Vs120 s6 w slide scanning microscope

Manufactured by Olympus

The VS120-S6-W slide scanning microscope is a high-performance digital slide scanning system designed for use in research and clinical laboratories. The core function of this microscope is to capture high-resolution images of entire microscope slides, enabling efficient and comprehensive digital analysis of samples.

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Lab products found in correlation

Epr21143, by Abcam (1 mentions) Cd3 12, by Abcam (1 mentions) Anti rabbit igg af594, by Abcam (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Vector blue, by Vector Laboratories (1 mentions) Abc ap reagent, by Vector Laboratories (1 mentions)

Close spelling variants of this product

VS120 slide scanning microscope VS120-S6-W VS120-S6 Slide scanning microscope VS120 microscope VS120

2 protocols using vs120 s6 w slide scanning microscope

Multiplex Immunofluorescence Profiling of Melanoma

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Sections of frozen metastatic melanoma tumor tissue were thawed at room temperature (RT) and fixed with pre-cooled Acetone and Ethanol for 10 minutes each. Sections were washed and incubated with blocking buffer (0.1% BSA in 0.1% TBS-T) for 1 hour at RT, prior to primary antibody incubation for 1 hour at RT with one of either 5µg/ml rabbit anti-IL-10 (polyclonal, Abcam), 8µg/ml rabbit anti-TNF-α (TNFA/1500R, Abcam), or 1µg/ml rabbit anti-TGFβ1 (EPR21143, Abcam) in addition to 10µg/ml rat anti-CD3 (CD3-12, Abcam) and mouse anti-CD20 (1/50 dilution, L26, Abcam). Sections were washed and incubated with 10µg/ml cross-adsorbed donkey secondary antibodies (Abcam): anti-rabbit IgG AF594, anti-mouse IgG AF488 and anti-rat IgG AF647. Sections were washed and mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific). Slides were incubated for 24 hours prior to visualization on the Olympus VS120-S6-W slide scanning microscope.

Harris R.J., Willsmore Z., Laddach R., Crescioli S., Chauhan J., Cheung A., Black A., Geh J.L., MacKenzie Ross A.D., Healy C., Tsoka S., Spicer J., Lacy K.E, & Karagiannis S.N. (2022). Enriched circulating and tumor-resident TGF-β+ regulatory B cells in patients with melanoma promote FOXP3+ Tregs. Oncoimmunology, 11(1), 2104426.

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Immunohistochemical Analysis of TMEM97 in Human Eyes

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Eyes from individuals without a history or evidence of retinal disease upon gross examination were obtained within 12 h of death and preserved in formalin and embedded in paraffin. Five-micron thick sections were prepared for immunohistochemistry by bleaching pigment using oxalic acid/potassium permanganate solution followed by heat-induced epitope retrieval with Tris-EDTA buffer (pH 9) at 60 °C for 1 h. Immunohistochemical staining of bleached eye sections was performed with rabbit anti-human TMEM97 antibody (Proteintech Cat. No. 26444–1-AP, diluted 1:100 in DAKO antibody diluent) overnight at 4 °C. Rabbit IgG isotype control was applied to adjacent sections in place of the primary antibody to ascertain the specificity of staining. Slides were incubated with a biotin-conjugated secondary antibody, followed by incubation with VECTASTAIN® ABC-AP reagent and development using Vector Blue (Vector Laboratories, Cat No. AK-5000 and SK-5300, respectively). Images were obtained using an Olympus VS120-S6-W slide-scanning microscope.

Shen H., Li J., Heisler-Taylor T., Makin R., Yang H., Mavlyutov T.A., Gelfand B., Cebulla C.M, & Guo L.W. (2021). TMEM97 ablation aggravates oxidant-induced retinal degeneration. Cellular signalling, 86, 110078.

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