Anti cd11c bv650

Manufactured by BD

Sourced in United States

Anti-CD11c-BV650 is a fluorescence-labeled antibody that binds to the CD11c surface marker, which is expressed on dendritic cells and some other leukocyte populations. This product is intended for use in flow cytometry applications to identify and analyze CD11c-positive cells.

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Lab products found in correlation

Lenalidomide, by Abcam (1 mentions) Anti pd 1 eh12.2h7, by BioLegend (1 mentions) Anti cd4 bv605, by BD (1 mentions) Anti cd4 bv786, by BD (1 mentions) Is5 21f5, by Miltenyi Biotec (1 mentions) Anti cd19 pe cf594, by BD (1 mentions) Anti cd11b percp cy5, by BD (1 mentions) Anti cd40 pe, by BD (1 mentions) Anti mhcii fitc, by BD (1 mentions) Cellquest software, by BD (1 mentions) Anti cd86 pe cy7, by BD (1 mentions) Anti cd80 apc, by BD (1 mentions) Facscalibur, by BD (1 mentions)

2 protocols using anti cd11c bv650

Multicolor Flow Cytometry for Immune Checkpoint Analysis

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The following fluorochrome-conjugated mAbs were used for multicolor flow cytometry: anti-CD3-Brilliant Violet™ (BV) 786 (SK7), anti-CD4-BV786 (RPA-T4), anti-CD4-BV605 (RPA-T4), anti-CD8-allophycocyanin (APC)-H7 (RPA-T8), anti-CD14-BV605 (M5E2), anti-CD11c-BV650 (B-ly6), anti-CD19-PE-CF594 (HIB19), anti-HLA-antigen-D related (DR)-PerCP-Cy5.5 (G46-6), anti-PD-L1-PE-Cy7 (MIH1), anti-PD-L2-PE (MIH18) (BD Bioscience, San Jose, CA, USA), anti-PD-1-BV421 (EH12.2H7), anti-CD3-APC (SK7), anti-T-cell immunoglobulin and mucin-domain containing-3 (Tim-3)-PE (F38-E2E) (Biolegend, San Jose, CA, USA) and anti-lymphocyte activation gene-3 (Lag-3)-PE-eFluor610 (3DS223H) (Invitrogen, Carlsbad, CA, USA). For functional assays, anti-PD-1 (EH12.2H7, Biolegend) and mouse IgG1 isotype control Abs (IS5-21F5, Miltenyi Biotec, Bergisch Gladbach, Germany) were used. TGF-β receptor-1 inhibitor (galunisertib, LY2157299) was purchased from Selleckchem Chemicals (Houston, TX, USA) and lenalidomide from Abcam (Cambridge, MA, USA).

Kwon M., Choi Y.J., Sa M., Park S.H, & Shin E.C. (2018). Two-Round Mixed Lymphocyte Reaction for Evaluation of the Functional Activities of Anti-PD-1 and Immunomodulators. Immune Network, 18(6), e45.

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Dendritic Cell Maturation Assay

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Immature BMDC were cocultured with mitomycin C-treated MC38/0, MC38/shN, and MC38/shTGFβ1-1, 2, 3 cells in the presence of GM-CSF (40 ng/ml). After 24 h, dendritic cells were harvested and labeled with monoclonal antibodies conjugated with fluorochromes (BD Biosciences): anti-CD40 PE, anti-CD80 APC, anti-CD86 PE-Cy7, anti-MHC II FITC, anti-CD11b PerCP-Cy5.5, and anti-CD11c BV650. The expression of cell surface markers was analyzed using FACS Calibur with CellQuest software (Becton Dickinson).

Rossowska J., Anger N., Szczygieł A., Mierzejewska J, & Pajtasz-Piasecka E. (2017). Intratumoral Lentivector-Mediated TGF-β1 Gene Downregulation As a Potent Strategy for Enhancing the Antitumor Effect of Therapy Composed of Cyclophosphamide and Dendritic Cells. Frontiers in Immunology, 8, 713.

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