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Aspartate aminotransferase ast

Manufactured by Merck Group
Sourced in United States, Germany

Aspartate aminotransferase (AST) is a laboratory test used to measure the levels of the enzyme AST in the blood. AST is an enzyme found primarily in the liver and heart, and its measurement can provide information about the health of these organs.

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2 protocols using aspartate aminotransferase ast

1

Immune and Biochemical Markers Evaluation

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Blood samples were collected into non-heparinized and K3-EDTA vacuum
tubes (Becton Dickinson Vacutainer Systems) to determine numbers of immune
cells, including leukocytes, lymphocytes, and monocytes, after 3 and 4 weeks.
Immune cell counts were determined using an automatic blood analyzer (ADVIA120;
Bayer, Leverkusen, Germany). ELISA was used to quantify serum concentrations of
tumor necrosis factor (TNF)-α (R&D Systems,
Minneapolis, MN, USA), alanine transaminase (ALT; Sigma-Aldrich, St. Louis, MO,
USA), aspartate aminotransferase (AST; Sigma-Aldrich, St. Louis, MO, USA),
superoxide dismutase (SOD; Cohesion Biosciences, USA), malondialdehyde (MDA;
Abcam, Cambridge, UK), and glutathione peroxidase (GPx; Cayman Chemical, Ann
Arbor, MI, USA).
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2

Lipid Profile and Liver Enzyme Assay

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Formalin, hematoxylin, eosin, ethanol, paraffin, chloroform and diethyl ether were bought from Merck Co., (Germany). Kits for the measurement of triacylglycerol (TAG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were obtained from Sigma-Aldrich (Steinheim, Germany). Standard choline-deficient diet (CDD) was supplied from Harlan Teklad (Milano, Italy).
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