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Eia kit

Manufactured by R&D Systems
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EIA kits are laboratory tools used for enzyme-linked immunosorbent assay (ELISA) testing. These kits provide the necessary reagents and materials to perform quantitative or qualitative analysis of specific proteins, hormones, or other analytes in biological samples.

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Lab products found in correlation

8 isoprostane eia kit, by Cayman Chemical (1 mentions) Nitrate nitrite fluorometric assay kit, by Cayman Chemical (1 mentions) Cyclic gmp eia kit, by Cayman Chemical (1 mentions) Endpoint genotyping software, by Roche (1 mentions) Lightcycler 480 real time system, by Roche (1 mentions) Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions) Cocktail of protease inhibitor, by Roche (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Pepstatin, by Merck Group (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Leupeptin, by Merck Group (1 mentions) Silymarin, by Merck Group (1 mentions) Hacat, by CLS Cell Lines Service (1 mentions) Eia kit, by Cayman Chemical (1 mentions) Bradford assay, by Bio-Rad (1 mentions) Optimax, by Molecular Devices (1 mentions) Prostaglandin d2 mox eia kit, by Cayman Chemical (1 mentions) Pro prep buffer, by iNtRON Biotechnology (1 mentions) Protein assay reagent, by Bio-Rad (1 mentions)

31 protocols using eia kit

1

Measurement of Inflammatory Mediators in BALF

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Interferon (IFN)-γ, interleukin (IL)-13, IL-5, IL-6, keratinocyte-derived chemokine (KC/CXCL1), and macrophage inflammatory protein (MIP)-2 (KC/CXCL1 and MIP-2/CXCL2 are murine homologues of human IL-8) levels in BALF were measured using enzyme immunoassay (EIA) kits (R&D Systems Europe, Abingdon, UK). Granulocyte-macrophage colony stimulating factor (GM-CSF) levels in serum and BALF were measured using EIA kits (R&D Systems Europe), as were CysLTs and LTB4 levels (Cayman Chemical, Ann Arbor, MI, USA). BALF dilutions were 1:5 for IL-13, IL-5, IL-6, KC/CXCL1, and MIP-2/CXCL2. BALF dilutions were 1:2 for CysLTs and LTB4. BALF was undiluted for IFN-γ. Serum was undiluted for GM-CSF. All EIA assays were performed according to the manufacturer’s instructions.
Kurai J., Watanabe M., Sano H., Hantan D., Tohda Y, & Shimizu E. (2016). Effects of Asian Dust Particles on the Early-Stage Antigen-Induced Immune Response of Asthma in NC/Nga Mice. International Journal of Environmental Research and Public Health, 13(11), 1144.
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2

Measuring Nitric Oxide and PGE2 in RAW 264.7 Cells

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NO levels in the culture media were determined using the Greiss reaction assay as described previously [34 (link)]. Briefly, RAW 264.7 cells (1 × 104 cells/well) were seeded in a 96-well culture plate. Cells were treated with LPS in the presence or absence of various doses of GTEO (25–100 μg/mL) for 24 h. After treatment, an equal volume of culture supernatants was mixed with Griess reagent and incubated at room temperature for 30 min. The intercellular level of nitrate, a major stable product of NO, was measured with an ELISA microplate reader at 540 nm (µQuant, Bio-Tek Instruments, Winooski, VA, USA). On the other hand, intercellular PGE2 levels were determined using an EIA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocols.
Lin W.T., He Y.H., Lo Y.H., Chiang Y.T., Wang S.Y., Bezirganoglu I, & Kumar K.J. (2023). Essential Oil from Glossogyne tenuifolia Inhibits Lipopolysaccharide-Induced Inflammation-Associated Genes in Macro-Phage Cells via Suppression of NF-κB Signaling Pathway. Plants, 12(6), 1241.
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3

