Kolliphor HS15 was obtained from BASF, and Lipoid S75-3 was acquired from Lipoid GmbH (Ludwigshafen, Germany). Miglyol 812 [medium chain triglycerides (MCT)] was purchased from Caesar & Loretz GmbH (Hilden, Germany). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was purchased from Invitrogen (Waltham, USA). Sodium oleate was purchased from TCI Europe (Zwijindrecht, Belgium). CsA was obtained from Pharma Stulln GmbH (Stulln, Germany). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N[maleimide(polyethyleneglycol)-2000] (ammonium salt) (DSPE-PEG2000-maleimide) was purchased from Avanti Polar Lipids Inc. (Alabaster, USA). cRGD acetate salt was purchased from Bachem Distribution Service GmbH (Weil a. Rhein, Germany). Dulbecco’s PBS (DPBS) was acquired from Gibco Life Technologies (Waltham, USA). Purified water was obtained from a MilliQ System from Millipore (Schwalbach, Germany). All other materials and reagents in analytical grade were obtained from Merck (Taufkirchen, Germany).
S75 3
Manufactured by Lipoid
Sourced in Germany, France
Lipoid S75-3 is a phospholipid-based laboratory product. It is composed of phosphatidylcholine and related phospholipids derived from soybean. The product is designed for use in various research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Kolliphor hs 15, by BASF (5 mentions)
Milli q system, by Merck Group (3 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n maleimide polyethylene glycol 2000 ammonium salt dspe peg 2000 maleimide, by Avanti Polar Lipids (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Thermo Fisher Scientific (1 mentions)
Dulbecco s pbs dpbs, by Thermo Fisher Scientific (1 mentions)
Sodium oleate, by Tokyo Chemical Industry (1 mentions)
Chloroform, by Merck Group (1 mentions)
Labrafac cc, by Gattefosse (1 mentions)
Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions)
Milliq185 system, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Gel loading buffer, by Merck Group (1 mentions)
Solutol hs15, by Lipoid (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
9 protocols using s75 3
1
Preparation and Characterization of Nanoparticles
2
Lipoid-based Nanoemulsion Formulation
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Lipoid S75-3 (hydrogenated lecithin from soybean) was kindly provided by Lipoid Gmbh (Germany). Kolliphor HS 15 (Solutol HS 15, macrogol 15 hydroxystearate, polyoxyl 15 hydroxystearate; CAS Number: 70142-34-6; molecular weight: 963.24 g/mol) was kindly provided by BASF (Germany). Labrafac CC (caprylic/capric acid triglycerides) was kindly provided by Gattefossé S.A. (France). Sodium chloride, chloroform and all other chemicals and solvents were purchased from Sigma Aldrich (France).
3
Formulation of Lipid Nanocarrier Emulsions
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LNCs were formulated as described previously,32 (link) by combining 20.5% w/w Labrafac WL 1349 (caprylic-capric acid triglycerides, Gatefossé S.A. Saint-Priest, France), 1.5% w/w Lipoid S75-3 (Lipoid GmbH, Ludwigshafen, Germany), 16.9% w/w Kolliphor® HS 15 (BASF, Ludwigshafen, Germany), 1.8% w/w NaCl (Prolabo, Fontenay-sous-Bois, France), and 59.8% w/w water (obtained from a Milli-Q system, Millipore, Paris, France) with magnetic stirring. Three temperature cycles between 60 and 95 °C were performed to obtain phase inversions of the emulsion. Subsequent rapid cooling and dilution with ice-cooled water at the last phase inversion temperature (PIT) led to formation of blank LNCs.
4
Lipid Nanocapsule Formulation and Characterization
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Lipid nanocapsules were formulated as described [14 (link)] by mixing under magnetic stirring 20.2% w/w caprylic-capric acid triglycerides (Labrafac WL 1349, Gatefossé S.A., France), 17.2% w/w hydroxystearate-PEG (Kolliphor® HS 15, BASF, Ludwigshafen, Germany), 8% w/w NaCl (Prolabo, France), 1.5% w/w lecithin (Lipoid S75-3, Lipoid GmbH, Ludwigshafen, Germany) and 35.58% of Milli-Q water. Three cycles of heating and cooling between 60 and 90 °C were carried out to obtain the phase inversion. Ice-cold Milli-Q water 23.72% w/w was added at the last inversion temperature leading to the formation of lipid nanocapsules.
