Ple gal4

All flies were raised at 25°C in a 12:12 h light:dark cycle. Flies were fed standard cornmeal-dextrose agar media. CASK proteins (full-length wild type β isoform or mutants containing a deletion of either the L27 or CaMK domains) tagged with a C-terminal YFP were expressed in a cell-specific manner using the GAL4/UAS system (Brand and Perrimon, 1993 (link)). All experiments were done on a CASK-β null genetic background [CASK^P18 allele (Slawson et al., 2011 (link))] to ensure that the only CASK-β present was the product of the tagged transgene. Briefly, animals carrying a UAS-CASK-YFP transgene (made from a cDNA encoding isoform B) were crossed to animals carrying GAL4's which expressed in specific cell types. The GAL4's used in this study were: C155-GAL4 (Genotype:P{GawB}elavC155, Bloomington stock 43351; chromosome X), all neurons (Lin et al., 1994 (link)); C164-GAL4 (Genotype:P{GawB}C164, Bloomington stock 33807), a subset of adult neurons including some dopaminergic neurons (Slawson et al., 2011 (link); Slawson et al., in preparation); TH-GAL4, (Genotype:P{ple-GAL4.F}, Bloomington stock 8848; Chromosome 3), dopaminergic neurons (Friggi-Grelin et al., 2003 (link)); and DIlp2-GAL4 (Genotype:P{Ilp2-GAL4.R}, Bloomington stock 37516; Chromosome 2), neurons of the pars intercerebralis which express Drosophila insulin-like peptide 2 (Corl et al., 2004 (link)).

ple-GAL4 (BDSC8848, RRID:BDSC_8848), VGlut^OK371-GAL4 (BDSC26160, RRID:BDSC_26160), ChAT^7.4-GAL4 (BDSC56500, RRID:BDSC_56500), tdc2-GAL4 (BDSC9313, RRID:BDSC_9313), dilp2-GAL4 (BDSC37516, RRID:BDSC_37516), and HMS01544 (BDSC36129, RRID:BDSC_36129) were from Bloomington Drosophila Stock Center. elav-GAL4 was a gift from J. Treisman. PLA2G6 null mutants and isogenic controls were generated in the Steinhauer lab (J.S., in preparation).
Drosophila were cultured on standard media containing 3.83% molasses, 1.58% yeast, and 3.83% corn meal, supplemented with 0.11% methyl paraben and 0.38% propionic acid as mold inhibitors.