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2 protocols using pooled human plasma

1

Quantification of Opioid Analogs in Plasma

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Naltrexone (NTX), 6‐beta‐naltrexol (6βN), deuterium‐labeled naltrexone (NTX‐D3), and deuterium labeled 6‐beta‐naltrexol (6βN‐D3) certified reference materials were purchased from Sigma Aldrich (St. Louis, MO). Ammonium hydroxide, optima grade methanol, and optima grade formic acid were also purchased from Sigma Aldrich. Pooled human plasma (N = 50, mixed sex) with ethylenediaminetetraacetic acid (EDTA) as anticoagulant was obtained from BioIVT (Westbury, NY).
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2

Lipid Standards for Metabolomic Analysis

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1,2-dipentadecanoyl-sn-glycero-3-phosphoethanolamine (PE 15:0/15:0), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE 16:0/18:1(9Z)), 1-(1Z-octadecenyl)-2-arachidonoyl-sn-glycero-3-phosphoethanolamine (PE P-18:0/20:4(5Z,8Z,11Z,14Z)), 1-tridecanoyl-sn-glycero-3-phosphoethanolamine (LPE 13:0/0:0) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). All of them were >99% pure, except LPE 13:0/0:0 may contain up to 10% of sn-2 isomer. [U-13C6]-D-glucose (99%) was from Cambridge Isotope Laboratories (Tewksbury, MA, USA). D-glucose and all solvents including methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), chloroform (CHCl3), and water (H2O) of LC-MS grade and HPLC grade were purchased from Fisher Scientific (Pittsburgh, PA, USA). Pooled human plasma collected from healthy people with K2-EDTA as anticoagulant was purchased from BioIVT (Westbury, NY, Lot#: BRH1524451). The standard reference material—metabolites in frozen human plasma (NIST SRM-1950)—was purchased from the NIST (Gaithersburg, MD).
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