Immunoblotting was performed with the following Santa Cruz Biotechnology (Dallas, TX, USA) antibodies: MLK1 (N-20) sc-19120, MLK3 (C-20) sc-536, B-Raf (F-3) sc-55522, MEK1/2 (12-B) sc-436, ERK2 (C-14) sc-154, JNK1 (C-17) sc-474, GST (B-14) sc-138, β-actin (C-4) sc-47778, and goat anti-rat IgG-HRP sc-2032. Activation state antibodies from Cell Signaling Technology (Beverly, MA, USA) include: phosphorylated MEK1/2 (p-MEK) (Ser221) 166F8, phosphorylated ERK1/2 (p-ERK) (Thr202/Tyr204) #4370S, and phosphorylated JNK (p-JNK) (Thr183/Tyr185) 9251L. Other antibodies used in this study were MLK4 NBP1-41081 (Novus Biologicals, Littleton, CO, USA), rat anti-DYKDDDDK (FLAG) 200474-21 (Agilent Technologies, Santa Clara, CA, USA), anti-rabbit IgG HRP conjugate W401B (Promega), and Immun-Star goat anti-mouse IgG-HRP conjugate 1705047 (Bio-Rad, Hercules, California, USA).
Anti rabbit igg hrp conjugate w401b
Manufactured by Promega
Sourced in United States
The Anti-rabbit IgG HRP conjugate W401B is a laboratory reagent used for the detection of rabbit immunoglobulin G (IgG) in various analytical techniques. It consists of an HRP (horseradish peroxidase) enzyme conjugated to anti-rabbit IgG antibodies, which enables the visualization and quantification of rabbit IgG in samples.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rat igg hrp sc 2032, by Santa Cruz Biotechnology (2 mentions)
Activation state antibodies, by Cell Signaling Technology (2 mentions)
Phosphorylated mek1 2 p mek ser221 166f8, by Cell Signaling Technology (2 mentions)
Mlk4 nbp1 41081, by Novus Biologicals (2 mentions)
Rat anti dykddddk flag 200474 21, by Agilent Technologies (2 mentions)
Immun star goat anti mouse igg hrp conjugate 1705047, by Bio-Rad (2 mentions)
β actin c 4 sc 47778, by Santa Cruz Biotechnology (2 mentions)
Ecl method, by PerkinElmer (1 mentions)
Anti sappβ mbs492139, by MyBioSource (1 mentions)
W02 antibody, by Merck Group (1 mentions)
Anti mouse p0260, by Agilent Technologies (1 mentions)
Anti actin ab1801, by Abcam (1 mentions)
Image gauge v3, by Fujifilm (1 mentions)
3 protocols using anti rabbit igg hrp conjugate w401b
1
Immunoblotting Analysis of Signaling Pathways
2
Immunoblotting Analysis of Signaling Pathways
3
Quantitative Analysis of BACE1, Aβ, and sAPPβ
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Samples used for the WB experiments were adjusted to equal protein concentration in advance.
For the determination of BACE1, cell lysates were prepared by lysing cells in 150 mM NaCl, 50 mM Tris/HCl pH 7.4, 2 mM EDTA, 0.1% NP-40, 0.1% Triton-X 100. For the determination of total secreted Aβ level and sAPPβ conditioned media were used.
The following antibodies were used for WB analysis: W02 antibody (5 μg/mL; Millipore, Billerica, MA, USA), anti-sAPPβ: Mbs492139 (1:250; MyBioSource, San Diego, CA, USA), BACE1: ab2077 (1:1000; abcam, Cambridge, UK), anti-actin ab1801 (1:1000; abcam), anti-rabbit IgG HRP Conjugate W401B (1:5000; Promega, Mannheim, Germany) and anti-mouse P0260 (Dako, Hamburg, Germany).
Aβ levels were detected by performing immunoprecipitation of conditioned media before WB analysis. Therefore, 20 μL protein G-Sepharose and W02 antibody (5 μg/mL) were used.
Enhanced chemiluminescense (ECL)-method (Perkin Elmer, Rodgau-Jügesheim, Germany) was used to detect proteins. Densitometrically quantification was performed by using Image Gauge V3.45 software (Fujifilm, Düsseldorf, Germany).
For the determination of BACE1, cell lysates were prepared by lysing cells in 150 mM NaCl, 50 mM Tris/HCl pH 7.4, 2 mM EDTA, 0.1% NP-40, 0.1% Triton-X 100. For the determination of total secreted Aβ level and sAPPβ conditioned media were used.
The following antibodies were used for WB analysis: W02 antibody (5 μg/mL; Millipore, Billerica, MA, USA), anti-sAPPβ: Mbs492139 (1:250; MyBioSource, San Diego, CA, USA), BACE1: ab2077 (1:1000; abcam, Cambridge, UK), anti-actin ab1801 (1:1000; abcam), anti-rabbit IgG HRP Conjugate W401B (1:5000; Promega, Mannheim, Germany) and anti-mouse P0260 (Dako, Hamburg, Germany).
Aβ levels were detected by performing immunoprecipitation of conditioned media before WB analysis. Therefore, 20 μL protein G-Sepharose and W02 antibody (5 μg/mL) were used.
Enhanced chemiluminescense (ECL)-method (Perkin Elmer, Rodgau-Jügesheim, Germany) was used to detect proteins. Densitometrically quantification was performed by using Image Gauge V3.45 software (Fujifilm, Düsseldorf, Germany).
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