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Facscan analysis

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The FACScan is a flow cytometry instrument designed for quantitative analysis of cells and particles. It utilizes a laser-based detection system to measure various physical and fluorescent properties of individual cells or particles as they flow through a fluidic system. The FACScan is capable of analyzing multiple parameters simultaneously, providing a comprehensive analysis of cellular characteristics.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (1 mentions) Modfit cell cycle analysis software, by BD (1 mentions) Propidium iodide, by Merck Group (1 mentions) Pi staining solution, by Beyotime (1 mentions) Modfit lt software, by Verity Software House (1 mentions) Anti gr1, by BioLegend (1 mentions) Anti cd11c, by BioLegend (1 mentions) Anti f4 80, by BD (1 mentions) Anti cd11b, by BD (1 mentions) Cd11b, by BD (1 mentions) F4 80, by BD (1 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Cytochalasin b, by Merck Group (1 mentions) Cyto id autophagy detection kit, by Enzo Life Sciences (1 mentions) Mtt assay kit, by Abcam (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)

Close spelling variants of this product

FACScan (3082 citations) FACScan/CellQuest analysis (1 citations)

6 protocols using facscan analysis

1

Cell Cycle Analysis of Bladder Cancer Cells

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Parental T24, T24-Ad- CADM1, T24-Ad-sh1 and T24-Ad-GFP1/2 cells were harvested and single-cell suspensions were prepared by passing the cells through a nylon mesh. Subsequently, cells were fixed in 70% ethanol at −20°C for 2 h, washed 3 times with ice-cold PBS and suspended in propidium iodide (PI) buffer (Invitrogen; Thermo Fisher Scientific, Inc.) with cells adjusted to a density of 4×106/ml. The cells were then incubated with 5 µg/ml propidium iodide (Sigma-Aldrich; Merck KGaA) at room temperature for 30 min in the dark. The single cells were then subjected to FACScan analysis (Becton-Dickinson, Heidelberg, Germany). Cell cycle distribution was calculated using ModFIT cell cycle analysis software (version 2.01.2; Becton Dickinson). Each experiment was repeated at least three times on different days for validation.
Chen Y., Liu L., Guo Z., Wang Y., Yang Y, & Liu X. (2018). Lost expression of cell adhesion molecule 1 is associated with bladder cancer progression and recurrence and its overexpression inhibited tumor cell malignant behaviors. Oncology Letters, 17(2), 2047-2056.
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2

Cell Cycle Analysis After gRB1 Modulation

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After overexpression or knockdown of gRB1 for 48 h, ICP1 cells were trypsinized and subsequently fixed with ice-cold 70% ethanol for at least 1 h. After extensive washing, the cells were suspended in propidium iodide (PI) staining solution (Beyotime), fully resuspended slowly, and bathed at 37°C for 30 min in the dark for subsequent FACScan analysis (Becton-Dickinson, San Jose, CA, USA). Cell-cycle analysis was performed by the ModFit LT software (Verity Software House, Topsham, ME, USA). The experiments were repeated biologically three times. Each biological repeat included three wells technical duplications. Only the result of one biological repeat was shown in the current study, because the results of the three biological repeats were similar with each other.
Wang Y., Wang H., Na W., Qin F., Zhang Z., Dong J., Li H, & Zhang H. (2019). The retinoblastoma 1 gene (RB1) modulates the proliferation of chicken preadipocytes. British poultry science, 60(3).
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3

Peritoneal Immune Cell Profiling in CLP

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Peritoneal lavage was harvested from mice at different time points before and after CLP. Peritoneal exudate cells were incubated with anti-CD11b (BD Pharmingen), anti-CD11c (BioLegend), anti-F4/80 (BD Pharmingen), and anti-Gr1 (BioLegend) monoclonal antibodies (mAbs) conjugated with FITC, PE, PerCP, or APC. FITC-, PE-, PerCP-, and APC-conjugated anti-mouse isotype-matched mAbs (BD Pharmingen; BioLegend) were used as negative controls. Subpopulations of macrophages (CD11b+F4/80−CD11clo) and PMNs (CD11b+F4/80−Gr1hi) in the peritoneal cavity were detected by FACScan analysis (BD Biosciences).
Huang J., Ita M., Zhou H., Zhao H., Hassan F., Bai Z., O’Leary D.P., Li Y., Redmond H.P., Wang J.H, & Wang J. (2022). Autophagy induced by taurolidine protects against polymicrobial sepsis by promoting both host resistance and disease tolerance. Proceedings of the National Academy of Sciences of the United States of America, 119(19), e2121244119.
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4

Assaying Bacterial Phagocytosis and Killing

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Bacterial phagocytosis and intracellular bacterial killing were determined as described previously (45 (link), 47 (link)). Briefly, S. aureus and S. typhimurium were heat killed and labeled with 0.1% FITC (Sigma-Aldrich). Isolated macrophages were pretreated with PBS, 3-MA, or DPI; then incubated with CM or taurolidine; and further challenged with heat-killed, FITC-labeled S. aureus and S. typhimurium for 30 min to assess bacterial phagocytosis or live S. aureus and S. typhimurium for 60 and 120 min in the presence or absence of cytochalasin B (5 µg/mL) (Sigma-Aldrich) to assess bacterial killing. Bacterial phagocytosis was assessed by FACScan analysis (BD Biosciences). Intracellular bacterial killing was calculated based on the total and extracellular bacterial killings.
Huang J., Ita M., Zhou H., Zhao H., Hassan F., Bai Z., O’Leary D.P., Li Y., Redmond H.P., Wang J.H, & Wang J. (2022). Autophagy induced by taurolidine protects against polymicrobial sepsis by promoting both host resistance and disease tolerance. Proceedings of the National Academy of Sciences of the United States of America, 119(19), e2121244119.
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5

Autophagy Induction in Immune Cells

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Isolated peritoneal macrophages or BMMs, primary PECs, and IECs were incubated with CM or taurolidine at the indicated concentrations for various time periods. Autophagy induction was assessed either by FACScan analysis (BD Biosciences) or by a Synergy HTX multimode plate reader (BioTec) after live cells were stained using the Cyto-ID autophagy detection kit (Enzo Life Sciences) according to the manufacturer’s instruction.
Huang J., Ita M., Zhou H., Zhao H., Hassan F., Bai Z., O’Leary D.P., Li Y., Redmond H.P., Wang J.H, & Wang J. (2022). Autophagy induced by taurolidine protects against polymicrobial sepsis by promoting both host resistance and disease tolerance. Proceedings of the National Academy of Sciences of the United States of America, 119(19), e2121244119.
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6

Evaluating Apoptosis and Viability

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Isolated primary PECs and IECs were pretreated with PBS or 3-MA, then incubated with CM or taurolidine, and further challenged with LPS (2.0 µg/mL) for 24 h. Cell apoptosis in PECs was assessed by FACScan analysis (BD Biosciences) using a FITC Annexin V apoptosis detection kit (BD Pharmingen). Cell viability in IECs was spectrophotometrically determined using a MTT assay kit (Abcam).
Huang J., Ita M., Zhou H., Zhao H., Hassan F., Bai Z., O’Leary D.P., Li Y., Redmond H.P., Wang J.H, & Wang J. (2022). Autophagy induced by taurolidine protects against polymicrobial sepsis by promoting both host resistance and disease tolerance. Proceedings of the National Academy of Sciences of the United States of America, 119(19), e2121244119.
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