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4 protocols using succinic acid

1

Manganese-Catalyzed Oxidative Silica Nanoparticles

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Cetyltrimethylammonium chloride (CTAC, 25 wt%), tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTES), MnSO4·H2O, glucose oxidase, and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were obtained from Sigma-Aldrich. Triethanolamine (TEA) and methylene blue (MB) were obtained from Aladdin Industrial Inc. Disodium maleate, succinic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), and 1-hydroxybenzotriazole monohydrate (HOBT) were obtained from Tokyo Chemical Industry Co., Ltd. Polyethylene imine (PEI, molecular weight 5000, 50 wt%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Small interfering RNA against Twist (anti-Twist siRNA) (5′-GCU GAG CAA GAU UCA GAC CTT-3′ (sense)), FAM-labeled scrambled RNA (FAM siRNA), and Cy5-labeled scrambled RNA were obtained from Guangzhou Ribo Bio-Technology Co., Ltd. RNase A and RNase inhibitor were bought from the Beyotime Institute of Biotechnology. The anti-Twist, anti-beta actin, and anti-MMP-9 were purchased from Abcam Biotechnology. The anti-uPA antibody was obtained from Santa Cruz Biotechnology.
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Synthesis of Aspartame Precursor

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l-Aspartyl-l-phenylalanine methyl ester was supplied by Ajinomoto Co., Inc. Triphenyl phosphite (P(OPh)3), l-phenylalanine (Phe), succinic acid, ethylene diamine and p-phenylenediamine were purchased from Tokyo Chemical Industry Co., Ltd. 5% Palladium on activated carbon (Pd/C) and N-methylpyrrolidone (NMP) were purchased from FUJIFILM Wako Pure Chemical Corporation. Dimethyl sulfoxide (DMSO), sulfuric acid, nitric acid, pyridine, ethanol, tetrahydrofuran, ethyl acetate, acetone and toluene were purchased from Kanto Chemical Co., Inc. All the chemicals were directly used as purchased.
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Analytical Characterization of Organic Compounds

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The chemicals, their sources, purity, and CAS numbers were adipic acid,
TCI, ≥ 99%, 124-04-9; ammonium nitrate, Fisher, ACS grade, 6484-52-2;
citric acid monohydrate, Fisher, ACS grade, 5949-29-1; DL-3-hydroxybutyric acid
sodium salt, Chem Impex Int’l Inc., 100.30%, 150-83-4 & 306-31-0;
DL-malic acid, Alfa Aesar, 98%, 6915-15-7; glutaric acid, Acros organics,
99%,110-94-1; hydrogen peroxide 3% W/W, BDH chemicals, 7722-84-1; horseradish
peroxide type II, Sigma, 150-250 U/mg, 9003-99-0; lactic acid, TCI, ≥
85%, 50-21-5; pimelic acid, Alfa Aesar, 98+%, 111-16-0; sodium acetate, VWR, ACS
grade, 127-09-3; sodium azide, Sigma, 99.8%, 26628-22-8; sodium nitrate, BDH
chemicals, ACS grade, 7631-99-4; succinic acid, TCI America, ≥
99%,110-15-6; and tricarballylic acid, Alfa Aesar, 98%, 99-14-9. The electron
microscopy grids were 200 mesh carbon support film on hexagonal copper square
grids from Electron Microscopy Sciences. Pierce Biotechnology’s 2 kD MWCO
Slide-A-Lyzer™ dialysis cassettes were used. Trace metal grade
concentrated nitric acid was from Fisher. ICP/DCP Ce ion standard solution was
from Aldrich.
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4

Chromate Reduction Reaction Kinetics

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All solutions were prepared with ultrapure Millipore deionized water obtained via a Milli-Q® Advantage A10® Direct water purification system, with a resistivity of 18.2 MΩ cm at 25.0 °C. The following chemicals were used as received without further purification: potassium chromate (Alfa Aesar, 99.0%), sodium dihydrogen citrate (Alfa Aesar, 99.0%), citric acid-disodium salt sesquihydrate (Alfa Aesar, 99.0%), glacial acetic acid (Millipore, ACS grade), sodium acetate anhydrous (Millipore, ACS grade), glycolic acid (Acros, 99.0%), succinic acid (TCI America, ≥99.0%), disodium succinate anhydrous (Acros, 99.0%), dl-malic acid (TCI America, ≥99.0%), propanoic acid (TCI America, ≥99.0%), potassium propionate (TCI America, ≥98.0%), potassium nitrate (Avantor Performance Materials US, 99.0–100.5%), potassium hydroxide (BDH Chemicals, 85.0%), and hydrochloric acid (BDH Chemicals, 36.5–38%). Potassium chloride (BDH Chemicals, 99.0–100.5%) was recrystallized by slow diffusion of ethanol into a supersaturated KCl solution in water and dried under vacuum before use.
Concentrations of Cr(vi) stated throughout correspond to the value calculated based on the added K2CrO4. As reported previously,7,21 (link) dichromate is likely present at the higher 7.00 mM Cr(vi) concentrations used.
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