Penicillin streptomycin

Manufactured by Quality Biological

Sourced in United States

Penicillin/streptomycin is a common antibiotic solution used in cell culture media. It contains the antibiotics penicillin and streptomycin, which prevent bacterial growth and contamination in cell culture experiments.

Automatically generated - may contain errors

Lab products found in correlation

L glutamine, by Quality Biological (13 mentions) Non essential amino acids (neaa), by Merck Group (5 mentions) Hepes, by Quality Biological (5 mentions) Fetal bovine serum (fbs), by Quality Biological (4 mentions) Rpmi 1640, by Quality Biological (3 mentions) Sodium pyruvate, by Quality Biological (3 mentions) 2 3 butanedione monoxime, by Merck Group (3 mentions) Protease xiv, by Merck Group (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Hek293t cell, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by GE Healthcare (2 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions) Collagenase 2, by Worthington (2 mentions) Abcg2b, by GenScript (2 mentions) Abcg2c, by GenScript (2 mentions) Abcg2d, by GenScript (2 mentions) Hek293 cell, by American Type Culture Collection (2 mentions)

Spelling variants of this product

Streptomycin (36 citations) Penicillin (34 citations) Amphotericin B/penicillin/streptomycin (2 citations)

29 protocols using penicillin streptomycin

Cell Culture Protocols for Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

U937, CEM, and Jurkat cells were obtained from ATCC (Manassas, VA) and maintained in Roswell Park Memorial Institute (RPMI) 1640 media containing 10% heat-inactivated fetal bovine serum (FBS), 1% l-glutamine, and 1% streptomycin/penicillin (Quality Biological, Gaithersburg, MD). 293T cells, VP40 clones, and HeLa cells were sustained in Dulbecco's modified Eagle medium (DMEM) containing 10% heat-inactivated fetal bovine serum (FBS), 1% l-glutamine, and 1% streptomycin/penicillin (Quality Biological). Peripheral blood mononuclear cells (PBMCs) were maintained in RPMI 1640 media containing 20% FBS, 1% l-glutamine, and 1% streptomycin/penicillin. The PBMCs were also maintained with 50 IU/mL human recombinant interleukin (IL)-2 (Sigma) before transfections, but they were incubated in the absence of exogenous growth factors once transfected. All cells were incubated at 37°C with 5% CO₂. For antibiotic selection of transfected 293T cells, hygromycin B (Invitrogen) was added to the culture the next day. Cdk4/6 inhibitors Fascaplysin (0.1–1 µM; Abcam) and Ribociclib (LEEO11; 0.1–10.0 µM; MedChemExpress) were used for the treatment of 293T and VP40 clone cell cultures for the analysis of cyclin D1 inhibition, cell viability, and EV production.

Pleet M.L., Erickson J., DeMarino C., Barclay R.A., Cowen M., Lepene B., Liang J., Kuhn J.H., Prugar L., Stonier S.W., Dye J.M., Zhou W., Liotta L.A., Aman M.J, & Kashanchi F. (2018). Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles. The Journal of Infectious Diseases, 218(Suppl 5), S365-S387.

+ Open protocol

+ Expand

Culturing HIV-1 Infected T-cells and Luciferase Reporter Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The uninfected Jurkat and HIV-1 infected J1.1 T-cells obtained from the NIH AIDS Research and Reference Reagent Program, were maintained in RPMI-1640 media containing 10% heat-inactivated fetal bovine serum (FBS), 1% L-glutamine, and 1% streptomycin/penicillin (Quality Biological, Gaithersburg, MD). A HeLa cell derivative TZM-bl cells, expressing CD4, CXCR4 and CCR5 surface receptors, and with a stably integrated firefly luciferase reporter gene under the control of an HIV-1 LTR [43] (link), [44] (link), was maintained in DMEM supplemented with 10% FBS, 1% L-glutamine, and 1% streptomycin/penicillin. All cells were incubated at 37°C in the presence of 5% CO₂.

Jaworski E., Saifuddin M., Sampey G., Shafagati N., Van Duyne R., Iordanskiy S., Kehn-Hall K., Liotta L., Petricoin E I.I.I., Young M., Lepene B, & Kashanchi F. (2014). The Use of Nanotrap Particles Technology in Capturing HIV-1 Virions and Viral Proteins from Infected Cells. PLoS ONE, 9(5), e96778.

