Bm 20alite prominence hplc system

SMA-PTX micelles were solubilized at 1 mg/mL in methanol, and the loading was measured by high-performance liquid chromatography (HPLC) using a BM-20Alite Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan) on a reversed-phase Gemini 3 μm C18, 110A, 150×2 mm column (Phenomenex Gemini-NX) with a 2×4 mm C18 guard column. The mobile phase was acetonitrile and 0.03 M phosphate buffer (pH 3; 1:1 ratio). The flow rate was 1 mL/min with an injection volume of 10 μL, and the column temperature was set at 30°C. PTX peaks were detected at 226 nm with the retention time of 8.5 minutes.

The release of the payloads from the NPs was measured using a semipermeable dialysis membrane (14 kDa molecular weight cutoff, Sigma-Aldrich Co.) as detailed before.15 (link) The concentration of tDodSNO in the solutions was measured using a BM-20Alite Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan) on a reversed-phase Gemini 3 µm C18, 110A, 150×2 mm column (Phenomenex Gemini-NX, North Shore City, Auckland, New Zealand) at 25°C. The mobile phase was methanol containing 0.3% trifluoroacetic acid, and the flow rate was 0.65 mL/min. A diode array detector was set at 341 nm and linked to Chromeleon software for data analysis. tDodSNO peak was detected at 341 nm with the retention time of 4.3 minutes.