Formvar film

Manufactured by Electron Microscopy Sciences

Sourced in Switzerland

Formvar film is a thin, transparent polymer film used as a support substrate for transmission electron microscopy (TEM) specimens. It provides a stable and uniform platform for the samples, enabling high-resolution imaging and analysis.

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Lab products found in correlation

Jem 1400, by JEOL (4 mentions) Jem 1400 tem, by JEOL (2 mentions) Orius sc1000 ccd camera, by Ametek (2 mentions) Jem 1200 exii electron microscope, by JEOL (1 mentions) Uranyless em stain, by Electron Microscopy Sciences (1 mentions) 1200ex electron microscope, by JEOL (1 mentions) S 3400n scanning electron microscope, by Hitachi (1 mentions) Ab64193, by Abcam (1 mentions) Superblock blocking buffer, by Thermo Fisher Scientific (1 mentions) Copper grid, by Electron Microscopy Sciences (1 mentions) Jem 2100 transmission electron microscope, by JEOL (1 mentions) Oneview, by Ametek (1 mentions) Jem 2100f, by Ametek (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Ammonium hydroxide, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Igepal co 520, by Merck Group (1 mentions) Tetraethyl orthosilicate, by Merck Group (1 mentions) 1 octadecene, by Merck Group (1 mentions) Jem 1011, by JEOL (1 mentions)

21 protocols using formvar film

Morphological Examination of PRG Carbopol 981 ME Eye Drops

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The morphology of our PRG Carbopol 981 ME eye drops was examined using transmission electron microscopy (TEM) (JEOL JEM1200EX II electron microscope). Briefly, the ME formulation was diluted 1:100 with Milli-Q water. Two microliters of the diluted ME was placed on 400 mesh copper grids covered with Formvar film (Electron Microscopy Sciences EMS, Hatfield, PA). The grids were allowed to dry for 2 h in a desiccator followed by negative staining with Uranyless EM stain (Electron Microscopy Sciences EMS, Hatfield, PA) before examination by TEM.

Ibrahim M.M., Maria D.N., Mishra S.R., Guragain D., Wang X, & Jablonski M.M. (2019). Once Daily Pregabalin Eye Drops for Management of Glaucoma. ACS nano, 13(12), 13728-13744.

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Negative Staining Electron Microscopy

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Samples (10 µL) were placed for 2 min on 400-mesh copper grids covered with carbon-stabilized Formvar film (Electron Microscopy Sciences (EMS), Hatfield, PA). Excess fluid was removed, and grids were negatively stained with 2% uranyl acetate solution (10 µL) for 2 min. After excess fluid removal, the samples were visualized using a JEM-1400 TEM (JEOL), operated at 80 kV.

Paul A., Frenkel-Pinter M., Escobar Alvarez D., Milordini G., Gazit E., Zacco E, & Segal D. (2020). Tryptophan-galactosylamine conjugates inhibit and disaggregate amyloid fibrils of Aβ42 and hIAPP peptides while reducing their toxicity. Communications Biology, 3, 484.

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Transmission Electron Microscopy Protocol

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TEM analysis was performed by applying 10 μL samples to 400-mesh copper grids covered by carbon-stabilized Formvar film (Electron Microscopy Science, Fort Washington, PA, USA). The samples were allowed to adsorb for 2 min before excess fluid was blotted off. For samples that were negatively stained, 10 μL of 2% uranyl acetate was then deposited on the grid and allowed to adsorb for 2 min before excess fluid was blotted off. TEM micrographs were recorded using JEOL 1200EX electron microscope (Tokyo, Japan) operating at 80 kV.

Fichman G., Adler-Abramovich L., Manohar S., Mironi-Harpaz I., Guterman T., Seliktar D., Messersmith P.B, & Gazit E. (2014). Seamless Metallic Coating and Surface Adhesion of Self-Assembled Bioinspired Nanostructures Based on Di-(3,4-dihydroxy-l-phenylalanine) Peptide Motif. ACS Nano, 8(7), 7220-7228.

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Electron Microscopy of ECM and Biofilm

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Negative staining transmission electron microscopy was performed on ECM samples that were applied to 300-mesh copper grids coated with Formvar film (Electron Microscopy Sciences, Hatfield, PA) for 2 min, rinsed in deionized water. The samples were negatively stained with 2% uranyl acetate for 90 s and then air-dried. Microscopy was performed on the JEM-1400 (JEOL, LLC). Scanning electron microscopy was performed on an intact bacterial biofilm gown on minimal nutrient agar medium, cut in an intact agar block and prepared as follows: fixed in 2% gluteraldehyde/4% formaldehyde in 0.1 M sodium cacodylate buffer (pH 7.3) in a petri dish for 45 minutes at 4 °C, washed and then postfixed with 1% osmium tetroxide in 0.1 M sodium cacodylate buffer for 45 minutes. The fixed biofilm sample was dehydrated in a series of increasing concentrations of ethanol (50%, 70%, 95%, and 100%), inserted into a critical point dryer (CPD) to remove residual ethanol with carbon dioxide, and then coated with gold-palladium and visualized with a Hitachi S-3400N scanning electron microscope.

Reichhardt C., Fong J.C., Yildiz F, & Cegelski L. (2014). Characterization of the Vibrio cholerae Extracellular Matrix: A Top-Down Solid-State NMR Approach. Biochimica et biophysica acta, 1848(1 0 0), 378-383.

