STZ, SA, GPx, sodium azide, and thiobarbituric acid were obtained from the same vendor (Sigma Chemicals Co, St. Louis, MO, USA). Antibodies against Nrf2, NF-kB (p65), HO-1, HRP-conjugated secondary antibodies, and β-actin were obtained from Santa Cruz Biotechnology vendor (Dallas, TX, USA). The total protein and NE-PER cytoplasmic and nuclear extraction kit was acquired from Thermo Fisher Scientific (Waltham, MA, USA). Rat TNF-α and IL-6 ELISA kits were procured from R&D Systems, Inc. (Minneapolis, MN, USA).
Rat tnf α and il 6 elisa kits
Manufactured by R&D Systems
Sourced in United States
Rat TNF-α and IL-6 ELISA kits are laboratory assays designed to quantitatively measure the levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in rat biological samples. These kits utilize the Enzyme-Linked Immunosorbent Assay (ELISA) technique to detect and quantify the target analytes.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions)
Antibodies against nrf2, by Santa Cruz Biotechnology (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Nf κb p65 transcription factor assay kit, by Cayman Chemical (1 mentions)
Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
3 protocols using rat tnf α and il 6 elisa kits
1
Antioxidant Regulation in Oxidative Stress
2
Investigating Inflammatory Mechanisms in GM Sulfate-Induced Toxicity
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GM sulfate was obtained from Abbott Healthcare PVT Ltd, India. 2-thiobarbituric acid (TBA), antibodies against NF-κB (p65), cleaved caspase-3, Bax, Bcl-2, β-actin, and HRP-conjugated secondary antibody were purchased from Santa Cruz biotech (USA). NE-PER nuclear and cytoplasmic extraction kit was obtained from Pierce Biotechnology, Rockford, IL, (USA). NF-κB (p65) transcription factor assay kit was obtained from Cayman Chemical Company, Ann Arbor, MI. Rat TNF-α and IL-6 ELISA kits were obtained from R&D Systems, Inc. (USA). All other chemicals were of analytical grade.
3
Determining Serum Cytokine Levels
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Serum samples obtained from the heart were rapidly centrifuged at 3000 rpm for 20 min, and the serum concentrations of TNF-α and IL-6 were measured with rat TNF-α and IL-6 ELISA kits (R&D systems, Minneapolis, USA) according to the manufacturer’ instructions. The levels of TNF-α and IL-6 were expressed as pg/ml protein.
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