Six- and 12-well TCPS plates were coated with a range of defined substrates according to the manufacturer’s recommendations. Vitronectin (provided by the lab of Dr. Jamie Thomson, University of Wisconsin-Madison) or laminin-521 (BioLamina) were diluted in PBS and coated onto TCPS plates at a density of 0.61 μg/cm2 overnight at 37°C. Synthemax (Corning) was coated onto TCPS at a density of 3.3 μg/cm2 in PBS and incubated overnight at 37°C. StemAdhere coatings were achieved by diluting 240 μl StemAdhere (STEMCELL Technologies) in 6 ml of StemAdhere dilution buffer, with 1 ml of this solution (1.02 μg/cm2) placed into each well of a 6-well non-tissue culture polystyrene plate (BD Falcon) and incubated overnight at 37°C. Prior to cell seeding, all plates were washed with sterile PBS to remove any non-adsorbed coating.
Vitronectin
Manufactured by BioLamina
Vitronectin is a glycoprotein that serves as a cell attachment factor. It is involved in the regulation of the complement system and plays a role in cell adhesion, migration, and survival. Vitronectin supports the attachment and growth of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Laminin 521, by BioLamina (2 mentions)
Synthemax, by Corning (1 mentions)
Stemadhere, by STEMCELL (1 mentions)
Collagen type 1, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Human fibronectin, by Merck Group (1 mentions)
Laminin, by Merck Group (1 mentions)
Vitronectin, by Merck Group (1 mentions)
2 protocols using vitronectin
1
Defined Substrate Coatings for Cell Culture
2
Extracellular Matrix Protein Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Collagen type I (100 mg) (from rat tail), lyophilized fibronectin (5 mg) (from rat plasma), vitronectin (50 μg) (from human plasma) and laminin (0.6 mg) (from human fibroblast) (all from Sigma) were dissolved in 1 ml deionized water according to the manufacturer’s instruction.
The ECM proteins used in BLI measurements are rat collagen I, 4 mg/ml in 50 mM acetic acid (Thermo Fisher Scientific), human fibronectin, 1 mg/ml, in PBS (Sigma), laminin 521, 0.1 mg/ml in PBS (BioLamina), vitronectin, 0.22 mg/ml in TBS (a gift from late Dr. Rupert Timpl, Max Planck Institute of Biochemistry), and a reference protein, heat-shocked BSA, 10 mg/ml in dH2O (Sigma).
The ECM proteins used in BLI measurements are rat collagen I, 4 mg/ml in 50 mM acetic acid (Thermo Fisher Scientific), human fibronectin, 1 mg/ml, in PBS (Sigma), laminin 521, 0.1 mg/ml in PBS (BioLamina), vitronectin, 0.22 mg/ml in TBS (a gift from late Dr. Rupert Timpl, Max Planck Institute of Biochemistry), and a reference protein, heat-shocked BSA, 10 mg/ml in dH2O (Sigma).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!