Von willebrand factor antibody

Manufactured by Abcam

Sourced in United States

The Von Willebrand factor antibody is a laboratory reagent used to detect and measure the presence of von Willebrand factor, a blood glycoprotein involved in hemostasis.

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Lab products found in correlation

Sirt1 antibody, by Santa Cruz Biotechnology (2 mentions) Bx61 microscope, by Olympus (2 mentions) Caveolin 1, by Abcam (1 mentions) 8 ohdg, by Abcam (1 mentions) Tissue tek oct embedding medium, by Sakura Finetek (1 mentions) Alexa fluor 488 or alexa fluor 568 labeled goat anti rabbit antibodies, by Thermo Fisher Scientific (1 mentions) Axioskop 2 microscope, by Zeiss (1 mentions) Axiocam, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Alexa fluor 555 labeled secondary antibody, by Beyotime (1 mentions)

5 protocols using von willebrand factor antibody

Immunofluorescence Analysis of Vascular Proteins

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Aortic and mesenteric arteries sections were de-paraffinized with xylene, followed by antigen retrieval by heating in citrate buffer (10 mM). Sections were probed with appropriate primary antibodies. Sirt1 antibody (Santa Cruz Biotech, Dallas, TX), Caveolin-1, Binding immunoglobulin protein (BiP), von Willebrand factor antibody (Abcam, Cambridge, MA) were used at a 1:50 to 1:200 dilution followed by a biotinylated secondary antibody for immunofluorescence. Sections were digitally imaged with an Olympus BX-61 microscope.

Kassan M., Vikram A., Kim Y.R., Li Q., Kassan A., Patel H.H., Kumar S., Gabani M., Liu J., Jacobs J.S, & Irani K. (2017). Sirtuin1 protects endothelial Caveolin-1 expression and preserves endothelial function via suppressing miR-204 and endoplasmic reticulum stress. Scientific Reports, 7, 42265.

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Immunofluorescent Analysis of Oxidative Stress

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MRA segments were frozen in Tissue Tek OCT embedding medium (Sakura Finetek Europe, The Netherlands). Transverse sections were cut 5-μm thick. After blockade in PBS containing 5% fetal bovine serum and 0.3% Triton X-100, sections were incubated with primary antibodies against 8-hydroxydeoxyguanosine (8-OHDG) (Abcam, Cambridge, MA), and von Willebrand factor antibody (Abcam, Cambridge, MA) used at a 1:50–1:200 dilution followed by a biotinylated secondary antibody for immunofluorescence. Immunofluorescent signals were viewed using an Eclipse 55i fluorescence microscope (x20), Nikon.

Chen C., Kassan A., Castañeda D., Gabani M., Choi S.K, & Kassan M. (2019). Metformin prevents vascular damage in hypertension through the AMPK/ER stress pathway. Hypertension research : official journal of the Japanese Society of Hypertension, 42(7), 960-969.

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Immunohistochemical Analysis of Aortic Tissues

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Paraffin blocks of aortas and MRAs were de-paraffinized with xylene followed by antigen retrieval by heating in citrate buffer (10 mM). Sections were probed with appropriate primary antibodies. Sirt1 antibody (Santa Cruz Biotech, Dallas, TX), Binding immunoglobulin protein (BIP), and C/EBP homologous protein (CHOP) antibodies (Cell Signaling, Denver, MA, USA), von Willebrand factor antibody and 8-hydroxydeoxyguanosine (8-OHdG) antibodies (Abcam, Cambridge, MA,) were used at a 1:50 to 1:200 dilution followed by a biotinylated secondary antibody for immunofluorescence, or streptavidin peroxidase solution, DAB peroxidase substrate, and hematoxylin counterstain for immunohistochemistry. Sections were digitally imaged with an Olympus BX-61 microscope. Images of vessels and cells were taken at 40 × and 60 × magnification, respectively.

Kassan M., Vikram A., Li Q., Kim Y.R., Kumar S., Gabani M., Liu J., Jacobs J.S, & Irani K. (2017). MicroRNA-204 promotes vascular endoplasmic reticulum stress and endothelial dysfunction by targeting Sirtuin1. Scientific Reports, 7, 9308.

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Immunofluorescence Analysis of Vascular Markers

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Rabbit polyclonal von Willebrand factor antibody (Abcam, Cambridge, England), anti-CD31 (PECAM) antibody (Abcam), anti-platelet-derived growth factor (PDGF)-beta antibody (Abcam), and anti-alpha smooth muscle actin (aSMA) antibody (Abcam) were purchased, and Alexa Fluor 488-or Alexa Fluor 568-labeled goat anti-rabbit antibodies from Invitrogen were used. The 5-μm sections were fixed in 4% PFA, then incubated with primary antibodies for immunohistochemistry and appropriate secondary antibodies. An Axioskop 2 microscope (Carl Zeiss Microimaging, Welwyn Garden City, UK) equipped with an AxioCam (Carl Zeiss Microimaging) was used to observe tissues and take photographs. Fluorescence intensity was measured using Photoshop version 7.0 software (Adobe Systems Incorporated, San Jose, CA). The intensities of control samples were designated as 1. The results per view are expressed as mean ± standard deviation (SD).

Kitano H., Mamiya A., Ishikawa T., Fujiwara Y., Masaoka Y., Miki T, & Hidai C. (2020). An Epidermal Growth Factor Motif of Developmental Endothelial Locus 1 Protein Inhibits Efficient Angiogenesis in Explanted Squamous Cell Carcinoma In Vivo. Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 73(1).

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Immunofluorescent Staining of Lung Tissue

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Five-micron sections from 10% formalin paraffin-fixed lung tissues were blocked and incubated with von willebrand factor antibody (Abcam, USA) followed by Alexa Fluor 555-labeled secondary antibody (Beyotime Inc., China). PMVECs were fixed with 10% formalin for 30 min, permeability with 0.3% Triton X-100 for 10 min and blocked with 10% goat serum for 1 h at room temperature. Cells were then incubated with phalloidin-rhodamine for 20 min for staining off-actin. DAPI was used for nuclear staining (Beyotime Inc., China). Micrographs were obtained with a fluorescent microscope (Olympus BX51, Japan).

Chen L., Han Y., Li Y., Chen B., Bai X., Belguise K., Wang X., Chen Y., Yi B, & Lu K. (2019). Hepatocyte-derived exosomal MiR-194 activates PMVECs and promotes angiogenesis in hepatopulmonary syndrome. Cell Death & Disease, 10(11), 853.

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