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Anti snrnp70

Manufactured by Abcam
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Anti-SNRNP70 is a laboratory product that recognizes the SNRNP70 protein. SNRNP70 is a component of the small nuclear ribonucleoprotein complex involved in pre-mRNA splicing. This product can be used for detection and research purposes.

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Lab products found in correlation

Normal mouse igg, by Abcam (4 mentions) Ago2 antibody, by Abcam (4 mentions) Thermo fisher rip kit, by Thermo Fisher Scientific (3 mentions) Anti histone h3, by Abcam (2 mentions) Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Anti kdm7a, by GeneTex (1 mentions) Anti h3k4me3, by Merck Group (1 mentions) Anti h3k27me2, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti tubulin, by Abcam (1 mentions) Anti h3k4me2, by Merck Group (1 mentions) Anti h3k4me1, by Merck Group (1 mentions) Anti hnrnp a1, by Merck Group (1 mentions) Anti h3k27me1, by Abcam (1 mentions) Anti h3k79me2, by Abcam (1 mentions) Anti kdm4a, by Abcam (1 mentions) Anti kdm5a, by Abcam (1 mentions) Anti kdm5c, by Abcam (1 mentions) Anti u2af2, by Abcam (1 mentions)

6 protocols using anti snrnp70

1

Quantifying XIST Expression via RIP

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RNA immunoprecipitation (RIP) experiment was conducted using Magna RIP RNA‐binding protein immunoprecipitation kit (Millipore, Billerica, MA, USA) and the Ago2 antibody (Abcam, Cambridge, MA, USA) according to the manufacturer's instruction. The expression of XIST was analysed by qRT‐PCR. Normal mouse IgG (Abcam, USA) and anti‐SNRNP70 (Abcam, USA) were, respectively, used as negative control and positive control.
Zhang Y., Zhu Y., Gao G, & Zhou Z. (2019). Knockdown XIST alleviates LPS‐induced WI‐38 cell apoptosis and inflammation injury via targeting miR‐370‐3p/TLR4 in acute pneumonia. Cell Biochemistry and Function, 37(5), 348-358.
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2

Antibody Validation for Epigenetic Analysis

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Antibodies were purchased from the following companies: anti‐H3K27me1, anti‐H3K27me3, anti‐H3K79me1, anti‐H3K79me2, anti‐histone H3, anti‐hnRNP I, anti‐KDM4A, anti‐KDM5A, anti‐KDM5C, anti‐snRNP70, anti‐tubulin, and anti‐U2AF2 from Abcam (Cambridge, MA, USA); anti‐hnRNP A2/B1 from Acris (San Diego, CA, USA); anti‐SR protein‐specific kinases (SRPK)1 and anti‐SRPK2 from BD Biosciences (San Diego, CA, USA); anti‐AKT, anti‐H3K27me2, anti‐protein phosphatase‐1 (PP1), anti‐phospho‐PP1 at Thr320, anti‐phospho‐AKT at Ser473, and anti‐cleaved‐CASPASE‐3 from Cell Signaling Technology (Billerica, MA, USA); anti‐H3K4me1, anti‐H3K4me2, anti‐H3K4me3, and anti‐H3K36me3 from Millipore; anti‐pro‐CASPASE‐3 and anti‐KDM7A from GeneTex (SanAntonio, TX, USA); anti‐hnRNP C1/C2, anti‐BAX, and anti‐BCL2L1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti‐hnRNP A1 from Sigma; and anti‐SRSF1 and anti‐SRSF3 from Zymed (San Francisco, CA, USA).
Lee C., Chang W., Chang Y., Yang J., Chang C., Hsu K., Chen Y., Liu T., Chen Y., Lin S., Wu Y, & Chang J. (2019). Alternative splicing in human cancer cells is modulated by the amiloride derivative 3,5‐diamino‐6‐chloro‐N‐(N‐(2,6‐dichlorobenzoyl)carbamimidoyl)pyrazine‐2‐carboxide. Molecular Oncology, 13(8), 1744-1762.
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3

