Antiphospho tbk1 antibody

Manufactured by Cell Signaling Technology

Antiphospho-TBK1 antibody is a laboratory reagent that detects the phosphorylated form of the Tank-binding kinase 1 (TBK1) protein. TBK1 is a serine/threonine protein kinase involved in various cellular signaling pathways. This antibody can be used to monitor the phosphorylation state of TBK1 in biological samples.

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Lab products found in correlation

Anti tbk1 antibody, by Cell Signaling Technology (2 mentions) Ecl kit, by GE Healthcare (1 mentions) Anti γh2ax antibody, by Merck Group (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Anti p65 antibody, by Cell Signaling Technology (1 mentions) Ecl prime, by GE Healthcare (1 mentions) Anti phospho p65 antibody, by Cell Signaling Technology (1 mentions) Anti phospho irf3 ser396 antibody, by Cell Signaling Technology (1 mentions) Anti sting antibody, by Cell Signaling Technology (1 mentions) Anti irf3 antibody, by Cell Signaling Technology (1 mentions) Anti phospho chk1 ser296 antibody, by Cell Signaling Technology (1 mentions) Anti chk1 antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using antiphospho tbk1 antibody

Immunoprecipitation and Immunoblotting of Phospho-TBK1

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Cells were lysed with RIPA buffer [50 mM Tris–HCl, pH 8.0, 150 mM NaCl, 0.1% (v/v) sodium dodecyl sulfate, 1% (v/v) Nonidet-P40, and 0.04% (v/v) sodium deoxycholate] and immunoprecipitated with an anti-TBK1 antibody (Cell Signaling Technology, Danvers, MA). Precipitates were blotted using an antiphospho-TBK1 antibody (Cell Signaling Technology). After washing, primary antibodies were detected with horseradish peroxidase (HRP)-conjugated antirabbit or antimouse secondary antibodies and an ECL kit (GE Healthcare, Chicago, IL). Images of uncropped blots are provided in Supplemental data 1–3.

Hiroki H., Ishii Y., Piao J., Namikawa Y., Masutani M., Honda H., Akahane K., Inukai T., Morio T, & Takagi M. (2023). Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells. Scientific Reports, 13, 7588.

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Immunoblotting of DNA Damage Signaling

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Cells were lyzed with NETN lysis buffer (150 mM NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 0.5% NP-40, 1 mM Na₃VO₄, 10 mM NaF, and protease inhibitor cocktail), followed by sonication for 15 seconds. After centrifugation, supernatant was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane. The following antibodies were used: anti-ZMYND8 antibody (A302–089A, Bethyl Laboratories), anti-cGAS antibody (31659S, Cell Signaling Technology), anti-STING antibody (13647S, Cell Signaling Technology), anti-phospho-TBK1 antibody (5483S, Cell Signaling Technology), anti-TBK1 antibody (3013S, Cell Signaling Technology), anti-phospho-IRF3 (Ser396) antibody (29047S, Cell Signaling Technology), anti-IRF3 antibody (4302S, Cell Signaling Technology), anti-phospho-p65 antibody (3033S, Cell Signaling Technology), anti-p65 antibody (8242S, Cell Signaling Technology), anti-phospho-Chk1 (Ser296) antibody (2349S, Cell Signaling Technology), anti-Chk1 antibody (sc-8408, Santa Cruz Biotechnology), anti-γH2A.X antibody (05–636, Sigma), anti-actin antibody (A2066, Sigma), and anti-H2A.X antibody (10856–1-AP, Proteintech). Proteins were visualized by chemiluminescence with ECL prime (GE Healthcare).

Wang Y., Luo M., Chen Y., Wang Y., Zhang B., Ren Z., Bao L., Wang Y., Wang J.E., Fu Y.X., Luo W, & Wang Y. (2020). ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth. Cancer research, 81(1), 174-186.

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