Transmission electronic microscopy (TEM) was conducted on JEM-2100 F transmission electronic microscope (JEOL, Japan). Scanning electronic microscopy (SEM) and scanning transmission electron microscopy (STEM) observations were performed on Magellan 400 scanning electronic microscope (FEI, USA). X-ray diffraction (XRD) patterns were obtained by using Rigaku D/Max-2550V diffractometer (Geigerflex, Japan). X-ray photoelectron spectroscopy (XPS) was conducted on ESCAlab250 (Thermo Fisher Scientific, USA). Specific surface area of the sample was determined by using Micromeritics ASAP 2010 analyzer (Micromeritics, USA). Fourier transform infrared spectroscopy (FTIR) was recorded on a spectrometer (Nicolet iS50, USA). The oxygen concentration in the solution was determined by a dissolved oxygen analyzer (JPBJ-609L, REX, China). The glucose content in medium was measured by glucose test strips (Sinocare, China). The wavelength of near infrared (NIR) laser was 808 nm (PM100D, Thorlabs GmbH, Germany). The temperature changes of the samples were recorded via an infrared-thermal imaging camera (A325sc, FLIR, USA). The released concentrations of Si and Mn elements in the solution were detected by inductively coupled plasma atomic emission spectrometry (ICP-AES, Vista AX, USA).
Glucose test strips
Manufactured by Sinocare
Sourced in China
Glucose test strips are a type of lab equipment used for the analysis of glucose levels in biological samples. They function by reacting with glucose in the sample, producing a measurable signal that can be interpreted to determine the glucose concentration.
Automatically generated - may contain errors
3 protocols using glucose test strips
1
Comprehensive Materials Characterization Protocol
2
Glucose Monitoring Protocol Using Sannuo+ Glucometer
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The personal glucometer (Sannuo+) and glucose test strips were purchased from Sinocare Inc. (Changsha, China). A DHG-9035A drying oven and HWS-12 thermostatic water bath were purchased from Shanghai Yiheng Technology Instrument Co., Ltd. (Shanghai, China). The vortex mixer XW-80A type was purchased from Kylin-Bell Lab Instruments Co., Ltd. (Ningbo, China). The SB-4200DT ultrasonic cleaner was purchased from Ningbo Scientz Biotechnology Co., Ltd. (Ningbo, China).
3
Leptin-Deficient Mice on High-Fat Diet
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C57BL mice (20 ± 2 g) and leptin-deficient mice (OB) used in the present study were obtained from the experimental animal center of Gannan Medical College (Ganzhou, China). The animals were maintained at a controlled temperature (22°C ± 1°C), humidity (50%), and light (12-h light/dark). All animal experiments were approved by the experimental animal ethics committee of Gannan Medical College. A HFD consists of 20% carbohydrates, 35% protein, and 45% fat (according to D12492; Research Diets Inc.; 60% of total calories). The SSO drug was obtained from MCE (Hy-112847) and was fed at a dose of 50 mg/kg/day. Blood glucose level was measured using equipment and glucose test strips provided by Sinocare.
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