CBMC of 33 Australian newborns and 39 PNG newborns were studied for CD4 T cell frequencies using flow cytometry. Cells expressing high levels of CD4 were considered to be CD4 T cells, while cells expressing low CD4 levels were considered antigen‐presenting cells (APCs). Briefly, CBMC were resuspended in fluorescence‐activated cell sorting (FACS) buffer (1 × 106 cells/tube), incubated on ice with 50 μl of antibodies for CD4 (BD Biosciences, San Jose, CA, USA) (diluted 1 : 10), washed, and then fixed as per the manufacturer's instructions. Data were acquired using a four‐colour FACSCalibur (BD Biosciences) and results analysed using FlowJo software (Tree Star Inc., Stanford, CA, USA). Dead cells were excluded from analysis using a forward‐ versus side‐scatter gate.
Antibodies for cd4
Manufactured by BD
Sourced in United States
Antibodies for CD4 are laboratory reagents used to detect and measure the presence of CD4 protein on the surface of cells. CD4 is a marker commonly expressed on T helper cells, a type of lymphocyte that plays a crucial role in the immune response. These antibodies can be used in various immunological techniques, such as flow cytometry, to analyze and identify cell populations expressing CD4.
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Lab products found in correlation
Ack lysing buffer, by Thermo Fisher Scientific (1 mentions)
Phospho p38 p p38, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Sca 1, by BD (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Methocult gf m3434, by STEMCELL (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Total p38 t p38, by Cell Signaling Technology (1 mentions)
2 protocols using antibodies for cd4
1
Neonatal CD4 T Cell Analysis
2
Murine Hematopoietic Stem Cell Isolation
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CTX was purchased from Sigma–Aldrich (St. Louis, MO, USA) and antibodies for CD4, CD8, CD3, CD19, sca-1, Flk-2, c-kit, CD34, and IL-7R were purchased from BD Biosciences (Franklin Lakes, NJ, USA). Lineage antibody were purchased from BioLegend (San Diego, CA, USA). ACK lysing buffer was purchased from Gibco (Waltham, MA, USA). Methocult™ GF M3434 used for culturing bone marrow cells was purchased from STEMCELL Technologies Inc. (VN, Canada). Recombinant human granulocyte colony-stimulating factors (rhG-CSF) were purchased from R&D Systems (Minneapolis, MN, USA). Fetal bovine serum (FBS) was acquired from Welgene (Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea). Antibodies for Janus kinase 2 (JAK-2), p-JAK2, signal transducer and activator transcription 3 (STAT3), p-STAT3, suppressor of cytokines signaling 1 (SOCS1), suppressor of cytokines signaling 3 (SOCS3), c-Myc, β-actin, total c-Jun N-terminal kinase (T-JNK), phospho-c-Jun N-terminal kinase (p-JNK), total extracellular signal-regulated kinase (T-ERK), phospho-extracellular signal-regulated kinase (p-ERK), total-p38 (T-p38), and phospho-p38 (p-p38) were purchased from Cell Signaling Technology (Danver, MA, USA).
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