BALB/c mice were anesthetized by intraperitoneal injection of 150 µl ZRF mixture containing 250 mg/ml Zoletil Forte (Virbac, Carros, France), 20 mg/ml Rompun (Bayer Animal Health), and 50 μg/ml fentanyl (Actavis, Parsippany-Troy Hills, NJ, USA). After shaving the lower back, 25 µl of DNA vaccine (0.5 µg/µl in 0.9% NaCl) was injected intradermally (i.d.) on the left and right flanks. Immediately after injection, the skin was electroporated using the Derma Vax (Cyto Pulse Sciences, Inc., Glen Burnie, MD, USA) system with two pulses of 450 V/cm × 2.5 µs and eight pulses of 110 V/cm × 8.1 ms.
Zoletil forte
Manufactured by Virbac
Sourced in France
Zoletil Forte is a veterinary anesthetic and sedative product manufactured by Virbac. It is a combination of two active ingredients, tiletamine and zolazepam, which work together to induce a state of general anesthesia and muscle relaxation in animals. The product is primarily used in veterinary settings for the immobilization and sedation of animals during various medical procedures or examinations.
Automatically generated - may contain errors
Lab products found in correlation
Rompun, by Bayer (10 mentions)
Endofree plasmid mega kit, by Qiagen (3 mentions)
Nacl solution, by B. Braun (1 mentions)
Rnalater, by Merck Group (1 mentions)
Batf3 mice, by Jackson ImmunoResearch (1 mentions)
Isoflurane, by Baxter (1 mentions)
Female balb c mice, by Janvier Labs (1 mentions)
Dermavax, by Harvard Apparatus (1 mentions)
Pandemrix, by GlaxoSmithKline (1 mentions)
Ringer acetate, by Baxter (1 mentions)
Servo i, by Getinge (1 mentions)
Midazolam, by Accord Healthcare (1 mentions)
Endofree kit, by Qiagen (1 mentions)
11 protocols using zoletil forte
1
DNA Vaccine Delivery in BALB/c Mice
2
DNA Vaccine Delivery by Electroporation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BALB/c mice were anesthetized with 150 ZRF cocktail (250 mg/ml of Zoletil Forte (Virbac, France), 20 mg/ml Rompun (Bayer Animal Health, GmbH) and 50 μg/ml of Fentanyl (Actavis, Germany)). After shaving the lower back, 25 µl of DNA vaccine (0.5 µg/µl in 0.9% NaCl) was injected i.d. on the left flank followed by electroporation using the DermaVax (Cyto Pulse Sciences, Inc) system with 2 pulses of 450 V/cm × 2.5 µs and 8 pulses of 110 V/cm × 8.1 ms. The procedure was repeated on the right flank.
3
Murine DNA Vaccine Delivery Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized (0.1 mg/10 g [body weight] with a cocktail composed of Zoletil Forte [250 mg/ml; Virbac France], Rompun [20 mg/ml; Bayer Animal Health GmbH], and fentanyl [50 μg/ml; Actavis, Germany]) by intraperitoneal (i.p.) injection. For i.d. delivery of vaccines, the lower back region of each mouse was shaved, and 12.5 μg of plasmid in a 25-μl volume was injected at two sites (total DNA/mouse, 25 μg), immediately followed by skin electroporation (DermaVax; Cellectis, Paris, France). For i.m. delivery of vaccines, mice were shaved on each leg, and 6.25 μg of DNA was injected in a 50-μl volume into each quadriceps femoris (total DNA/mouse, 12.5 μg). Immediately after injection, electrical pulses were applied at the injection site (Elgen; Inovio Biomedical Co., Blue Bell, PA). All DNA vaccines were purified by using an EndoFree Plasmid Mega kit (catalog no. 12381; Qiagen, Hilden, Germany) and dissolved in sterile injection fluid (0.9% NaCl).