Platelets Thromboxane B2 and cAMP Assay

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Platelet suspensions (1 × 108 platelets/ml) were pre-incubated with lunathrombase (0.2 µM) for 5 min and thereafter 2 mM EDTA and 50 µl indomethacin (1 mM) were added to the suspensions. The thromboxane B2 level (TxB2) and cAMP levels of the supernatants were measured by an enzyme immuno assay (EIA) kit (R&D systems, USA) following the instructions of the manufacturer. The cAMP level in the platelet suspensions incubated with 0.2 µM PGE1 (positive control) was also measured under identical conditions. The cyclooxygenase-1 (COX-1) inhibitory effect of lunathrombase/ibuprofen/aspirin (0.2 µM) was determined using the commercial kit (R&D systems, USA) following the instructions of the manufacturer.
Gogoi D., Arora N., Kalita B., Sarma R., Islam T., Ghosh S.S., Devi R, & Mukherjee A.K. (2018). Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica. Scientific Reports, 8, 6210.
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4

Cytokine and Oxidative Stress Biomarkers

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The RIF collections were immediately stored at −80°C until assayed. RIF TNF-α and IL-6 were measured using an EIA kit (R&D Systems, Minneapolis, MN, USA). RIF NO recovery levels were measured using a nitrate/nitrite fluorometric assay kit (Cayman) and presented as µmol/min. RIF cGMP recovery levels were measured using a cyclic GMP EIA kit (Cayman). RIF 8-isoprostane recovery levels were measured using a 8-isoprostane EIA kit (Cayman). Both RIF cGMP and RIF 8-isoprostane are presented as fmol/min.
Matavelli L.C., Zatz R, & Siragy H.M. (2015). A NONPEPTIDE ANGIOTENSIN II TYPE 2 RECEPTOR AGONIST PREVENTS RENAL INFLAMMATION IN EARLY DIABETES. Journal of cardiovascular pharmacology, 65(4), 371-376.
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5

Lupane modulates cytokine production in PBMCs

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PBMCs (2 × 105/well) in an RPMI assay medium {RPMI-1640 medium supplemented with 100 μg/mL of gentamicin, 2 mM of L-glutamine and 10 % FBS) were incubated with lupane (0.3, 0.7 and 1.5 μg/mL) or without for 15 h at 37 °C, 5 % CO2. PBMCs were incubated with RPMI for the negative control, and with LPS (1 μg/mL) for the positive control. After incubation, plates were centrifuged at 405 xg for 5 min and the supernatants were collected for cytokine detection. The cytokines TNF-α and IL-10 were quantified using an enzyme immunoassay (EIA) kit (R&D Systems) according to the manufacturer’s instructions.
Lacouth-Silva F., Xavier C.V., da S. Setúbal S., Pontes A.S., Nery N.M., de Castro O.B., Fernandes C.F., Honda E.R., Zanchi F.B., Calderon L.A., Stábeli R.G., Soares A.M., Silva-Jardim I., Facundo V.A, & Zuliani J.P. (2015). The effect of 3β, 6β, 16β-trihydroxylup-20(29)-ene lupane compound isolated from Combretum leprosum Mart. on peripheral blood mononuclear cells. BMC Complementary and Alternative Medicine, 15, 420.
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6

IFNL3 and IFNL4 Genotyping and Protein Assay

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Genotyping of IFNL3 single-nucleotide polymorphism rs12979860 and the IFNL4-ΔG SNP, rs368234815, were conducted using custom TaqMan® SNP Genotyping Assays from Applied Biosystems (Life Technologies, Carlsbad, CA, USA) performed according to manufacturer’s instructions. The Roche LightCycler 480 Real-Time System (Roche Applied Science, Indianapolis, IN, USA) was used to amplify the DNA and measure the fluorescence from each reaction to determine each genotype as calculated by Endpoint Genotyping Software (Roche, Nutley, NJ, USA).
Human IFNL protein levels have been challenging to measure and discriminate between the different subtypes: human IFNL1/IFNL3 levels were measured using a commercially available EIA kit (R&D Systems Inc., Minneapolis, MN, USA; Cat. number DY1598B).
Kurbanov F., Kim Y., Latanich R., Chaudhari P., El-Diwany R., Knabel M., Kandathil A.J., Cameron A., Cox A., Jang Y.Y., Thomas D.L, & Balagopal A. (2015). IFNL3 genotype is associated with differential induction of IFNL3 in primary human hepatocytes. Antiviral therapy, 20(8), 805-814.
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7

