Orca flash 4.0 lt digital cmos camera

Fluorescent polystyrene particles (0.70, 0.79, 0.92 and 1.0 µm, Thermo Scientific Fluoro-Max) were suspended in deionized water (with 0.1% of Triton X to reduce agglomeration) in a concentration of ∼ 0.001 vol%.
An HPLC pump (Waters, model 515) was used to pump the samples through the devices at a controlled flow rate with a read out of the pressure.
During the operation, the devices were observed with an inverted fluorescence microscope (Olympus IX73 with an Orca-Flash 4.0 LT digital CMOS camera). Images were taken with a magnification of 20X and a 2 s exposure time. The intensity profiles were analysed by ImageJ.

Muscle cross-sections (10 µm thick) were obtained using a cryostat (HM500M Microm International, Fisher Scientific, Illkirch, France) at −20 °C. Cross-sections were labeled with anti-laminin-α1 (L9393 Sigma, Saint-Quentin-Fallavier, France) to outline the fibers, and resolved with corresponding secondary antibodies conjugated to Alexa-Fluor 488 (Invitrogen, Cergy-Pontoise, France). Observations and image acquisitions were captured with a high-resolution ORCA-Flash4.0 LT+ Digital CMOS camera coupled to an IX-73 microscope (Olympus) and Cell-Sens dimension software (Olympus Soft Imaging Solutions, Münster, Germany). The cross-sectional area (CSA) was determined for each fiber, using ImageJ 1.53f51 (http://rsb.info.nih.gov/ij/ accessed on 17 May 2022).