L lysine hcl

Manufactured by Merck Group

Sourced in Germany

L-lysine HCl is a versatile laboratory reagent used in various applications. It is the hydrochloride salt of the essential amino acid L-lysine. L-lysine HCl serves as a building block for proteins and is widely used in biochemical research, cell culture media preparation, and analytical techniques.

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Lab products found in correlation

L arginine hcl, by Merck Group (2 mentions) L proline, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Epinephrine, by PromoCell (1 mentions) Transferrin, by PromoCell (1 mentions) Cacl2, by PromoCell (1 mentions) Nahco3, by PAN Biotech (1 mentions) Mouse gm csf, by R&D Systems (1 mentions) Silac rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Rpmi 1640, by Corning (1 mentions) Dmem d5030, by Merck Group (1 mentions) Pnipam, by Merck Group (1 mentions) 2 hydroxyethyl methacrylate hema, by Merck Group (1 mentions) L isoleucine, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) L leucine, by Merck Group (1 mentions)

Close spelling variants of this product

[13C6,15N2]L-lysine-HCl Nε-Boc-l-lysine methyl ester hydrochloride (H-Lys(Boc)-OMe·HCl), Lysine HCl L-lysine

5 protocols using l lysine hcl

SILAC Labeling for Proteomics Analysis

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SILAC labelling was performed as previously described [15] (link), [16] (link), briefly: NHF were cultured and passaged in SILAC-DMEM with 4.5 g/L glucose, sodium pyruvate and 3.7 g/L NaHCO₃ (PAN Biotech), supplemented with 10% dialyzed FBS (Gibco), 1% penicillin/streptomycin, 82 mg/L l-proline, 84 mg/L l-arginine HCl (Arg0) and 146 mg/L l-lysine HCl (Lys0) for light labelling (Sigma-Aldrich). For medium-heavy labelled cells, Arg0 and Lys0 were replaced by l-arginine-¹³C₆¹⁴N₄ and l-lysine-²H₄ (Arg6, Lys4). For heavy labelling, l-arginine-¹³C₆¹⁵N₄ and l-lysine-¹³C₆¹⁵N₂ (Arg10, Lys8) were used.
SCC13 cells were cultured in SILAC-Keratinocyte Growth Medium 2 (KGM2) without calcium, l-arginine, and l-lysine (PromoCell), supplemented with SupplementMix containing 0.004 mL/mL bovine pituitary extract, 0.125 ng/mL epidermal growth factor, 5 μg/mL insulin, 0.33 μg/mL hydrocortisone, 0.39 μg/mL epinephrine, 10 μg/mL transferrin and 0.06 μM CaCl₂ (PromoCell), with 1% penicillin/streptomycin. l-Lysine HCl concentrations were the same as mentioned above. For l-arginine HCl, 210 mg/L were used [17] (link).

Vu B., Souza G.R, & Dengjel J. (2021). Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome. Matrix Biology Plus, 11, 100066.

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Bone Marrow Dendritic Cell Culture

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BMDC-RPMI: 10% fetal bovine serum (FBS, HyClone), 1% L-glutamine (Gibco), 1% Penicillin/Streptomycin (P/S, Gibco), 55μM 2-ME (Sigma), and 10μg/mL mouse GM-CSF (R&D Systems) in RPMI-1640 (Corning). Complete (C)-DMEM: 10% bovine calf serum (BCS, HyClone,) and 1% P/S in standard DMEM (Corning). L-ARG-free SILAC RPMI: 10% dialyzed FBS (Corning), 1% P/S, 219μM L-lysine-HCl (Sigma), and 55μM 2-ME in SILAC RPMI-1640 (Thermo Scientific).

Crowther R.R., Schmidt S.M., Lange S.M., McKell M.C., Robillard M.C., Zhao J., Haffey W.D., Wyder M.A., Greis K.D., Setchell K.D, & Qualls J.E. (2022). L-arginine transfer from antigen presenting cells sustains CD4+ T cell viability and proliferation. Journal of immunology (Baltimore, Md. : 1950), 208(4), 793-798.

