Eight amino acids including tyrosine, tryptophan, threonine, glutamic acid, aspartic acid, histidine, phenylalanine, and arginine (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) were added into a 30% methanol aqueous solution of azalomycin F (100 μM) to obtain a mixed solution by vortexing, according to the molar concentration ratio of azalomycin F to each amino acid of 1:8. Simultaneously, the 30% methanol aqueous solution that only contained 100 μM azalomycin F was prepared as a blank control for background subtraction. After being incubated in a water bath at 37 °C for 30 min, these solutions were analyzed by a TripTOF 5600+ mass spectrometry (AB Sciex, Framingham, MA, USA) with an electrospray ion resource, and the detailed parameters are shown in Table S3 for negative ionization modes.
Histidine
Manufactured by Sinopharm
Sourced in China
Histidine is a naturally occurring amino acid that is commonly used in laboratory settings. It serves as a key component in the buffers and solutions used in various biochemical and analytical procedures. Histidine helps maintain appropriate pH levels and facilitate the stability of biomolecules in these applications.
Automatically generated - may contain errors
Lab products found in correlation
Threonine, by Sinopharm (2 mentions)
Glutamic acid, by Sinopharm (1 mentions)
Tyrosine, by Sinopharm (1 mentions)
Tryptophan, by Sinopharm (1 mentions)
Phenylalanine, by Sinopharm (1 mentions)
Aspartic acid, by Sinopharm (1 mentions)
N octane, by Sinopharm (1 mentions)
Potassium hydroxide, by Sinopharm (1 mentions)
Valine, by Sinopharm (1 mentions)
Dulbecco s modi ed eagle s medium dmem, by Merck Group (1 mentions)
Serine, by Sinopharm (1 mentions)
Ovalbumin (ova), by Sinopharm (1 mentions)
4 protocols using histidine
1
Azalomycin F Interaction with Amino Acids
2
Organometallic Catalysis for Desulfurization
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Tetraethyl orthosilicate (TEOS), lysine, histidine, absolute ethanol, n-octane, potassium hydroxide, niobium pentachloride, benzothiophene (BT), dibenzothiophene (DBT), 4, 6-dimethyldibenzothiophene (4, 6-DMDBT), N-methylpyrrolidone (NMP), hydrogen peroxide (30 wt%), etc., were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All chemicals were of analytical grade and used without any further purification.
3
Quantification of Metabolites by ESI-MS
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Both methanol and acetic acid were HLPC grade and bought from ROE Scientfic Inc. (Newark, U.S.A). All amino acids authentic compounds such as valine, histidine, arginine and lysine were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Creatine, L-carnitine (BR) and vitamin B6 (BR) were purchased from Shanghai Source Leaf Biological Technlogy Co., Ltd (Shanghai, China) with 98%, 98% and 99% purity, respectively. The deionized water used for experiments was provided by a Milli-Q water purification system (Billerica, USA). All standard solutions were prepared in deionized water and then used for ESI-MS analysis without further treatment.
4
Enzymatic Assays with Diverse Biomolecules
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All purchased reagents were of analytical reagent grade and utilized as received from the commercial suppliers without further puri cation. Doubly distilled water was utilized for the preparation of all aqueous solutions. Catechol, urea, citric acid, anhydrous ethanol, tyrosinase (TYR), and 3-(4,5-dimethyl-2thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Aladdin Reagent Co., Ltd (Shanghai, China). Histidine (His), Serine (Ser), Alanine (Ala), Threonine (Thr), Tyrosine (Tyr), Cysteine (Cys), Cystine (Cys-Cys), Soy protein isolate (SPI), Peanut protein isolate (PPI), Ovalbumin (OVA), Bovine serum albumin (BSA), Glucose oxidase (GOX), Lysozyme (LYS), Alkaline phosphatase (ALP) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). The human serum samples were provided by Xi'an No. 1 Hospital (Shanxi, China). Dulbecco's modi ed Eagle's medium (DMEM) was purchased from Sigma Aldrich Trading Co., Ltd (Shanghai, China). Milli-Q-puri ed water was applied for sample preparation.
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