Glutamax media

Manufactured by Thermo Fisher Scientific

Sourced in United States

Glutamax media is a cell culture supplement designed to support cell growth and proliferation. It is a chemically defined, animal-component-free, and serum-free media additive. Glutamax media provides a stable source of L-glutamine to support cellular metabolism and energy production.

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Lab products found in correlation

12 protocols using glutamax media

Transient Transfection of COS-1 and HEK293T Cells

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COS-1 and HEK293T cells (ATCC; Manassas, VA) were grown in 24-well plates or 100mm dishes containing Glutamax media (GIBCO; Gaithersburg, MD)/10% fetal bovine serum (FBS) at 37°C/5% CO₂. Plasmid DNA was transiently transfected using Optimem and Lipofectamine 2000 as per manufacturer’s instructions (Life Technologies). The morning following transfection, Optimem was replaced with Glutamax media/5% FBS and cells continued to grow for 5-24 hours. Cells were fixed with 4% paraformaldehyde for imaging studies or harvested in lysis buffer for Westerns. Experiments shown in Figures 3D-O were repeated ≥3 times with similar results.

Pathak G., Agostino M.J., Bishara K., Capell W.R., Fisher J.L., Hegde S., Ibrahim B.A., Pilarzyk K., Sabin C., Tuczkewycz T., Wilson S, & Kelly M.P. (2016). PDE11A negatively regulates lithium responsivity. Molecular psychiatry, 22(12), 1714-1724.

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Transient Transfection of COS-1 and HEK293T Cells

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Maintaining K562 and PLH Cell Lines

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The K562 cell line (ATCC CCL 243) and the lymphoblastoid EBV cell line PLH (IHW Number: 9047) were maintained in logarithmic growth in RPMI 1640 medium (Gibco^® GlutaMAX^™ media) with 10% fetal bovine serum (FBS) (Gibco^®). Cells were cultured at 37°C in a humidified chamber with 5% CO₂ in air, and passaged 1:10 twice a week.

Krzywinska E., Cornillon A., Allende-Vega N., Vo D.N., Rene C., Lu Z.Y., Pasero C., Olive D., Fegueux N., Ceballos P., Hicheri Y., Sobecki M., Rossi J.F., Cartron G, & Villalba M. (2016). CD45 Isoform Profile Identifies Natural Killer (NK) Subsets with Differential Activity. PLoS ONE, 11(4), e0150434.

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PBMC Expansion in G-Rex System

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As shown in Figure 1, thawed PBMC were seeded at 0.25-1.0E+06 cells/cm² at a DC:PBMC ratio of 1:10 in a G-Rex culture system (Wilson Wolf Manufacturing, New Brighton, MN). Fresh medium was composed of Roswell Park Memorial Institute GlutaMAX media (RPMI, Gibco Laboratories, NY, USA) supplemented with 10% (v/v) human AB serum (hSerAB) (Banc de Sang i Teixits, Barcelona, Spain). After 5 days of culture, the medium was completely replaced with RPMI+10% hSerAB supplemented with IL-2 120 U/mL (Miltenyi Biotec), IL-7 4,400 U/mL (R&D Systems, MN, USA), and IL-15 80 U/mL (Miltenyi Biotec). Moreover, monoclonal antibodies anti-CD3 (1 μg/mL) and anti-CD28 (2 μg/mL) were added (Biolegend, San Diego, California, USA). On days 7, 9, and 12, an aliquot of 100–300 μL was taken from each culture for cell counting and viability analysis, and fresh medium consisting of RPMI+10% hSerAB with the addition of IL-2 120 U/mL, IL-7 4,400 U/mL and IL-15 80 U/mL was added. Culture split was performed when cell density reached 8E+06 cells/cm².

Grau-Vorster M., López-Montañés M., Cantó E., Vives J., Oliver-Vila I., Barba P., Querol S, & Rudilla F. (2020). Characterization of a Cytomegalovirus-Specific T Lymphocyte Product Obtained Through a Rapid and Scalable Production Process for Use in Adoptive Immunotherapy. Frontiers in Immunology, 11, 271.

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Cell Culture in DMEM Glutamax Media

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Cells were grown in DMEM Glutamax media (Gibco, 10566016) supplemented with 10% fetal bovine serum at 37°C, 5% CO₂.

Chowdhury S., Wang J., Nuccio S.P., Mao H, & Di Antonio M. (2022). Short LNA-modified oligonucleotide probes as efficient disruptors of DNA G-quadruplexes. Nucleic Acids Research, 50(13), 7247-7259.

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Graphene Oxide Transfection Protocol

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Lipofectamine™ 2000 transfection reagent, Dulbecco’s modified Eagle minimum essential/Ham’s F-12 (DMEM/F12) plus Glutamax™ media, fetal bovine serum (FBS), sodium pyruvate, L-glutamine, penicillin G/streptomycin mix, RIPA buffer, and enzyme-free PBS-based cell dissociation buffer were purchased from Gibco (Carlsbad, CA, USA). Noble agar, Crystal Violet dye, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT); non-essential amino acids and protease inhibitor cocktail were acquired from Roche (Palo Alto, CA, USA). β-Mercaptoethanol and rotenone (Rot) were purchased to Sigma-Aldrich (St Louis, MO, USA). Partially reduced graphene oxide (PRGO) was prepared by Abalonyx AS (Oslo, Norway). Graphene oxide (GO) was prepared from natural graphite powder following the modification of the hummers method as follows. An aqueous slurry of GO was dried on a plastic substrate to prepare a GO film. This film was then heated slowly (1 °C/min) to 300 °C in open air to obtain the PRGO film.

