PCB 136, 2,2′,3,3′,6,6′-hexachlorobiphenyl-4-ol (4-136), 2,2′,3,3′,6,6′-hexachlorobiphenyl-5-ol (5-136), 4,5-dimethoxy-2,2′,3,3′,6,6′-hexachlorobiphenyl (methylated derivative of 2,2′,3,3′,6,6′-hexachlorobiphenyl-4,5-diol; 4,5-136) and 2,2′,3′,4,6,6′-hexachloro-3-methoxybiphenyl (methylated derivative of 2,2′,3′,4,6,6′-hexachlorobiphenyl-3-ol; 3-150) were synthesized as described previously (Waller et al. 1999 (link)). The chemical structures and the abbreviations of the HO-PCB metabolites are shown in Figure 1A . Surrogate recovery standards (2,3,4′,5,6-pentachlorobiphenyl, PCB 117; 2′,3,3′,4,5,5′-hexachlorobiphenyl-4-ol, 4-159) and internal standard (2,2′,3,4,4′,5,6,6′-octachlorobiphenyl, PCB 204) were purchased from Accustandard (New Haven, CT). NAC and tetrabutylammonium sulfite were purchased from Fisher Scientific (Pittsburg, PA, USA). L-Glutathione and β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Pesticide grade n-hexane, 2-propanol and methyl tert-butyl ether (MTBE) were purchased from Fisher Scientific (Pittsburg, PA, USA).
Pcb 204
Manufactured by AccuStandard
Sourced in United States
The PCB 204 is a laboratory equipment designed for the analysis and identification of organic compounds. It is a high-performance liquid chromatography (HPLC) system that utilizes a photodiode array detector to provide detailed spectral data on sample components.
Automatically generated - may contain errors
Lab products found in correlation
Tetrabutylammonium sulfite, by Thermo Fisher Scientific (2 mentions)
Pcb 117, by AccuStandard (2 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
β nicotinamide adenine dinucleotide 2 phosphate reduced tetrasodium salt hydrate nadph, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Thermo Fisher Scientific (1 mentions)
Pesticide grade n hexane, by Thermo Fisher Scientific (1 mentions)
L glutathione, by Merck Group (1 mentions)
Sodium sulfite, by Thermo Fisher Scientific (1 mentions)
Reduced nicotinamide adenine dinucleotide phosphate nadph, by Merck Group (1 mentions)
Nucleodex β pm columns, by Macherey-Nagel (1 mentions)
Pcb 166, by AccuStandard (1 mentions)
D5 pcb 30, by Cambridge Isotopes (1 mentions)
3 protocols using pcb 204
1
Synthesis and Characterization of Halogenated Biphenyl Metabolites
2
Synthesis and Separation of PCB 136 Atropisomers
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Dimethyl sulfoxide (DMSO), sodium phosphate
dibasic (Na2HPO4), sodium phosphate monobasic
(NaH2PO4), magnesium chloride (MgCl2), tetrabutylammonium sulfite, sodium sulfite, and pesticide grade
solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA).
Nicotinamide adenine dinucleotide phosphate reduced (NADPH) was purchased
from Sigma-Aldrich Co. (St. Louis, MO, USA). Racemic PCB 136 was synthesized
by the Ullmann coupling of 2,3,6-trichloro-1-iodobenzene,28 and the atropisomers of PCB 136 were separated
using two serially connected Nucleodex β-PM columns (Macherey-Nagel,
Düren, Germany). The enantiomeric fractions (EF = Area(+)-PCB 136 /(Area(+)-PCB 136 + Area(−)-PCB 136)) of (−)-PCB
136 and (+)-PCB 136 were 0.01 and 1.00, respectively.27 (link) 2,2′,3,3′,6,6′-Hexachlorobiphenyl-4-ol
(4–136), 2,2′,3,3′,6,6′-hexachlorobiphenyl-5-ol
(5–136), 4,5-dimethoxy-2,2′,3,3′,6,6′-hexachlorobiphenyl,
and 2,2′,3′,4,6,6′-hexachloro-3-methoxybiphenyl
were prepared as described elsewhere.29 (link) Recovery standards (2,3,4,4′,5,6-hexachlorobiphenyl, PCB
166; 2,3,4′,5,6-pentachlorobiphenyl, PCB 117; 2,3,3′,4,5,5′-hexachlorobiphenyl-4-ol,
4′-159) and the internal standard (2,2′,3,4,4′,5,6,6′-octachlorobiphenyl,
PCB 204) were purchased from Accustandard (New Haven, CT).
dibasic (Na2HPO4), sodium phosphate monobasic
(NaH2PO4), magnesium chloride (MgCl2), tetrabutylammonium sulfite, sodium sulfite, and pesticide grade
solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA).
Nicotinamide adenine dinucleotide phosphate reduced (NADPH) was purchased
from Sigma-Aldrich Co. (St. Louis, MO, USA). Racemic PCB 136 was synthesized
by the Ullmann coupling of 2,3,6-trichloro-1-iodobenzene,28 and the atropisomers of PCB 136 were separated
using two serially connected Nucleodex β-PM columns (Macherey-Nagel,
Düren, Germany). The enantiomeric fractions (EF = Area(+)-PCB 136 /(Area(+)-PCB 136 + Area(−)-PCB 136)) of (−)-PCB
136 and (+)-PCB 136 were 0.01 and 1.00, respectively.27 (link) 2,2′,3,3′,6,6′-Hexachlorobiphenyl-4-ol
(4–136), 2,2′,3,3′,6,6′-hexachlorobiphenyl-5-ol
(5–136), 4,5-dimethoxy-2,2′,3,3′,6,6′-hexachlorobiphenyl,
and 2,2′,3′,4,6,6′-hexachloro-3-methoxybiphenyl
were prepared as described elsewhere.29 (link) Recovery standards (2,3,4,4′,5,6-hexachlorobiphenyl, PCB
166; 2,3,4′,5,6-pentachlorobiphenyl, PCB 117; 2,3,3′,4,5,5′-hexachlorobiphenyl-4-ol,
4′-159) and the internal standard (2,2′,3,4,4′,5,6,6′-octachlorobiphenyl,
PCB 204) were purchased from Accustandard (New Haven, CT).
3
Comprehensive PCB Standard Solution Composition
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Details of
PCB standard solution
can be found inTable S1 . Briefly, the
PCB standard solution was composed of (i) 209 PCB congeners (AccuStandard,
New Haven, CT, USA); (ii) 10 13C-PCB surrogate standards
(mono- to deca-chlorinated; Wellington Laboratories, Guelph, ON, Canada);
and (iii) d5-PCB30 (Cambridge Isotope Laboratories, Andover,
MA, USA), which is used as an internal standard together with PCB204
(AccuStandard). PCB congener names are in accordance with the United
States Environmental Protection Agency (US EPA).44
PCB standard solution
can be found in
PCB standard solution was composed of (i) 209 PCB congeners (AccuStandard,
New Haven, CT, USA); (ii) 10 13C-PCB surrogate standards
(mono- to deca-chlorinated; Wellington Laboratories, Guelph, ON, Canada);
and (iii) d5-PCB30 (Cambridge Isotope Laboratories, Andover,
MA, USA), which is used as an internal standard together with PCB204
(AccuStandard). PCB congener names are in accordance with the United
States Environmental Protection Agency (US EPA).44
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