mentions)
Il 1β, by RayBiotech (1 mentions)
Type 7170a, by Hitachi (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
2 protocols using icam 1
1
Multiplex Cytokine Profiling in Rat Serum
2
Lipid Profile and Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood samples were collected from the jugular vein. Blood lipid analysis was performed before and after 8 weeks on the high-cholesterol diet and after 8 weeks of atorvastatin treatment. Plasma totalcholesterol (TC), LDL and total triglyceride (TG) concentrations were measured by an enzymatic method (BioMerieux, Lyon, France) using an automated analyzer (Type 7170A, Hitachi, Japan). Enzyme-linked immunosorbent assay (ELISA) kits were used to measure plasma levels of GGT-1 (Cloud-Clone Crop, USA), intercellular cell adhesion molecule-1 (ICAM-1, RayBiotech, USA), vascular cell-adhesion molecule-1 (VCAM-1, Fitzgerald, USA), interleukin-6 (IL-6, R&D Systems Inc., USA) and high-sensitivity C-reactive protein (hs-CRP, BioVendor R&D Systems Inc., CZ).
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