+ Open protocol

+ Expand

Caco-2 Cell Culturing and Seeding

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caco-2 cell line was purchased from American Type Culture Collection (ATCC# HTB-37), and culturing was performed as described in published best practices from the ATCC manual (https://www.atcc.org/Products/All/HTB-37.aspx?geo_country=us), and as reported in literature.^12,22 Cryovials were thawed and cultured in 12 mL media in T75 flasks. Cells were grown to 60–80% confluency, media was aspirated, rinsed with HBSS, harvested with 3 mL .25% Trypsin-EDTA and split 1–2 or 1–3. Viable cell counts were obtained using Nexcelom Bioscience Cellomenter™ Auto T4 cell counter with Trypan Blue assay for viability (BioWhittaker® catalog# 17-942E). Cells were initially thawed from cryopreserved vials with 5 or 6 previous passages and seeded after 3–4 additional passages.
After expansion, cells were seeded onto 24-well insert plates with PET 1.0 uM pore size permeable membrane (Millipore product # PSRP010). Cells were seeded at a density of .4x10⁶ cells/mL in a 300uL volume (120,000 cells/well) in the apical chamber. Cultures were grown at 37°C, 5% CO2 on filter-sterilized Eagle’s Minimum Essential Medium (EMEM) with 20% Fetal Bovine Serum, with Penicillin-Streptomycin (Quality Biological™ cat. # 120–095-671). Media was changed every other day during the timecourse.

Robinson J.M., Turkington S., Abey S.A., Kenea N, & Henderson W.A. (2019). Differential gene expression and gene-set enrichment analysis in Caco-2 monolayers during a 30-day timeline with Dexamethasone exposure: A data modeling approach to understanding culture age as co-variate for differential expression in a non-renewing epithelial monolayer using a gene ontology-defined 250-plex Nanostring probe panel.. Tissue Barriers, 7(3), e1651597.

+ Open protocol

+ Expand

Expansion of HIV/EBV-specific CD8+ T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thawed PBMCs from HIV-infected individuals were labeled with CellTrace Violet (Thermo Fisher Scientific) at 1μM according to the manufacturer's protocol. The cells were then plated at 5 × 10⁶ cells/well in 24 well plate in CellGenix GMP DC Medium (CellGenix) containing recombinant human (rh) IL-7 (1ng/ml, PeproTech) to support minimal T cell survival and rh FLT3L (50ng/ml, PeproTech) to mobilize primary DCs. After 24 hours (day 1), cognate CD8 epitope peptides from HIV or EBV were added to the culture at 1μg/ml. On day 2, human serum (Access Biologicals) and rh IL-2 (Miltenyi Biotec) were added at final concentrations of 8% (volume/volume) and 20U/ml, respectively. Half of the medium was replaced with RPMI-1640 containing 8% human serum, rhIL-2 (20U/ml), and penicillin-streptomycin (100 U/ml, Quality Biological) on days 5, 7 and 9. HIV and EBV-specific CD8⁺ T cells in the culture were analyzed by flow cytometry on days 6 and 12.

Takata H., Kakazu J.C., Mitchell J.L., Kroon E., Colby D.J., Sacdalan C., Bai H., Ehrenberg P.K., Geretz A., Buranapraditkun S., Pinyakorn S., Intasan J., Tipsuk S., Suttichom D., Prueksakaew P., Chalermchai T., Chomchey N., Phanuphak N., de Souza M., Michael N.L., Robb M.L., Haddad E.K., Crowell T.A., Vasan S., Valcour V.G., Douek D.C., Thomas R., Rolland M., Chomont N., Ananworanich J, & Trautmann L. (2022). Long-term antiretroviral therapy initiated in acute HIV infection prevents residual dysfunction of HIV-specific CD8+ T cells. eBioMedicine, 84, 104253.

+ Open protocol

+ Expand

Isolation and Culture of Cardiac ILC2s

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiac ILC2s were isolated using FACSAria II (BD Biosciences) from the hearts of IL-33-treated mice and cultured at 37°C in RPMI 1640 (GIBCO) with 10% FBS (GE Healthcare Life Sciences), 1% Penicillin-Streptomycin, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium Pyruvate (all Quality Biological), 0.1 mM Non-essential amino acids (Sigma Aldrich), 0.05 mM 2-Mercaptoethanol (GIBCO). Cytokines, IL-2, IL-7 and IL-33, at a concentration of 25 ng/ml were added in media to expand ILC2s in vitro.

Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.

+ Open protocol

+ Expand

Synthesis and Characterization of (R,R')-MNF Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

(R,R’)-MNF was synthesized as previously described.^{14 (link)} Lactate (purity ≥ 98%), 3-hydroxybutyrate (purity ≥ 98%), carnitine (purity ≥ 98%), p-aminohippuric acid (purity ≥ 98%), human recombinant insulin (dry powder), methanol (HPLC grade), acetonitrile (HPLC grade) and formic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 medium, Eagle’s Minimum Essential Medium (EMEM), trypsin solution, phosphate-buffered saline (PBS), fetal bovine serum (FBS), 100X solutions of sodium pyruvate (100 mM), L-glutamine (200 mM), and penicillin/streptomycin (a mixture of 10,000 units/mL) were obtained from Quality Biological (Gaithersburg, MD, USA).

Bernier M., Catazaro J., Singh N.S., Wnorowski A., Boguszewska-Czubara A., Jozwiak K., Powers R, & Wainer I.W. (2017). GPR55 receptor antagonist decreases glycolytic activity in PANC-1 pancreatic cancer cell line and tumor xenografts. International journal of cancer, 141(10), 2131-2142.