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Immunogold Labeling of Tau Aggregates

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A 2 μL aliquot from the PNGase F-treated or untreated aggregated concentrated media was applied onto the dark side of 400-mesh copper grid covered with carbon-stabilized Formvar film (Electron Microscopy Sciences) and allowed to float for 2 min. Excess solution was removed using blotting paper and the grid was allowed to dry for 2 min^{28 (link)}. Then, the grid was blocked with SuperBlock blocking buffer (Thermo Scientific) for 30 min. Samples were incubated with the primary antibody recognizing total tau (ab64193, abcam, 1:100) in blocking buffer for 30 min, washed five times with the same buffer solution, and then incubated with secondary goat anti-rabbit antibody conjugated with 18-nm gold (111-215-144, Jackson ImmunoResearch, 1:20) for 30 min and similarly washed. Samples were viewed using a JEM-1400Plus electron microscope operating at 80 kV.

Losev Y., Paul A., Frenkel-Pinter M., Abu-Hussein M., Khalaila I., Gazit E, & Segal D. (2019). Novel model of secreted human tau protein reveals the impact of the abnormal N-glycosylation of tau on its aggregation propensity. Scientific Reports, 9, 2254.

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Negative Staining of Phage Particles

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Ten microliters of each highly purified phage (~10¹² PFU/mL) were fixed onto copper grids (Electron Microscopy Sciences) that were supported by carbon-coated Formvar film [58 (link)]. Phages were then negatively stained with 2% (w/v) aqueous phosphate tungsten acid, pH 7.2 for 1 min. and then air-dried for 1 h at room temperature. A JEOL JEM-2100 transmission electron microscope was used for acquiring the phage particle images at the Electron Microscope Facility, Al-Mansoura University, Egypt.

Esmael A., Azab E., Gobouri A.A., Nasr-Eldin M.A., Moustafa M.M., Mohamed S.A., Badr O.A, & Abdelatty A.M. (2021). Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods. Microorganisms, 9(2), 423.

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Peptide-Polydiacetylene Nanoparticle Analysis

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Images were obtained
using a JEOL JEM-2100F equipped with a Gatan K3 direct electron detector
and a Gatan OneView camera at an operating voltage of 80–200
kV. The samples were prepared using the same methods to prepare for
UV-vis and CD. The transmission electron microscopy (TEM) grids were
prepared by pipetting 5 μL of each peptide-PDA solution onto
200 mesh copper grids coated with a Formvar film (Electron Microscopy
Sciences) and 4 nm carbon coating and adsorbed for 30 s at room temperature.
The grid was washed with fresh Milli-Q water right away to prevent
the sample from drying, and the excess solution was removed by touching
the side of each grid with filter paper. The sample was then stained
with a 1% uranyl acetate solution for 30 s and washed with fresh Milli-Q
water, and excess moisture was removed.

Lim S., Cordova D.L., Robang A.S., Kuang Y., Ogura K.S., Paravastu A.K., Arguilla M.Q, & Ardoña H.A. (2023). Thermochromic Behavior of Polydiacetylene Nanomaterials Driven by Charged Peptide Amphiphiles. Biomacromolecules, 24(9), 4051-4063.

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Curli Fibril Ultrastructure Analysis

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Negative staining transmission electron microscopy (TEM) was performed on curli samples (1 mg/mL) suspended in 10 mM Tris buffer (pH 7.4) with and without incubation with 12.5 μM FSB (15 min, room temperature). Samples were applied to 300-mesh copper grids coated with Formvar film (Electron Microscopy Sciences, Hatfield, PA) for 2 min and then rinsed in deionized water. The samples were negatively stained with 2% uranyl acetate for 90 s, excess stain was wicked off with filter paper, and then the sample was air-dried. Microscopy was performed on the JEM-1400 (JEOL, LLC).

Reichhardt C, & Cegelski L. (2018). The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli. PLoS ONE, 13(8), e0203226.

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Negative Staining TEM of Curli Fibers

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Negative staining TEM was performed on curli samples. Samples were applied to 300-mesh copper grids coated with Formvar film (Electron Microscopy Sciences, Hatfield, PA) for 2 min, rinsed in deionized water, negatively stained with 2% uranyl acetate for 90 s, and air-dried. Microscopy was performed on the JEM-1400 (JEOL, LLC).

Reichhardt C., Jacobson A.N., Maher M.C., Uang J., McCrate O.A., Eckart M, & Cegelski L. (2015). Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers. PLoS ONE, 10(10), e0140388.

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Visualizing Disassembled PHF6 Fibrils

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Disassembled PHF6 peptide fibrils were prepared as mentioned in fibril disassembly assay. Samples (10 µL) were placed for 2 min on 400-mesh copper grids covered with carbon-stabilized Formvar film (Electron Microscopy Sciences (EMS), Hatfield, PA). Excess fluid was removed, and the grids were negatively stained with 2% uranyl acetate solution (10 µL) for 2 min. Finally, excess fluid was removed, and the samples were viewed using a JEM-1400 TEM (JEOL), operated at 80 kV.

KrishnaKumar V.G., Paul A., Gazit E, & Segal D. (2018). Mechanistic insights into remodeled Tau-derived PHF6 peptide fibrils by Naphthoquinone-Tryptophan hybrids. Scientific Reports, 8, 71.

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