All the experiments were approved by the ethical committee from the university and conform with regulatory standards (LA1230406, project 666N). Mice were bred and maintained under pathogen-free conditions in a certified animal facility in accordance with the European guidelines. Adult (8-week-old) wild-type CD1 mice or Krt8-YFP knock-in mice (18 (link)) were used for sequencing and histology. Krt5-CreERT2 knock-in mice (the Jackson Laboratory, stock no. 029155; RRID:IMSR_JAX:029155) were crossed with R26R-EYFP (the Jackson Laboratory, stock no. 006148; RRID:IMSR_JAX:006148) to generate Krt5-CreERT2:R26R-EYFP (K5:RYFP) mice. For lineage tracing experiments, 10 or 0.25 mg of TAM resuspended in sunflower oil was injected intraperitoneally to 8-week-old K5:RYFP mice, and the expression of the YFP was analyzed in esophagi up to 4 weeks after TAM administration. In all the experiments, littermates of the same sex were randomly assigned to experimental groups.
Krt5 creert2 knock in mice
Manufactured by Jackson ImmunoResearch
Krt5-CreERT2 knock-in mice are genetically modified mice that express a tamoxifen-inducible Cre recombinase under the control of the Krt5 promoter. The Krt5 gene encodes the keratin 5 protein, which is expressed in basal epithelial cells. The Cre-ERT2 fusion protein allows for temporal control of Cre-mediated recombination upon administration of tamoxifen.
Automatically generated - may contain errors
2 protocols using krt5 creert2 knock in mice
1
Krt5-CreERT2:R26R-EYFP Lineage Tracing
2
Transgenic Mouse Models for Studying MYC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B6 (CD45.2) and B6-Ly5.1 (CD45.1) mice were purchased from Jackson Laboratory. The MycTg mice25 (link), in which a loxP-flanked ‘stop’ cassette precedes a human MYC cDNA inserted into the Rosa-26 locus, were purchased from Jackson Laboratory (Stock No: 020458) and crossed with FoxN1Cre mice that were a gift from G.Hollander24 (link). Myc-GFP mice45 (link), with Myc fused to GFP in the N-terminus, were purchased from Jackson Laboratory (Stock No: 21935).
Krt5-CreERT2 knock-in mice31 (link) were purchased from Jackson Laboratory (Stock No: 029155). Rag2-EGFP mice46 (link) were purchased from Jackson Laboratory (Stock No: 005688). Mice described as newborn were 0–2 days in age and of either sex. Mice described as adult for flow cytometry/sequencing experiments were 4–6 weeks of age and of either sex (unless otherwise stated). Aged mice were between 18 and 21 months of age. The ages of embryonic mice are specified with E0.5 being noon of the day of the discovered plug. Animal procedures were approved by relevant National Institutes of Health Animal Care and Use Committees.
Krt5-CreERT2 knock-in mice31 (link) were purchased from Jackson Laboratory (Stock No: 029155). Rag2-EGFP mice46 (link) were purchased from Jackson Laboratory (Stock No: 005688). Mice described as newborn were 0–2 days in age and of either sex. Mice described as adult for flow cytometry/sequencing experiments were 4–6 weeks of age and of either sex (unless otherwise stated). Aged mice were between 18 and 21 months of age. The ages of embryonic mice are specified with E0.5 being noon of the day of the discovered plug. Animal procedures were approved by relevant National Institutes of Health Animal Care and Use Committees.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!