Ago2 RNA Immunoprecipitation Protocol

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RNA immunoprecipitation was performed using thermo fisher RIP kit (Thermo, USA) based on the manufacturer's protocol. The Ago2 antibodies were purchased from Abcam (USA). Normal mouse IgG (Abcam, USA) was applied as negative control and anti- SNRNP70 (Abcam, USA) was employed as positive control for the RIP procedure. Purified RNA was subjected to qRT-PCR analysis to demonstrate the presence of the binding targets using respective primers.
Pan Y., Chen J., Tao L., Zhang K., Wang R., Chu X, & Chen L. (2017). Long noncoding RNA ROR regulates chemoresistance in docetaxel-resistant lung adenocarcinoma cells via epithelial mesenchymal transition pathway. Oncotarget, 8(20), 33144-33158.
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4

Ago2 RNA Immunoprecipitation Protocol

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RNA immunoprecipitation was performed using thermo fisher RIP kit (Thermo, USA) based on the manufacturer’s protocol. The Ago2 antibodies are purchased from Abcam (USA). Normal mouse IgG (Abcam, USA) was applied as negative control and anti-SNRNP70 (Abcam, USA) was employed as positive control for the RIP procedure. Purified RNA was subjected to qRT-PCR analysis to demonstrate the presence of the binding targets using respective primers.
Zhang K., Chen J., Song H, & Chen L.B. (2017). SNHG16/miR-140-5p axis promotes esophagus cancer cell proliferation, migration and EMT formation through regulating ZEB1. Oncotarget, 9(1), 1028-1040.
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5

Ago2 Protein-RNA Immunoprecipitation Assay

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RNA immunoprecipitation was performed using thermo fisher RIP kit (Thermo, USA) based on the manufacturer's protocol. The Ago2 antibodies were purchased from Abcam (USA). Normal mouse IgG (Abcam, USA) was applied as negative control and anti-SNRNP70 (Abcam, USA) was employed as positive control for the RIP procedure. Purified RNA was subjected to qRT-PCR analysis to demonstrate the presence of the binding targets using respective primers.
Chen J., Zhang K., Song H., Wang R., Chu X, & Chen L. (2016). Long noncoding RNA CCAT1 acts as an oncogene and promotes chemoresistance in docetaxel-resistant lung adenocarcinoma cells. Oncotarget, 7(38), 62474-62489.
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6

SUMO2 Antibody Production and Validation

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SUMO hybridoma SUMO2 8A2, were procured from Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology (http://dshb.biology.uiowa.edu/) and supernatant was produced in Pichler lab. The SUMO2 antisera were used at 1:100. All other primary antibodies were purchased and validated by the manufacturers (data available on manufacturers’ websites). They were used at the indicated dilutions; anti-NELFA (goat polyclonal, sc-23599 (A-20), Santa Cruz Biotechnology) at 1:1,000, anti-NELFC/D (mouse monoclonal, sc-393972 (C-10), Santa Cruz Biotechnology) 1:1,000, anti-NELFE (rabbit polyclonal, sc-32912 (H-140), Santa Cruz Biotechnology) at 1:1,000, anti-SNRNP70 (rabbit polyclonal, ab83306, Abcam) at 1:1,000, anti-ZNF451 (rabbit polyclonal, A305-177A, Bethyl Laboratories inc), anti-RPB3 (rabbit polyclonal, ABE999, Millipore) at 1:1,000, and anti-Histone H3 (rabbit polyclonal, ab70550, Abcam) at 1:5,000. Secondary antibodies against goat, rabbit or mouse proteins were horseradish peroxidase conjugated and were typically used at 1:10,000 dilution. Anti-rabbit (NA934V) and anti-mouse (NA931V) antibodies were purchased from GE healthcare. Anti-goat (sc-2354) antibody was purchased from Santa Cruz Biotechnology.
Rawat P., Boehning M., Hummel B., Aprile-Garcia F., Pandit A.S., Eisenhardt N., Khavaran A., Niskanen E., Vos S.M., Palvimo J.J., Pichler A., Cramer P, & Sawarkar R. (2021). Stress-induced nuclear condensation of NELF drives transcriptional downregulation. Molecular Cell, 81(5), 1013-1026.e11.
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