Iron(III) acetylacetonate (Fe(acac)3), 1,2-hexadecanediol, oleylamine, polyethylene glycol (PEG), oleic acid, benzyl ether, cetyl trimethyl ammonium bromide (CTAB), HAuCl4, NaBH4, l -ascorbic acid, agar and doxorubicin (DOX) were purchased from J&K Scientific Ltd. Glycolide and Lactide were purchased from Purac (Netherlands) and further purified by recrystallization from ethyl acetate for several times. Stannous octoate, polyvinyl alcohol (PVA) and fluorescein diacetate were purchased from Sigma Aldrich. Fast membrane emulsification equipment (FMEM-500 M) and Shirasu porous glass (SPG) membrane were purchased from National Engineering Research Center for Biotechnology (Beijing). The glass membranes were annular cylinders (inner diameter = 8 mm, external diameter = 10 mm, length = 170 mm) with pore sizes of 7.2 μm. All other reagents were purchased from Beijing Chemical Reagents Company and used as received without further purification. Cells were supplied by China Infrastructure of Cell Line Resources.
L ascorbic acid
Manufactured by J&K Scientific
Sourced in Netherlands
L-ascorbic acid is a chemical compound that is commonly known as vitamin C. It is a white crystalline solid that is soluble in water. L-ascorbic acid is an essential nutrient that plays a crucial role in various biological processes, including immune function, collagen production, and antioxidant protection.
Automatically generated - may contain errors
Lab products found in correlation
Cetyl trimethyl ammonium bromide, by J&K Scientific (1 mentions)
Fluorescein diacetate, by Merck Group (1 mentions)
Oleylamine, by J&K Scientific (1 mentions)
Oleic acid, by J&K Scientific (1 mentions)
Haucl4, by J&K Scientific (1 mentions)
Nabh4, by J&K Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Stannous octoate, by Merck Group (1 mentions)
Glycerophosphate, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
2 protocols using l ascorbic acid
1
Synthesis of Multicomponent Nanoparticles
2
Osteogenic Differentiation of HPDLSCs
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HPDLSCs were induced in an α‐MEM medium containing 5% foetal bovine serum, 1% penicillin–streptomycin, 0.02 μM dexamethasone (Sigma–Aldrich), 50 μg/ml l ‐ascorbic acid (J&K), and 8 mM glycerophosphate (Sigma–Aldrich). After 3 and 7 days of culture, an alkaline phosphatase (ALP) assay was used to detect osteogenic differentiation. After 14 days of culture, alizarin red staining was used to evaluate calcified nodule formation in the extracellular matrix. RNA and protein expressions of osteogenesis‐related markers were detected by qRT‐PCR and Western blot on day 3 and day 7, respectively.
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