Ab124707

Manufactured by Abcam

Sourced in United Kingdom

Ab124707 is a primary antibody product manufactured by Abcam. It is a mouse monoclonal antibody that recognizes a specific target protein.

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Lab products found in correlation

2 protocols using ab124707

Comprehensive Immunoblotting Methodology

Cited 1 time

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Immunoblotting was performed as described previously (35 (link)). The following antibodies were used at a concentration of 0.1 to 0.5 μg/ml: anti-pMCM2 (3378-1; Epitomics Inc.), anti-MCM2 (sc-9839; Santa Cruz Biotechnology), anti–pPOL II (04-1571; Millipore), anti–POL II (05-952; Millipore), anti-Lamin B1 (ab16048; Abcam), anti-FANCD2 (sc-20022; Santa Cruz Biotechnology), anti-pCDC6 (ab76422; Abcam), anti-CDC6 (ab109315; Abcam), pChk1 (#2348; Cell Signaling Technology), anti-Chk1 (#2345; Cell Signaling Technology), anti-pCDK1 (#9111; Cell Signaling Technology), anti-CDK1 (sc-954; Santa Cruz Biotechnology), anti-gH2AX (#2577; Cell Signaling Technology), anti-PARP1 (#9542; Cell Signaling Technology), anti–cyclin B1 (sc-752; Santa Cruz Biotechnology), anti-CDC7 (sc-56274; Santa Cruz Biotechnology), anti-DBF4 (ab124707; Abcam), and anti-GAPDH (MAB374; Chemicon). Immunoblotted proteins were visualized by chemiluminescence.

Iwai K., Nambu T., Dairiki R., Ohori M., Yu J., Burke K., Gotou M., Yamamoto Y., Ebara S., Shibata S., Hibino R., Nishizawa S., Miyazaki T., Homma M., Oguro Y., Imada T., Cho N., Uchiyama N., Kogame A., Takeuchi T., Kurasawa O., Yamanaka K., Niu H, & Ohashi A. (2019). Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Science Advances, 5(5), eaav3660.

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Western Blot Analysis of DBF4 and CDC7

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Fresh frozen tissues from HCC patients were collected and lysed with RIPA buffer according to the manufacturer’s instructions (Beyotime Biotechnology, Shanghai, China). For each lane, 50 μg protein was loaded for detecting DBF4 and CDC7 on different gels. Proteins were separated with SDS-PAGE and transferred to 0.45 μm PVDF membranes. Membranes were then blocked with PBS containing 0.05% tween and 5% non-fat milk. DBF4 antibody (ab124707, Abcam, Cambridge, UK) and CDC7 antibody (ab229187, Abcam, Cambridge, UK) were used to detect the protein level of DBF4 and CDC7, and GAPDH (ET1601-4, HuaBio, Hangzhou, China) was used as an internal control.

Wang M., Qiu Z.H., Wang Y.Z., Lian B., Bai J.K., Zhou Y.J, & Ji H.J. (2023). Analysis of the expression and prognostic significance of DDK complex in Hepatocarcinoma. BMC Cancer, 23, 19.

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