The human GIST cell lines, GIST-882 and GIST-T1, were purchased from the Cosmo Bio, Co., Ltd., (Tokyo, Japan) and were not passaged for longer than 6 months continuously. GIST cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). All culture media were supplemented with 10% heat-inactivated fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 mg/mL streptomycin. All cells were maintained at 37 °C in a humidified incubator with 5% CO2
Gist 882
Manufactured by Cosmo Bio
Sourced in Japan
GIST-882 is a laboratory instrument used for DNA/RNA analysis. It is designed to perform gel electrophoresis for the separation and visualization of nucleic acid fragments. The core function of GIST-882 is to facilitate the separation and analysis of DNA or RNA samples.
Automatically generated - may contain errors
2 protocols using gist 882
1
GIST Cell Line Culturing Protocol
2
Silencing AOC4P in GIST Cells
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GIST-T1 (heterozygosis mutation type of KIT exon) and GIST-882 (homozygotic mutation type of KIT exon) cells were purchased from Cosmo Bio (Tokyo, Japan) and cultured in DMEM medium (Hyclone, USA) containing 10% fetal bovine serum and 1% penicillin streptomycin (Gibco, USA) at 37°C incubator (Forma, USA) containing 5% of CO2.
GIST-T1 and GIST-882 cells were inoculated into a six-well culture plate in a 37°C incubator overnight. The experiment had three groups to test the effect of AOC4P on GIST including negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). Lipidosome2000 (Amersham Bioscience, Piscataway, NJ, USA) was used to transfect 1 µg si AOC4P and 1 µg si RNA in cells as si AOC4P and si CT group, respectively. The sequences of si-AOC4P are as follows: sense: 5′-GGUAAGGCAUCCUGGCAGAUU-3′ and antisense: 5′-CCAGAGGUAUGGAAACAAAUU-3′.
GIST-T1 and GIST-882 cells were inoculated into a six-well culture plate in a 37°C incubator overnight. The experiment had three groups to test the effect of AOC4P on GIST including negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). Lipidosome2000 (Amersham Bioscience, Piscataway, NJ, USA) was used to transfect 1 µg si AOC4P and 1 µg si RNA in cells as si AOC4P and si CT group, respectively. The sequences of si-AOC4P are as follows: sense: 5′-GGUAAGGCAUCCUGGCAGAUU-3′ and antisense: 5′-CCAGAGGUAUGGAAACAAAUU-3′.
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