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Ellman s reagent 5 5 dithio bis 2 nitrobenzoic acid reduced glutathione 1 1.3 3 tetramethoxypropane

Manufactured by Merck Group
Sourced in Sao Tome and Principe, United States

Ellman's reagent [5,5-Dithio-bis-(2-nitrobenzoic acid)], reduced glutathione, and 1,1,3,3-tetramethoxypropane are laboratory reagents used in various biochemical and analytical applications. Ellman's reagent is a colorimetric agent used to quantify thiol groups, reduced glutathione is a common antioxidant, and 1,1,3,3-tetramethoxypropane is a standard compound used in assays. These products are intended for research use only and their specific applications should be determined by the user.

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2 protocols using ellman s reagent 5 5 dithio bis 2 nitrobenzoic acid reduced glutathione 1 1.3 3 tetramethoxypropane

1

Oxidative Stress Measurement Protocol

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Dimethyl sulfoxide (DMSO), RPMI-1640 Medium, fetal bovine serum (FBS), Penicillin/Streptomycin antibiotic, trypsin-EDTA, Ellman’s reagent [5,5-Dithio-bis-(2-nitrobenzoic acid)], reduced glutathione,1,1.3,3-tetramethoxypropane, trichloroacetic acid (TCA), thiobarbituric acid, β-mercaptoethanol, sodium dodecyl sulfate (SDS), sodium bicarbonate, methanol, and sulforhodamine B were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). Acetonitrile was obtained from Alliance Bio Co., USA. All other chemicals and solvents used were of the highest purity grade available.
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2

Oxidative Stress Biomarker Evaluation

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Dimethyl sulfoxide DMSO), RPMI-1640 medium, fetal bovine serum (FBS), Penicillin/ Streptomycin antibiotic, trypsin-EDTA, Ellman's reagent [5,5-Dithio-bis-(2-nitrobenzoic acid)], reduced glutathione,1,1.3,3-tetramethoxypropane, trichloroacetic acid (TCA), thiobarbituric acid, β-mercaptoethanol, sodium dodecyl sulfate (SDS),sodium bicarbonate and methanol were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acetonitrile was obtained from (Alliance Bio Co., USA). All other chemicals and solvents used were of the highest purity grade available.
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