Rodríguez-Losada N., de la Rosa J., Larriva M., Wendelbo R., Aguirre J.A., Castresana J.S, & Ballaz S.J. (2020). Overexpression of alpha-synuclein promotes both cell proliferation and cell toxicity in human SH-SY5Y neuroblastoma cells. Journal of Advanced Research, 23, 37-45.

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Swelling Behavior of GEL-LN Hydrogel

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The swelling behavior of the GEL-LN hydrogel was analyzed using a previously reported method^{36 (link)–38 (link)}. Hydrogel samples (n=10) were fabricated and measured for each condition. To measure the wet weight (W_e), the hydrogel constructs were weighed after 48 hours in high glucose DMEM GlutaMAX media (Gibco) at 37°C. The samples were dried at room temperature for 7 days to achieve constant weight, these values were recorded as the dry weight (W_d). The average mass of the glass coverslips was subtracted from both values. The equilibrium percentage of swelling was calculated as follows:

Percent Swelling = (W_{e} - W_{d}) / W_{d} x 100

Besser R.R., Bowles A.C., Alassaf A., Carbonero D., Claure I., Jones E., Reda J., Wubker L., Batchelor W., Ziebarth N., Silvera R., Khan A., Maciel R., Saporta M, & Agarwal A. (2020). Enzymatically Crosslinked Gelatin-Laminin Hydrogels for Applications in Neuromuscular Tissue Engineering. Biomaterials science, 8(2), 591-606.

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Neuronal Cell Culture Protocol

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Dulbecco's modified Eagle minimum essential/Ham's F-12 (DMEM/F12) plus Glutamax media, Neurobasal medium, minimum essential medium (MEM), B-27 supplement, fetal bovine serum (FBS), sodium pyruvate, l-glutamine, penicillin G/streptomycin mix, and enzyme-free PBS-based cell dissociation buffer were purchased from Gibco (Carlsbad, CA). Staurosporine [ready-made 1 mM solution in dimethyl sulfoxide (DMSO)], l-glutamic acid, d-glucose, uridine, sodium orthovanadate, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were acquired from Sigma-Aldrich (St. Louis, MO). Commercially available NNI insecticides were acetamiprid or ACE 200 g/kg soluble powder (SP) in talcum (non-toxic mineral clay) and imidacloprid or IMI 20 g/100 mL of concentrated suspension (CS) in water. They were only used certified brands of insecticides that fulfilled purity standards.

Ramirez-Cando L.J., Guzmán-Vallejos M.S., Aguayo L.G., Vera-Erazo F.D, & Ballaz S.J. (2023). Neurocytotoxicity of imidacloprid- and acetamiprid-based comercial insecticides over the differentiation of SH-SY5Y neuroblastoma cells. Heliyon, 9(5), e15840.

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Synthesizing and Utilizing JBNTs in hMSC Research

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JBNTs were synthesized by an effective approach published previously (Chen, Yu, & Chen, 2017 ). Human MSCs (hMSCs) (containing ≥750,000 cells/vial), Stem Cell Growth Medium BulletKit, and Tritcphalloidin were purchased from Lonza. Trypsin–EDTA solution (Gibco, 0.25%), phosphate buffered saline (PBS, Gibco), GlutaMAX media (Gibco), fetal bovine Solution (FBS, Gibco), Penicillin–Streptomycin (Gibco, 10,000 U/ml), and Triton X-100 (Invitrogen, 1.0%) were purchased from Thermo Fisher. Formalin and Ethanol (70% solution) were obtained from Fisher Scientific. Then, 12-well, 24-well, and 96-well clear round-bottom microplates were obtained from Corning. The catalog numbers of these plates are 353043, 353047, and 353,072, respectively. The 8-μm cell insert also was obtained from corning, the catalog number is 353182. The 8-well cell culture slides were purchased from Thermo Fisher, and the catalog number is 155409. FN (1.0 mg/ml) came from Gibco, and its catalog number is PHE0023.

Zhou L., Yau A., Yu H., Kuhn L., Guo W, & Chen Y. (2020). Self-assembled biomimetic Nano-Matrix for stem cell anchorage. Journal of biomedical materials research. Part A, 108(4), 984-991.

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Immortalized Fibroblasts for XP-C

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Wild type (AG10076) and XP-C (GM15983) immortalized patient derived-broblasts were purchased from Coriell Biorepository. XP-C broblasts possess a two-base pair shift mutation at codon 431 of the XPC gene. The cells were cultured in DMEM high glucose, GlutaMAX™ media (Gibco tM ) supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C in a 5% CO2 incubator.

Kobaisi F., Sulpice E., Barette C., Fayyad N., Fauvarque M., Badran B., Fayyad‐Kazan M., Fayyad‐Kazan H., Gidrol X., & Rachidi W. (2021). Isoconazole and Clemizole Hydrochloride Partially Reverse the Xeroderma Pigmentosum C Phenotype.

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