Fluorescein isothiocyanate fitc conjugated anti p selectin

Manufactured by Cell Signaling Technology

Fluorescein isothiocyanate (FITC)‐conjugated anti‐P‐selectin is a lab equipment product that consists of an antibody specific to P‐selectin, a cell adhesion molecule, conjugated to the fluorescent dye FITC. The primary function of this product is to allow for the detection and visualization of P‐selectin expression in various experimental and analytical applications.

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Lab products found in correlation

Fitc conjugated annexin 5, by Cell Signaling Technology (2 mentions) Jon a antibody, by Emfret Analytics (2 mentions) Phycoerythrin conjugated anti cd69, by BD (2 mentions) Fitc conjugated anti cd45, by BD (2 mentions) Adenosine diphosphate (adp), by Merck Group (2 mentions) Stir bars, by Chrono-Log (2 mentions) Thrombin, by Chrono-Log (2 mentions) U46619, by Abcam (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Nicotine, by Merck Group (1 mentions) Cotinine, by Merck Group (1 mentions) Adenosine diphosphate adp, by Merck Group (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

FITC Anti–P-selectin

3 protocols using fluorescein isothiocyanate fitc conjugated anti p selectin

Platelet Activation Assay with Vaping E-liquid

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Absolute Zero e‐liquid (18 mg nicotine, 30% propylene glycol, 70% vegetable glycerin with a menthol flavor) was obtained from The Vapor Chef (Bristol, PA). ADP, prostacyclin/prostaglandin I₂ (PGI₂), cotinine methanol solution, and cotinine‐D3 standard were purchased from Sigma Aldrich (St. Louis, MO). U46619 was purchased from Abcam (St. Louis, MO). Fluorescein isothiocyanate (FITC)‐conjugated anti‐P‐selectin and FITC‐conjugated Annexin V were purchased from Cell Signaling Technology, Inc (Danvers, MA). The JON/A antibody was obtained from Emfret analytics (Würzburg, Germany). The phycoerythrin‐conjugated anti‐CD69 and FITC‐conjugated anti‐CD45 were obtained from BD Biosciences (San Jose, CA). Stir bars and other disposables were purchased from Chrono‐Log Corporation (Havertown, PA). Kinetex 1.7 μm EVO C18 100 A‐LC Column 100×2.1 mm, Security Guard ULTRA holder for UHPLC Columns 2.1 to 6.4 mm, and SecurityGuard ULTRA cartridges for EVO‐C18‐UHPLC were purchased from Phenomenex Inc (Torrance, CA). Other reagents were of analytical grade.

Qasim H., Karim Z.A., Silva‐Espinoza J.C., Khasawneh F.T., Rivera J.O., Ellis C.C., Bauer S.L., Almeida I.C, & Alshbool F.Z. (2018). Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(15), e009264.

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Platelet Activation and Signaling Pathways

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Thrombin was purchased from Chronolog Corporation (Havertown, PA), whereas ADP, nicotine and cotinine were from Sigma Aldrich (St. Louis, MO). Fluorescein isothiocyanate (FITC)-conjugated anti-P-selectin and FITC-conjugated Annexin V were purchased from Cell Signaling Technology, Inc (Danvers, MA). The JON/A antibody was obtained from Emfret analytics (Würzburg, Germany). The phycoerythrin-conjugated anti-CD69 and FITC-conjugated anti-CD45 were obtained from BD Biosciences (San Jose, CA). Stir bars and other disposables were purchased from Chrono-Log Corporation (Havertown, PA). Akt, phospho-Akt (Ser473), ERK and phospho-ERK antibodies were purchased from Cell signaling (Danvers, MA). The anti-PAR4 and anti-GPIIb-IIIa (αIIbβ3) antibodies were from Abcam (Cambridge, MA), whereas the anti-p85/PI3K antibody was from Proteintech (Rosemont, IL). The ELISA cotinine detection kit was purchased from Calbiotech (El Cajon, CA). The fibrinogen plasma detection/quantification ELISA kit was purchased form Innovative Research (Novi, MI).

Alarabi A.B., Karim Z.A., Montes Ramirez J.E., Hernandez K.R., Lozano P.A., Rivera J.O., Alshbool F.Z, & Khasawneh F.T. (2020). Short-Term Exposure to Waterpipe/Hookah Smoke Triggers a Hyperactive Platelet Activation State and Increases the Risk of Thrombogenesis. Arteriosclerosis, thrombosis, and vascular biology, 40(2), 335-349.

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Platelet Activation and Cotinine Measurement

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Thrombin, chrono-lume mixture, stir bars, and other disposables were purchased from Chronolog Corporation (Havertown, PA), whereas adenosine diphosphate (ADP) was from Sigma Aldrich (St Louis, MO). Fluorescein isothiocyanate/FITC-conjugated anti-P-selectin, Akt (protein kinase B), and phospho-Akt (Ser473) antibodies were purchased from Cell Signaling Technology, Inc (Danvers, MA). The phycoerythrin-conjugated GPIIb-IIIa (αIIbβ3) antibody was obtained from Emfret Analytics (Würzburg, Germany). The enzyme-linked immunosorbent assay (ELISA) cotinine detection kit was purchased from Calbiotech (El Cajon, CA). The e-liquid, e-head, and other e-waterpipe accessories were purchased from Smoking hookah (Houston, TX).

Badejo P.O., Umphres S.S., Ali H.E.A., Alarabi A.B., Qadri S., Alshbool F.Z, & Khasawneh F.T. (2024). Exposure to Electronic Waterpipes Increases the Risk of Occlusive Cardiovascular Disease in C57BL/6J Mice. Journal of cardiovascular pharmacology and therapeutics, 29.

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