5
Preparation and Characterization of Cyclosporin A Lipid Nanoparticles
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Cyclosporin A (CsA) was obtained from Pharma Stulln GmbH (Stulln, Germany). Kolliphor® HS15 was obtained from BASF. Lipoid® S75-3 was obtained from Lipoid GmbH (Ludwigshafen, Germany). Miglyol® 812 (MCT) was purchased from Caesar & Loretz GmbH (Hilden, Germany). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N[maleimide(polyethyleneglycol)-2000] (ammonium salt) (DSPE-PEG2000-maleimide) was purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and cyclo(-Arg-Gly-Asp-D-Phe-Cys) acetate salt (RGD) from Bachem Distribution Service GmbH (Weil a. Rhein, Germany). Dulbecco’s phosphate-buffered saline (PBS) was acquired from Gibco® Life Technologies (Waltham, USA). Purified water was obtained from a Milli-Q System from Millipore (Schwalbach, Germany). All other materials and reagents in analytical grade were obtained from Merck (Taufkirchen, Germany).
6
Formulation and Characterization of Bedaquiline-Loaded Nanocapsules
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Reagents required for formulation were sourced from the same suppliers as previously described [22 (link)]. Tween 20 and absolute EtOH, were purchased from Panreac Química S.L.U (Barcelona, Spain). Span 85 (sorbitanetrioleate), oleic acid and chitosan (medium molecular weight) were purchased from Sigma-Aldrich Pte. Ltd. (Singapore). Bedaquiline was obtained from AURUM Pharmatech LLC (Franklin Park, NJ, USA). Bis(sulfosuccinimidyl) suberate (BS3) was purchased from Pierce Biotechnology Inc. (Rockford, IL, USA) and α-methoxy-ω-amino poly(ethylene glycol) (PEG-MW 5000 Dalton) from IRIS Biotech GmbH (Marktredwitz, Germany). Myrj S40 (PEG 40 stearate, 1980 Da) and Super Refined Soybean Oil were obtained from Croda Uniquema (Chocques, France). Suppocire NB was purchased from Gattefosse S.A. (Saint-Priest, France). Lipoid S75-3 (soybean lecithin at 69% of phosphatidylcholine) and hydrogenated S75 were provided by Lipoid GmbH (Ludwigshafen, Germany). All the products were pharmaceutical grade and used as received. DOTAP (1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)) was purchased from Avanti Polar Lipids, Inc. (Alabaster, Alabama, USA). Water (double processed tissue culture, endotoxin-free) used in all nanocapsule synthesis was from Sigma-Aldrich.
7
Formulation and Characterization of SFN Nanoparticles
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SFN powder was purchased from LC Laboratories (Wobern, USA). Oil solubilizers and excipients were provided by Gattefosse S.A (Saint-Priest, France). Lipoid® S75-3 (soybean lecithin with 70% phosphatidylcholine and 10% phosphatidylethanolamine) and Kolliphor® HS15 (mixture of free polyethylene glycol 660 and polyethylene glycol 660 hydroxystearate) were gifts from Lipoid Gmbh (Ludwigshafen, Germany) and BASF (Ludwigshafen, Germany), respectively. NaCl was purchased from Prolabo VWR International (Fontenay-sous-Bois, France). Purified water was obtained with a MilliQ185 System (Millipore, Paris, France). Formic acid, acetic acid, acetonitrile, dimethylsulfoxide (DMSO), methanol, and HPLC-grade tetrahydrofurane (THF) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France) and Carlo Erba reagents (Val-de-Reuil, France).
