6 mv linear accelerator

Manufactured by Siemens

Sourced in United States, Germany

The 6 MV Siemens linear accelerator is a medical device designed for the precise delivery of radiation therapy. It generates high-energy X-rays that can be directed at specific targets within the body. The device is capable of producing a beam of electrons or photons with an energy of up to 6 million electron volts (MV).

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Lab products found in correlation

Eca 109, by Procell (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) β ionizing radiation, by Siemens (1 mentions) Phytohemagglutinin (pha), by Harvard Bioscience (1 mentions) Colcemid, by Thermo Fisher Scientific (1 mentions)

22 protocols using 6 mv linear accelerator

Irradiation of Cell Samples

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Irradiation of the samples was performed at room temperature using a 6 MV Siemens linear accelerator (Siemens, Concord, CA) at a dose rate of 2 Gy min^− 1. After exposure to IR, cells were further cultured in CGM for the indicated time until processing [24 (link)].

Djuzenova C.S., Fiedler V., Memmel S., Katzer A., Sisario D., Brosch P.K., Göhrung A., Frister S., Zimmermann H., Flentje M, & Sukhorukov V.L. (2019). Differential effects of the Akt inhibitor MK-2206 on migration and radiation sensitivity of glioblastoma cells. BMC Cancer, 19, 299.

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Radiation Exposure Procedure for Cells

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Irradiation was performed at room temperature using a 6 MV Siemens linear accelerator (Siemens, Concord, CA) at a dose rate of 2 Gy/min. After irradiation, cells were kept in CGM for the indicated time until harvest.

Djuzenova C.S., Fischer T., Katzer A., Sisario D., Korsa T., Steussloff G., Sukhorukov V.L, & Flentje M. (2021). Opposite effects of the triple target (DNA-PK/PI3K/mTOR) inhibitor PI-103 on the radiation sensitivity of glioblastoma cell lines proficient and deficient in DNA-PKcs. BMC Cancer, 21, 1201.

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Radiation Exposure Protocol for Cells

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Djuzenova C.S., Fiedler V., Katzer A., Michel K., Deckert S., Zimmermann H., Sukhorukov V.L, & Flentje M. (2016). Dual PI3K- and mTOR-inhibitor PI-103 can either enhance or reduce the radiosensitizing effect of the Hsp90 inhibitor NVP-AUY922 in tumor cells: The role of drug-irradiation schedule. Oncotarget, 7(25), 38191-38209.

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ESCC Cell Line Radiation Exposure

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Human normal esophageal epithelial cells (HEEC) and Human ESCC cell lines, including ECA109, TE1, KYSE30, KYSE150, KYSE410, and KYSE450, were obtained from the Research Center of the Fourth Hospital of Hebei Medical University (Shijiazhuang, China). Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin at 37 °C incubator containing 5% CO². The medium of cell lines was refreshed every 2 to 3 days. ESCC cells were exposed to X-ray irradiation by adopting the single energy 6-MV Siemens linear accelerator (Siemens, Buffalo Grove, IL, USA) at a 3 Gy/min dose-rate.

Guo D., Zhou S., Liu R., Yao W., Li S., Zhang X., Shen W, & Zhu S. (2024). NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21. Cancer Cell International, 24, 179.

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X-irradiation Protocol using Siemens Accelerator

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X-irradiation was performed using a 6 MV Siemens linear accelerator (Siemens, Concord, CA, USA) with a dose rate of 2 Gy/min.

Meyer T.J., Scherzad A., Moratin H., Gehrke T.E., Killisperger J., Hagen R., Wohlleben G., Polat B., Dembski S., Kleinsasser N, & Hackenberg S. (2019). The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro. Materials, 12(24), 4062.

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Isolation and Irradiation of PBMCs

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The final cell density of isolated G0 unstimulated PBMCs was adjusted to 1 × 10⁶ cells/ml and the samples were placed at 37 °C in a 5 % CO₂ incubator. X-irradiation (0.5 and 2 Gy) was performed using a 6 MV Siemens linear accelerator (Siemens Concord, CA, USA) at a dose rate of 2 Gy/min. Non-irradiated cells were treated in similar way, but at a zero radiation dose.