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Synthesis and Characterization of P(HEMA-co-OEGMA) Copolymer

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Dulbecco’s Modified Eagle Medium (DMEM) (D5030), sodium chloride (NaCl), sodium bicarbonate (NaHCO₃), potassium chloride (KCl), calcium chloride (CaCl₂), monosodium phosphate (NaH₂PO₄), copper(II) sulfate (CuSO₄), glucose, L-glutamine, L-lysine × HCl, L-isoleucine, L-leucine, L-tyrosine × 2Na, 2-hydroxyethyl methacrylate (HEMA, ≥99%), oligo(ethylene glycol) methyl ether methacrylate (OEGMA₃₀₀, M_n = 300 g/mol), and PNIPAM were purchased from Sigma-Aldrich (Germany) and used as received. The P(HEMA-co-OEGMA) with 90% mol of HEMA as well as homopolymers of OEGMA were synthesized at the Centre of Polymer and Carbon Materials of the Polish Academy of Sciences via atom transfer radical polymerization (ATRP) as described in [49 (link)]. The polymer characterization is summarized in Table 1.
The water used to form the solutions was purified using a commercial ion exchange system (Hydrolab, Poland) and filtered twice through a polytetrafluoroethylene (PTFE) filter (0.2 μm) (Merc Millipore, Burlington, MA, USA).

Otulakowski Ł, & Trzebicka B. (2023). Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts. Polymers, 15(17), 3587.

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Xeno-Free Expansion of OCT4-eGFP hESCs

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WA01 OCT4–eGFP Knock‐In hESC (WiCell Research Institute) were plated on a precoated xeno‐free vitronectin (VN XF, Primorigen Biosciences, Madison, WI, USA) 6‐well plate (coating concentration = 0.5 μg/cm²) and cultured in E8^TM medium (37°C, 5% CO₂, and 5% O₂). E8^TM medium was made by diluting E8^TM 50× supplement 1:50 with “arginine and lysine‐free” DMEM/F12 (Thermo Scientific, Rockford, IL, USA) supplemented with 398 μM l‐arginine HCl and 499 μM l‐lysine HCl (both from Sigma‐Aldrich, St. Louis, MO, USA). Splitting was performed every 4–5 days with 0.5 mM ethylenediaminetetraacetic acid in Dulbecco's PBS according to the manufacturer's protocol of culturing hESC in E8^TM medium.

Scheerlinck E., Van Steendam K., Daled S., Govaert E., Vossaert L., Meert P., Van Nieuwerburgh F., Van Soom A., Peelman L., De Sutter P., Heindryckx B., Dhaenens M, & Deforce D. (2016). Assessing the impact of minimizing arginine conversion in fully defined SILAC culture medium in human embryonic stem cells. Proteomics, 16(20), 2605-2614.

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SILAC Labeling of A549, Calu-1, and NCI-H1299 Cells

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Adherent human A549, Calu-1 and NCI-H1299 cells were cultured and passaged in high glucose DMEM (PAA Laboratories GmbH, Coelbe, Germany), supplemented with 10% FBS, 1% Penicillin/Streptomycin, and 1% l-glutamine. For SILAC-labeling, cells were grown in SILAC-DMEM (high glucose) (Thermo-Fisher Scientific, Langenselbold, Germany), supplemented with 10% dialyzed FBS (Invitrogen, Darmstadt, Germany), 1% Penicillin/Streptomycin, and 1% l-glutamine, containing a final concentration of 42 mg/l l-arginine HCl (Arg0), 73 mg/l l-lysine HCl (Lys0) and 1.33 mg/l l-proline for light labeling (Sigma-Aldrich, Taufenkirchen, Germany). Arg0 and Lys0 were replaced by l-arginine-¹³C₆¹⁴N₄ and l-lysine-²H₄ (Arg6, Lys4) for medium-heavy, or l-arginine-¹³C₆-¹⁵N₄ and l-lysine-¹³C₆¹⁵N₂ (Arg10, Lys8) for heavy labeled cells (Silantes, München, Germany). To gain full incorporation of labeled amino acids, cells were cultured for at least 5 cell doublings in the corresponding label. For harvesting, cells were washed 3 times with ice cold DPBS, collected with a cell scraper, centrifuged at 1000 × g for 5 min and cell pellets were stored at −80 °C for further use.

Becker A.C., Gannagé M., Giese S., Hu Z., Abou-Eid S., Roubaty C., Paul P., Bühler L., Gretzmeier C., Dumit V.I., Kaeser-Pebernard S., Schwemmle M., Münz C, & Dengjel J. (2018). Influenza A Virus Induces Autophagosomal Targeting of Ribosomal Proteins. Molecular & Cellular Proteomics : MCP, 17(10), 1909-1921.

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