8
Formulation and Characterization of Taxol Nanoparticles
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Lipoid® S75-3 (soybean lecithin at 69% of phosphatidylcholine), Captex® 8000 (glyceryltricaprylate) and Solutol® HS15 (polyethyleneglycol ester of 12-hydroxystearic acid and polyethylene glycol-PEG-SA) were obtained from Lipoid GmbH (Ludwigshafen, Germany), Abitec Corp (Colombus, OH, USA) and BASF (Ludwigshafen, Germany), respectively. Ethanol Solution 96% was purchased from Fisher Scientific (Illkirch, France). Taxol® 6mg/ml solution and PTX powder were supplied by Bristol-Myers Squibb (Rueil-Malmaison, France) and LC Laboratories® (Woburn, MA 01801, USA), respectively. Sodium chloride, sodium dodecyl sulphate (SDS), chitosan low molecular weight (average Mn 5,000 Da, > 90% deacetylated), propidium iodide, bis-chloroethylnitrosourea (BCNU) and gel loading buffer were purchased from Sigma Aldrich (Saint-Quentin-Fallavier, France). CpG-ODN: Phosphorothioated-CpG-oligodeoxynucleotide 1826, seq (5'-3': tccatgacgttcctgacgtt) (CpG) was purchased from Invivogen (Toulouse, France). Annexin V-FITC was purchased from BD Pharmingen (San Diego, CA, USA).
9
Formulation Development of Lipid-Based Dosage
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ABZ powder was purchased to from Parafarm (Buenos Aires, Argentina) and Captex 8000 ® (tricaprylin) was supplied by Abitec Corp. (Colombus, Ohio, USA). The lipophilic Labrafac ® WL 1349 (caprylic-capric acid triglycerides; European Pharmacopeia, IVth, 2002) and Transcutol HP ® (diethylene glycol monoethyl ether) were kindly provided by Gattefossé S.A.S (Saint-Priest, France).
Lipoid ® S75-3 (soybean lecithin at with 70% of phosphatidylcholine and 10% of phosphatidylethanolamine) and Koliphor ® HS-15 (mixture of free polyethylene glycol 660 and polyethylene glycol 660 hydroxystearate) were gifts from Lipoid Gmbh (Ludwigshafen, Germany) and BASF (Ludwigshafen, Germany), respectively. Due to the complex composition of each product, the brand names will be used from here on in the following text. NaCl was purchased from the Cicarelli lab (Buenos Aires, Argentina), and oleic acid (OA), monobasic ammonium phosphate, polisorbate 80 (Tween 80 ® ) and Carboxymethilcellulose (CMC) were purchased from Sintorgan (Buenos Aires, Argentina). Porcine pepsin and pancreatin (reagent grade) enzymes were American Chemical Society (ACS) and were bought from Sigma (St. Louis, MO, USA). Purified water was obtained from MilliQ System (Biopore, Buenos Aires, Argentina). All other chemical reagents were of HPLC-grade and acquired from Sintorgan, Argentina.
Lipoid ® S75-3 (soybean lecithin at with 70% of phosphatidylcholine and 10% of phosphatidylethanolamine) and Koliphor ® HS-15 (mixture of free polyethylene glycol 660 and polyethylene glycol 660 hydroxystearate) were gifts from Lipoid Gmbh (Ludwigshafen, Germany) and BASF (Ludwigshafen, Germany), respectively. Due to the complex composition of each product, the brand names will be used from here on in the following text. NaCl was purchased from the Cicarelli lab (Buenos Aires, Argentina), and oleic acid (OA), monobasic ammonium phosphate, polisorbate 80 (Tween 80 ® ) and Carboxymethilcellulose (CMC) were purchased from Sintorgan (Buenos Aires, Argentina). Porcine pepsin and pancreatin (reagent grade) enzymes were American Chemical Society (ACS) and were bought from Sigma (St. Louis, MO, USA). Purified water was obtained from MilliQ System (Biopore, Buenos Aires, Argentina). All other chemical reagents were of HPLC-grade and acquired from Sintorgan, Argentina.
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