Agilent 1260 dad g4212b

In the present work, 14 PAHs were analyzed (see Table S1 for their structural formulas), namely naphthalene (Naph), acenaphthylene (Acy), phenanthrene (Phen), anthracene (Ant), fluoranthene (Flt), pyrene (Pyr), benzo[a]anthracene (BaA), chrysene (Chry), benzo[b]fluoranthene (BbF), benzo[k])fluoranthene (BkF), benzo[a]pyrene (BaP), dibenzo[a,h]anthracene (DahA), benzo[g,h,i]perylene (BghiP) and indeno[1,2,3-cd]pyrene (IcdP). The quantitative analysis of these PAHs was carried out by a HPLC (Agilent 1260 system) equipped with a ZORBAX Eclipse PAH column (4.6 × 50 nm, 3.5 µm) and an UV detector (Agilent 1260 DAD G4212B) set to wavelengths (λ) of 220 nm, 230 nm and 254 nm. Analytes were separated by gradient elution with acetonitrile (A) and water (B) as mobile phase, at a flow rate of 1.4 mL/min, the injection volume set to 20 μL and the column temperature at 20 °C. The elution program was defined as follows: 0–6 min isocratic 40:60 (v/v) A:B; 6–9.5 min linear gradient from 40 to 100% of A and 9.5–12 min isocratic 40:60 (v/v) A:B. Peak identification and integration were performed by external standard method with the ChemStation software (Agilent Technologies).

The samples were analyzed by a HPLC (Agilent 1260 system) using a ZORBAX Eclipse PAH column (4.6 × 50 nm, 3.5 µm) and a UV detector (Agilent 1260 DAD G4212B) operating with wavelengths (λ) of 220 nm, 230 nm, and 254 nm. The column temperature was maintained at 25 °C, the injection volume was set to 20 μL, and the flow rate was 1.4 mL/min. The elution program was defined as follows (with acetonitrile (A) and water (B) as mobile phases): 0–6 min isocratic 40:60 (v/v) A:B; 6–9.5 min linear gradient from 40 to 100% of A and 9.5–12 min isocratic 40:60 (v/v) A:B. The peak intensity of each PAH changes depending on the UV wavelength; thus, the PAHs were calibrated at the wavelength where the intensity was greatest for that PAH. Table 1 shows the retention time and the UV wavelength at which the peak of each PAH was most intense. The PAH peaks in the sample chromatograms were identified by a retention time matching between standard and sample chromatograms. Quantification was performed by the peak area of each PAH using the ChemStation software (Agilent Technologies, Santa Clara, CA, USA).