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Kgm media

Manufactured by Lonza
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KGM media is a specialized culture medium designed for the growth and maintenance of keratinocytes, which are the predominant cell type in the epidermis of the skin. The media provides the necessary nutrients and growth factors to support the proliferation and differentiation of keratinocytes in vitro.

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Lab products found in correlation

Staurosporine, by Abcam (1 mentions) Ex 527, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Dmem f12, by Sartorius (1 mentions) M154 media, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions) Rosiglitazone, by Bio-Techne (1 mentions) Rosiglitazone, by Merck Group (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) T0070907, by Bio-Techne (1 mentions) Ksfm media, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Rosiglitazone, by Lonza (1 mentions)

Spelling variants of this product

KGM-Gold media (6 citations) KGM-2 media (4 citations)

5 protocols using kgm media

1

Calcium-induced Keratinocyte Differentiation

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Cells were maintained in E-media until reaching 60–80% confluence, then washed with PBS and changed to KGM media (Lonza), to wash out calcium. After 24 hours, cells were changed to KBM (Lonza) supplemented with 1.5mM CaCl2. After 48 hours in high-calcium, media was changed and at 48, 72 or 96 hours, cells were harvested.
Langsfeld E.S., Bodily J.M, & Laimins L.A. (2015). The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes. PLoS Pathogens, 11(9), e1005181.
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2

Differentiation and Apoptosis of CIN612 Cells

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CIN612 cells were plated at approximately 5x105 cells per 100mm dish and allowed to grow overnight in E media. The next day, 80μM EX-527 (Sigma) or equal volume of DMSO (Sigma) vehicle control were added to new E media and added to cells. The next day, cells to be differentiated were changed to KGM media (Lonza) to wash out calcium and after overnight incubation, were maintained in KBM media (Lonza) supplemented with 1.5mM CaCl2 and 80μM EX-527 or DMSO. Calcium and drug-containing media was changed every 48 hours until harvest. Undifferentiated cells were harvested after 48 hours of drug treatment.
Staurosporine was purchased from Abcam and CIN612 cells treated with 500nM or equal volume DMSO control for 4 hours to induce apoptosis.
Langsfeld E.S., Bodily J.M, & Laimins L.A. (2015). The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes. PLoS Pathogens, 11(9), e1005181.
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3

Extraction and Cultivation of Human Keratinocytes

Cited 1 time
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NHEKs were extracted from skin discarded during plastic surgery procedures, after written informed consent had been obtained as previously described [43 (link)]. The keratinocytes were seeded on feeder plates containing 3T3-J2 fibroblasts and were grown in medium Green containing 42.5% DMEM (Biological Industries, Beit-Haemek, Israel), 42.5% DMEM/F12 (Biological Industries, Beit-Haemek, Israel), 10% FCII serum, 1% penicillin-streptomycin, 1mM L-glutamine, 1mM sodium pyruvate, 5 μg/mL Insulin, 0.2 mM adenine, 0.5 μg/mL hydrocortisone, 2nM Triiodothyronine, 0.1 nM cholera toxin and 10 ng/mL EGF, and were frozen upon 90% confluence. Cell were then thawed and cultured in KGM media (Lonza, Basel, Switzerland).
For the dispase-based dissociation assay, NHEKs were extracted from foreskin using the same conditions and were thawed and cultured in M154 media (Life Technologies, Carlsbad, CA).
Vodo D., Sarig O., Geller S., Ben-Asher E., Olender T., Bochner R., Goldberg I., Nosgorodsky J., Alkelai A., Tatarskyy P., Peled A., Baum S., Barzilai A., Ibrahim S.M., Zillikens D., Lancet D, & Sprecher E. (2016). Identification of a Functional Risk Variant for Pemphigus Vulgaris in the ST18 Gene. PLoS Genetics, 12(5), e1006008.
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4

Keratinocyte Differentiation Induction

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Primary and immortalized HFKs were cultured onto 100 mm dish to full confluency in KGM media (Lonza). After confirmation of cell density, cells were switched to differentiation induction media (1.8 mM CaCl2 and 10% FBS). After 4 days and 7 days, pictures of differentiated cells were taken and their lysates were prepared further analysis.
Choi M., Park M., Lee S., Lee J.W., Cho M.C., Noh M, & Lee C. (2017). Establishment of Immortalized Primary Human Foreskin Keratinocytes and Their Application to Toxicity Assessment and Three Dimensional Skin Culture Construction. Biomolecules & Therapeutics, 25(3), 296-307.
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5

Meibocyte Differentiation Protocol

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Immortalized hMGEC, a generous gift from Dr. Sullivan (Schepens Eye Research Institute), was grown in KSFM media (Invitrogen-Gibco, Grand Island, NY) as previously described [10 (link)]. At 80% confluency, differentiation was induced by DMEM-F12 (Gibco, Grand Island, NY) supplemented with Epidermal Growth Factor (EGF, 10 ng/ml, Sigma) and various concentrations of PPARγ agonist, rosiglitazone (10–50 μM, Sigma, St. Louis, MO). After determining the maximum dose that induced lipogenesis and meibocyte differentiation, 30 μM rosiglitazone was used to induce meibocyte differentiation to measure gene expression and effects of PPARγ antagonists. Culture media was changed every other day and differentiation conditions were maintained for 1–6 days depending on analysis. For PPARγ signaling studies, a specific PPARγ antagonist, 10 μM of T0070907 (Tocris, Minneapolis, MN) was added 1 h prior to rosiglitazone treatment. As a comparison, gene expression and lipid synthesis following rosiglitazone exposure were also investigated in a human telomerized corneal epithelial cell line (hTCEpi), which were maintained in KGM media (Lonza Walkersville, Inc., Walkersville, MD) as previously described [16 (link)].
Kim S.W., Xie Y., Nguyen P.Q., Bui V.T., Huynh K., Kang J.S., Brown D.J, & Jester J.V. (2018). PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC). The ocular surface, 16(4), 463-469.
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