4
Malaria Vaccine Delivery via DNA and Adenovirus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized by intraperitoneal (i.p.) injection with 0.1 mg/10 g body weight with a cocktail of; Zoletil Forte (250 mg/ml, Virbac), Rompun (20 mg/ml, Bayer Animal Health GMBH), and Fentanyl (50 µg/ml, Actavis). All DNA vaccines were purified by EndoFree Plasmid Mega Kit (Qiagen) and dissolved in sterile 0.9% NaCl solution (B. Braun). For intramuscular (i.m.) delivery of DNA vaccines, mice were shaved on each leg, and 25 µg of PfRH5ΔNL-containing DNA or 2.5 µg of PvDBP-containing DNA was injected in a 50 µl volume into each quadriceps femoris muscle (50 µg or 5 µg total DNA/mouse, respectively). Immediately after injection, electrical pulses were applied at the injection site (Elgen 1000 Needle Electroporator, Inovio Biomedical Co., Blue Bell, PA, USA) as described (24 (link)). 1x1010 infectious units (IU)/mouse were injected i.m. with AdHu5 containing PfRH5FL (33 (link)) in the adenoviral boost.
5
Murine DSS-Induced Colitis Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were collected on day 6 of DSS exposure. Initially, whole blood was collected by cardiac puncture following anesthesia by a cocktail of Zoletil Forte (Virbac), Rompun (Bayer), and Fentadon (Eurovet Animal Health) (ZRF; intraperitoneally 0.1 mL ZRF/10 g body weight), with the following active ingredients: zolezepam (32 mg/kg), tiletamine (32 mg/kg), xylazine (4.5 mg/kg), and fentanyl (26 μg/kg). Blood (0.5–1 mL) was drawn into tubes containing ~50 µL NaEDTA (50 mM) as anticoagulant and mice were then killed by cervical dislocation. Blood was centrifuged (6000 × g, 10 min, 4°C) to obtain plasma. Colon mucosa was collected by opening the colon longitudinally and kept in RNAlater (Sigma-Aldrich). Fecal pellets were collected from the colon. All samples were stored at –80°C. Due to failure of collecting and processing some of the samples, the number of data points differed occasionally between groups.
6
MRSA Wound Infection Model in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anaesthetised with a ZRF cocktail before wound preparation and the inoculation of MRSA. The ZRF cocktail consists of 3.3 mg Zoletil Forte (Virbac, Carros, France), 0.5 mg Rompun (Bayer, Oslo, Norway), and 2.6 μg Fentadon (Eurovet Animal Health, Bladel, The Netherlands) per ml 0.9% NaCl. ZRF was administered by intraperitoneal injection at 0.1 mL ZRF/10 g body weight.
After anaesthetising the mice, fur on the back and flanks were removed first with an electrical hair trimmer (Wella Professional Contura HS 61 Trimmer). The remaining fur was removed with a hair removal cream (Veet, Reckitt Benckiser, Slough, United Kingdom) to secure Tegaderm adhesion. One wound was made in the skin on the back of each mouse with a 6 mm sterile disposable biopsy punch (Miltex Instruments, Bethpage, NY, USA). Each wound was inoculated with 20 μL of ice‐cold phosphate‐buffered saline (PBS) containing 6 × 107 CFUs of S. aureus Xen31 using a pipette with tip. After bacterial application, the mice were kept on a warm pad for 10–15 min to dry the inoculum. Wounds of all the mice from all groups were then covered with a 4 × 5 cm Tegaderm film to prevent contractures of the skin and to keep the wound from later drying out and crusting. The Tegaderm also keeps the mice form licking and cleaning the wound. The mice were then placed back in cages for 24 hours to establish the wound infection.
After anaesthetising the mice, fur on the back and flanks were removed first with an electrical hair trimmer (Wella Professional Contura HS 61 Trimmer). The remaining fur was removed with a hair removal cream (Veet, Reckitt Benckiser, Slough, United Kingdom) to secure Tegaderm adhesion. One wound was made in the skin on the back of each mouse with a 6 mm sterile disposable biopsy punch (Miltex Instruments, Bethpage, NY, USA). Each wound was inoculated with 20 μL of ice‐cold phosphate‐buffered saline (PBS) containing 6 × 107 CFUs of S. aureus Xen31 using a pipette with tip. After bacterial application, the mice were kept on a warm pad for 10–15 min to dry the inoculum. Wounds of all the mice from all groups were then covered with a 4 × 5 cm Tegaderm film to prevent contractures of the skin and to keep the wound from later drying out and crusting. The Tegaderm also keeps the mice form licking and cleaning the wound. The mice were then placed back in cages for 24 hours to establish the wound infection.
