The source for all mass cytometry antibodies can be found in Table S5 . Antibodies were conjugated to their associated metals with MaxPar X8 labeling reagent kits (Fluidigm) according to manufacturer instructions, diluted with Candor PBS Antibody Stabilization solution (Candor Bioscience, CAT#130 050) supplemented with 0.02% sodium azide, and filtered through an UltrafreeMC 0.1-mm centrifugation filter (Millipore) before storage at 4°C. To reduce tube-to-tube pipetting variations, part of the signaling antibody panel came from lyophilized antibody cocktail, made at Stanford University as previously described ((Han et al., 2018 (link))). Surface and intracellular master antibody cocktails were made and kept at −80°C in order to stain up to 600 samples.
Ultrafreemc 0.1 mm centrifugation filter
Manufactured by Merck Group
The UltrafreeMC 0.1-mm centrifugation filter is a laboratory equipment product designed for sample preparation. It is used to separate and isolate macromolecules, such as proteins, from small molecules or other contaminants in a sample through the process of centrifugation. The filter has a pore size of 0.1 mm, which allows for efficient separation and concentration of the desired components.
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Lab products found in correlation
3 protocols using ultrafreemc 0.1 mm centrifugation filter
1
Mass Cytometry Antibody Preparation
2
Mass Cytometry Antibody Conjugation and Storage
3
Mass Cytometry Antibody Labeling and Staining
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The source for all mass cytometry antibodies can be found in Supplementary Table 1 . Antibodies were conjugated to their associated metals with MaxPar X8 labeling reagent kits (Fluidigm) according to manufacturer instructions, diluted with Candor PBS Antibody Stabilization solution (Candor Bioscience, CAT#130 050) supplemented with 0.02% sodium azide, and filtered through an UltrafreeMC 0.1-mm centrifugation filter (Millipore) before storage at 4° C. To reduce tube-to-tube pipetting variations, part of the signaling antibody panel came from lyophilized antibody cocktail, made at Stanford University as previously described ((Han et al. 2018 (link))). Surface and intracellular master antibody cocktails were made and kept at −80° C in order to stain up to 600 samples.
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