Rabbit serum

Manufactured by Abcam

Sourced in United Kingdom

Rabbit serum is a laboratory reagent derived from the blood of rabbits. It is commonly used as a source of proteins, antibodies, and other biomolecules in various research and diagnostic applications.

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Lab products found in correlation

Goat serum, by Abcam (1 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) Aec substrate chromogen, by Agilent Technologies (1 mentions) Target retrieval solution, by Agilent Technologies (1 mentions) Haematoxylin, by Merck Group (1 mentions) Facscalibur, by FlowJo (1 mentions) Canto 2, by BD (1 mentions) Mouse serum, by Abcam (1 mentions) Biotinylated goat anti rabbit igg, by Boster Bio (1 mentions) 3 3 diaminobenzidine (dab), by ZSGB-BIO (1 mentions)

3 protocols using rabbit serum

Immunohistochemical Staining Protocol

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Tissues were embedded in paraffin (ROTH) and cut in 2-μm-thick slices. Immunohistochemistry was carried out as described previously[17 (link)]. Briefly, after deparaffinization and blocking for endogenous H₂O₂, the slides were incubated in 1 x target retrieval solution (Dako) at 98 °C for 20 min. For FAH (primary antibody, Abcam, ab81087) staining, tissues were blocked with the Avidin/Biotin blocking kit (Vector laboratories). Goat serum (Abcam) or rabbit serum (Abcam) was then used for blocking. Biotinylated goat anti-rabbit and rabbit anti-goat secondary antibodies (Vectastain, Vector laboratories) were used. Colour development was conducted using AEC substrate chromogen (Dako). Counterstaining was performed using haematoxylin (Merck Millipore, Germany).

Junge N., Yuan Q., Vu T.H., Krooss S., Bednarski C., Balakrishnan A., Cathomen T., Manns M.P., Baumann U., Sharma A.D, & Ott M. (2018). Homologous recombination mediates stable Fah gene integration and phenotypic correction in tyrosinaemia mouse-model. World Journal of Hepatology, 10(2), 277-286.

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Neuronal Differentiation Flow Cytometry

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Flow cytometry analysis was performed at the NPC and the final neuronal stages. Cells were harvested by enzymatic dissociation using Accutase for 5 min at 37°C until cells were visibly detached. Cells were pelleted by centrifugation at 800 rpm for 3 minutes, resuspended in ice-cold PBS. Cells were fixed in ice-cold 1%PFA in PBS and permeabilized in 0.1% TritonX-100 in PBS. Cells were blocked in 10% Rabbit serum (Abcam) and 10% Mouse serum (Abcam) for 1h. Cells were then resuspended in blocking buffer containing the appropriate concentration of conjugated antibody according to the manufacturer’s specifications (see Supplementary Table S3 for the list of the antibodies used in this study). Cells were sorted with Becton Dickinson Canto II, and Becton Dickinson FacsCalibur, followed by analysis using FlowJo 10.2 (FlowJo, LLC)

Tagliafierro L., Glenn O.C., Zamora M.E., Beach T.G., Woltjer R.L., Lutz M.W, & Chiba-Falek O. (2017). Genetic analysis of SNCA 3’UTR and its corresponding miRNAs in relation to Parkinson’s compared to Dementia with Lewy Bodies. Alzheimer's & dementia : the journal of the Alzheimer's Association, 13(11), 1237-1250.

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Immunohistochemical Quantification of Substance P in Lung Tissue

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Lung tissue (size of 0.8 × 0.6 × 0.2 cm) was cut into continuous coronal slices (45 μm thickness per slice). Briefly, the slices were placed in deionized water containing 3 % H 2 O 2 , rinsed in PBS (5 min, three times), incubated in rabbit serum (Abcam, UK) at room temperature for 4 h, followed by incubation with goat anti-SP polyclonal antibody (1:200, Santa Cruz, USA) at 4 °C overnight, then rinsed in PBS for 5 min. The slices were sequentially incubated with biotinylated rabbit antigoat IgG (Boster, China) for 2 h, rinsed in PBS (5 min, three times), incubated with SABC (Boster, China) for 1 h, and followed by PBS rinsing (5 min, three times). The slices were stained with DAB (ZSGB-BIO, China) and dehydrated using graded ethanol and dimethylbenzene. The slices were sealed with a neutral resin under a microscope. Image-Pro Plus was used to calculate mean optical density (MOD) of SP in the lungs. The five random slices per animal were chosen for calculation and the mean data of five slices represented the value per animal.

Ji Z., Wang Z., Chen Z., Jin H., Chen C., Chai S., Lv H., Yang L., Hu Y., Dong R, & Lai K. (2018). Melatonin attenuates chronic cough mediated by oxidative stress via transient receptor potential melastatin-2 in guinea pigs exposed to particulate matter 2.5. Physiological research, 67(2).

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