Djuzenova C.S., Zimmermann M., Katzer A., Fiedler V., Distel L.V., Gasser M., Waaga-Gasser A.M., Flentje M, & Polat B. (2015). A prospective study on histone γ-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome. BMC Cancer, 15, 856.

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Radiation Exposure Protocol for Cells

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Irradiation was performed at room temperature using a 6 MV Siemens linear accelerator (Siemens, Concord, CA, USA) at a dose rate of 2 Gy/min. After irradiation, cells were kept in CGM for the indicated time until harvest.

Grabenbauer F., Katzer A., Sisario D., Memmel S., Flentje M., Sukhorukov V.L, & Djuzenova C.S. (2018). MEK-inhibitor PD184352 enhances the radiosensitizing effect of the Hsp90 inhibitor NVP-AUY922: the role of cell type and drug-irradiation schedule. Oncotarget, 9(100), 37379-37392.

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Siemens Linear Accelerator Irradiation

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Irradiation was performed at room temperature using a 6 MV Siemens linear accelerator (Siemens, Concord, CA) at a dose rate of 9.5 Gy/min.

Fischer T., Hartmann O., Reissland M., Prieto-Garcia C., Klann K., Pahor N., Schülein-Völk C., Baluapuri A., Polat B., Abazari A., Gerhard-Hartmann E., Kopp H.G., Essmann F., Rosenfeldt M., Münch C., Flentje M, & Diefenbacher M.E. (2022). PTEN mutant non-small cell lung cancer require ATM to suppress pro-apoptotic signalling and evade radiotherapy. Cell & Bioscience, 12, 50.

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Culturing and Irradiating Human ESCC Cells

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Human ESCC lines (KYSE150, TE1, KYSE450, KYSE410, KYSE30 and ECA109) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO₂. All cells were obtained from the Research Center of the Fourth Hospital of Hebei Medical University.
ESCC cells were IR by a 6-MV Siemens linear accelerator (Siemens, Buffalo Grove, IL, USA) at room temperature. The total dose was 6/8/10 Gy, and the dose rate was 300 MU/min. The source-skin distance (SSD) was 100 cm, and cells were then collected at specific times for further study.

Du X., Zhang X., Dong J., Zou N., Guo D., Yao W., Wang X., Li S., Song C., Yan K., Shen W, & Zhu S. (2022). Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC. Journal of Translational Medicine, 20, 507.

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FAM83D Regulates ESCC Radiosensitivity

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The human normal esophageal cell line HEEC was purchased from the Cellular Biology Institute of the Shanghai Academy of Sciences (Shanghai, China). The human ESCC cell lines ECA109 and TE1 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). EC9706, KYSE30 and KYSE180 cell lines were purchased from Otwo Biotech Inc. (Shenzhen, China). The above cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in 5% CO2. Plasmid containing the short hairpin RNA (shRNA) of FAM83D and the negative control (shNC) shRNA were purchased from GeneChem. Cells were seeded in 6-well plates and incubated for 24 h and transfected using Lipofectamine RNAiMAX (Invitrogen, USA) according to the manufacturer’s protocol. To obtain cells stably expressing FAM83D shRNA, G418 was added twenty-four hours after transfection, and stable transfectants were obtained after several weeks. Cells were irradiated at room temperature with a 6-MV Siemens linear accelerator (Siemens, Concord, CA) at a dose rate of 5 Gy/min after stable transfection and allowed to recover in an incubator for the indicated time until harvesting.

Yang X.X., Ma M., Sang M.X., Zhang X.Y., Zou N.Y, & Zhu S.C. (2020). Knockdown of FAM83D Enhances Radiosensitivity in Coordination with Irradiation by Inhibiting EMT via the Akt/GSK-3β/Snail Signaling Pathway in Human Esophageal Cancer Cells. OncoTargets and therapy, 13, 4665-4678.

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