+ Open protocol

+ Expand

Isolation and Primary Culture of Mouse Dorsal Root Ganglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult (8–10 weeks) male naive mice were euthanized with isoflurane. DRGs were collected in cold DH10 [90%DMEM/F-12 (Gibco, Grand Island, NY), 10% FBS (JR Scientific, Woodland, CA), 1%penicillin-streptomycin (Quality Biological, Gaithersburg, MD)] and then treated with enzyme solution [3.5 mg/ml dispase, 1.6 mg/ml collagenase type I in HBSS without Ca2+and Mg2+ (Gibco)] at 37°C for 20–25 min. After centrifugation, the dissociated cells were re-suspended in DH10 and plated at a density of 1.5–4×10⁵ cells in a 6-well plate coated with poly-L-lysine (0.5 mg/ml, Sigma, St. Louis, MO) and laminin (10 μg/ml, Invitrogen). The cells were incubated in 5% CO2 at 37°C. One day later, 500 ng siRNA was added to each well. Cells were harvested 48 hours later.

Wen J., Yang Y., Wu S., Wei G., Jia S., Hannaford S, & Tao Y.X. (2020). Long noncoding RNA H19 in the injured dorsal root ganglion contributes to peripheral nerve injury-induced pain hypersensitivity. Translational perioperative and pain medicine, 7(2), 176-184.

+ Open protocol

+ Expand

Synthesis and Use of Radiolabeled MNF

Check if the same lab product or an alternative is used in the 5 most similar protocols

(R,R’)-4-methoxy-1-naphthylfenoterol (MNF) was synthesized as described previously [24 (link)]. Radiolabeled [³H]-thymidine (10 Ci/mmol) was purchased from PerkinElmer Life Science (Waltham, MA); human recombinant insulin, AM251, doxorubicin, gemcitabine, and CID 16020046 were from Sigma-Aldrich (St-Louis, MO), while the Nuclear and Cytoplasmic Extraction Reagents (NE-PER Kit) was obtained from Thermo Fisher Scientific (Waltham, MA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 medium, Eagle's Minimum Essential Medium (EMEM), trypsin solution, phosphate-buffered saline (PBS), fetal bovine serum (FBS), 100X solutions of sodium pyruvate (100 mM), L-glutamine (200 mM), and penicillin/streptomycin (a mixture of 10,000 units/ml penicillin and 10,000 μg/ml streptomycin) were obtained from Quality Biological (Gaithersburg, MD).

Singh N.S., Bernier M, & Wainer I.W. (2016). Selective GPR55 antagonism reduces chemoresistance in cancer cells. Pharmacological research, 111, 757-766.

+ Open protocol

+ Expand

Cannabidiol and HU308 Effects on HIV-1 Monocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

HIV-1-infected (U1) monocytes provided by the National Institutes of Health’s (NIH) AIDS Reagent program were cultured in RPMI 1640 media containing 10% EV-depleted fetal bovine serum (FBS), 1% penicillin/streptomycin, and 1% L-glutamine (Quality Biological, Gaithersburg, MD, USA). Cells were incubated at 37 °C in 5% CO₂. U1 cells were treated with Cannabidiol (CBD; 2-[1R-3-methyl-6R-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-1,3-benzenediol; CAT: 90080, Cayman Chemical, Ann Arbor, MI, USA; 5 µM) or HU308 (((1S,4S,5S)-4-(2,6-dimethoxy-4-(2-methyloctan-2-yl)phenyl)-6,6-dimethylbicyclo [3.1.1]hept-2-en-2-yl)methanol; Tetrabiopharma and Targetedbioscience); 5 µM) for 3 days.

Williams A., Khatkar P., Branscome H., Kim Y., Erickson J., Jenabian M.A., Costiniuk C.T, & Kashanchi F. (2023). The Use of CBD and Its Synthetic Analog HU308 in HIV-1-Infected Myeloid Cells. Pharmaceuticals, 16(8), 1147.

+ Open protocol

+ Expand

Murine Bone Marrow-Derived Macrophage Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow was extracted from femurs and tibias isolated from BALB/cJ or C57BL/6J mice and cultured in DMEM media (12-604F, Lonza, Basel, Switzerland) with 10% heat-inactivated fetal bovine serum (35-011-CV, Corning Inc., Corning, NY) and 1% penicillin/streptomycin (120-095-721EA, Quality Biological, Gaithersburg, MD) at 37°C, 5% CO₂, in a humidified incubator. After 24 h, the suspension cells were removed and culture in a new flask along with 10 ng/ml recombinant murine IL-3 (213-13, Peprotech, Rocky Hill, NJ). Cells were passaged twice per week, each time leaving behind any adherent cells. After 4–6 weeks, cell phenotype was confirmed by visual morphology and flow cytometry.

Strattan E., Palaniyandi S., Kumari R., Du J., Hakim N., Huang T., Kesler M.V., Jennings C.D., Sturgill J.L, & Hildebrandt G.C. (2019). Mast Cells Are Mediators of Fibrosis and Effector Cell Recruitment in Dermal Chronic Graft-vs.-Host Disease. Frontiers in Immunology, 10, 2470.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!