MiR-141 mimics, mimics control, anti-miR-control and anti-miR-141 molecules were obtained from RIBOBIO (Guangzhou, China) and transfected with Lipofectamine 2000 (Invitrogen) in AGS, HGC-27 or SGC-7901 cells at a final concentration of 50 nM. siRNAs (specifically for TAZ) and control siRNA were synthesized by RIBOBIO and transfected into HGC-27 or SGC-7901 cells (100 nM) using Lipofectamine 2000 (Invitrogen). Transfection of empty vectors pcDNA3.1 and pcDNA3.1-TAZ vectors was via Lipofectamine 2000 (Invitrogen). Transfection of agomir-NC and agomir-141 was via micrON miR-141 agomir kit (RIBOBIO) according to the manufacturer's protocol.
Anti mir 141
Manufactured by RiboBio
Sourced in China
Anti-miR-141 is a synthetic oligonucleotide designed to inhibit the function of miR-141, a microRNA molecule. This product is intended for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mir control, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Anti mir nc, by RiboBio (1 mentions)
Si crnde, by RiboBio (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Si nc, by RiboBio (1 mentions)
Mir nc, by RiboBio (1 mentions)
2 protocols using anti mir 141
1
miRNA-141 Modulation in Gastric Cancer
2
Regulating CRNDE in LPS-induced Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The LPS-induced MRC-5 cells were seeded into 6-well plates and incubated in DMEM overnight. Si-CRNDE, pc-CRNDE (CRNDE), miR-141, anti-miR-141 and their negative controls (si-NC, pcDNA, miR-NC, anti-miR-NC) were purchased from Ribobio (Guangzhou, China). All plasmid and oligos were transfected into LPS-induced MRC-5 cells using Lipofectamine 2000 Reagent (Life Technologies Corporation, Grand Island, NY, USA).
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