Quantifying Inflammatory Cytokines in Serum

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Serum levels of the pro-inflammatory cytokine, TNF-α, were determined by the quantitative enzyme immunoassay (EIA) kit purchased from R&D Systems (USA) according to the method of Howard and Harada69 , while that of anti-inflammatory cytokine IL-10 was according to Croft et al.70 (link).
Ezz Eldin R.R., Saleh M.A., Alotaibi M.H., Alsuair R.K., Alzahrani Y.A., Alshehri F.A., Mohamed A.F., Hafez S.M., Althoqapy A.A., Khirala S.K., Amin M.M., A. F Y., AbdElwahab A.H., Alesawy M.S., Elmaaty A.A, & Al-Karmalawy A.A. (2022). Ligand-based design and synthesis of N'-Benzylidene-3,4-dimethoxybenzohydrazide derivatives as potential antimicrobial agents; evaluation by in vitro, in vivo, and in silico approaches with SAR studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 1098-1119.
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8

Quantification of Neurochemicals in Rat Spinal Cord

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Rat spinal cord (L4, 5 lumbar enlargement) was collected after behavioral testing and frozen immediately on dry ice. The spinal cord tissue was cut into fine pieces in ice-cold PBS, pH 7.4, containing a cocktail of protease inhibitors (Roche Applied Science) followed by homogenization using a Dounce tissue grinder. Homogenates were centrifuged for 15 min at 12,000 g, 4℃. The supernatants were aliquoted and stored at −80℃ until required for enzyme immunoassay (EIA) performance. Total protein contents in all tissue extracts were measured using the DC protein assay kit (Bio-Rad). Brain-derived neurotrophic factor (BDNF), prodynorphin (PDYN), and CXCL1 levels were determined in duplicate using respective EIA kits, according to the manufacturer’s instructions. BDNF EIA kit was from LifeSpan BioSciences. PDYN concentration was measured using the PDYN EIA kit (antibodies-online). CXCL1 level was determined with an EIA kit from R&D Systems. These assay systems detect rat BDNF, PDYN, and CXCL1 with sensitivity of 12.29 pg/ml, 3.9 pg/ml, and 1.3 pg/ml, respectively. The concentrations of BNDF, PDYN, and CXCL1 proteins were calculated from the standard curve at each assay. Each protein concentration was expressed as pg/mg total protein.
Liang D.Y., Shi X., Liu P., Sun Y., Sahbaie P., Li W.W., Yeomans D.C, & Clark J.D. (2017). The chemokine receptor CXCR2 supports nociceptive sensitization after traumatic brain injury. Molecular Pain, 13, 1744806917730212.
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9

Corticosterone Serum Level Measurement

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The corticosterone assay has been described previously [25 (link)]. Corticosterone serum levels (n = 7–9 per group) were measured using a commercially available enzyme immunoassay (EIA) kit (R&D Systems, Inc., Minneapolis, MN, USA), according to the manufacturer's instructions.
Lee M.S., Kim Y.H., Lee B.R., Kwon S.H., Moon W.J., Hong K.S., Song Y.S., Morita K., Hahm D.H., Shim I, & Her S. (2014). Novel Antidepressant-Like Activity of Caffeic Acid Phenethyl Ester Is Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 646039.
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10

Quantifying Galectin-3 Levels

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The level of galectin-3 in the cell culture supernatant was determined using a commercially available enzyme immunoassay (EIA) kit (R&D Systems) according to the manufacturer's instructions. The catalog numbers for human galectin-3 and mouse galectin-3 were DGAL30 and DY1197, respectively. Each measurement was performed in triplicate, and the average value was then recorded as ng/mL.
Lin Y.H., Chou C.H., Wu X.M., Chang Y.Y., Hung C.S., Chen Y.H., Tzeng Y.L., Wu V.C., Ho Y.L., Hsieh F.J, & Wu K.D. (2014). Aldosterone Induced Galectin-3 Secretion In Vitro and In Vivo: From Cells to Humans. PLoS ONE, 9(9), e95254.
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