7
Anesthesia and Blood Sampling in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female BALB/c mice aged 6–8 weeks old were purchased from Janvier, France. BATF3-/- mice on a BALB/c background were purchased from The Jackson Laboratory (Stock No.: 013755) and bred in-house. Mice were anesthetized with i.p. injection of a cocktail of Zoletil forte (250 mg/mL, Virbac, Carros, France), Rompun (20 mg/mL, Bayer Animal Health) and Fentanyl (50 ug/mL, Actavis, Parsippany-Troy Hills, New Jersey, USA) (ZRF) given at 6 mL/kg bodyweight, or isoflurane (Baxter Healthcare, Deerfield, Illinois, USA). Blood samples were collected from vena saphena or by cardiac puncture (at endpoint). Mice were euthanized by cervical dislocation.
8
Intradermal DNA Vaccine Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mice were anesthetized with 150 μl (no more than 0.01 mg/g bodyweight) of ZRF mixture consisting of 250 mg/ml Zoletil Forte (Virbac), 20 mg/ml Rompun (Bayer Animal Health), and 50 μg/ml Fentanyl (Actavis). The lower dorsal region was shaved and injected intradermally (i.d.) with 25 μl of saline solution containing the DNA vaccine (0.5 mg/ml DNA in NaCl) on one side of the lower flank, the injection site was immediately electroporated (Two pulses of 450 V/cm x 2.5 μs and eight pulses of 110 V/cm × 8.1 ms) using a needle array electrode and a DermaVax (BTX Harvard Apparatus, Holliston, MA). The procedure was repeated for the other flank, and each mouse received a total of 25 μg DNA.
9
Influenza DNA Vaccine with Electroporation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anesthetized mice [0.1mg/10g: cocktail of Zoletil Forte (250mg/ml; Virbac France), Rompun (20mg/ml; Bayer Animal Health GmbH), and fentanyl (50µg/ml; Actavis, Germany)] were vaccinated intra muscularly (i.m.) with 25µg DNA (αMHCII-HA) into each quadriceps femoris, immediately followed by electroporation over the injection site (Elgen; Inovio Biomedical Co., Blue Bell, PA). The αMHCII-HA plasmid encodes HA from influenza A/California/07/2009 (H1N1), aa 18-541, linked to the MHCII-specific scFv via a dimerization unit consisting of the CH3 domain of human IgG3 (22 (link)). All DNA vaccines were purified by using an EndoFree Plasmid Mega kit (catalog no. 12381; Qiagen, Hilden, Germany) and dissolved in sterile injection fluid (0.9% NaCl). Alternatively, anaesthetized mice were vaccinated i.m. with 1/10 human dose of Pandemrix with AS03 adjuvant (GlaxoSmithKline, Belgium), or a non-adjuvanted trivalent inactivated seasonal influenza vaccine [strains: A/Michigan/45/2015 (H1N1)pdm09-like virus, A/Singapore/INFIMH-16-0019/2016(H3N2)-like virus B/Colorado/06/2017-like virus (B/Victoria/2/87 lineage)].
10
Anesthesia and Ventilation Protocol in Pigs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pigs were premedicated with Zoletil Forte (tiletamine/zolazepam) at 6 mg/kg (Virbac, Kolding, Denmark) and Rompun (xylazine) at 2.2 mg/kg (Elanco Denmark Aps, Ballerup, Denmark) and given a bolus of fentanyl at 5 μg/kg (Braun, Danderyd, Sweden) when intravenous access was established. Anesthesia was maintained with ketamine (Abcur, Helsingborg, Sweden) at 30 mg/kg/h, fentanyl (Braun, Danderyd, Sweden) at 4 μg/kg/h, and midazolam (Accord Healthcare, Solna, Sweden) at 0.12 mg/kg/h during the whole experiment. After adequate levels of anesthesia and analgesia were ascertained by the absence of reaction to pain stimulus between the rear hooves, rocuronium (Braun, Kista, Sweden) at 2.5 mg/kg/h was infused intravenously as a muscle relaxant. Ringer acetate (Baxter, Kista, Sweden) was infused intravenously at a rate of 10 mL/kg/h during the first hour and thereafter at a rate of 5 mL/kg/h. Animals were tracheostomized and mechanically ventilated (Servo I, Maquet, Solna, Sweden).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!