Blood samples were collected retroorbitally under anesthesia, and serum was obtained using a BD Microtainer. Serum growth hormone was measured using a rat/mouse growth hormone ELISA (EMD Millipore). Serum IGF-I was measured using mouse/rat IGF-I Quantikine ELISA kit (R&D Systems). Serum CTx-I was measured using a RatLaps ELISA kit (Nordic Bioscience Diagnostic A/S). Serum osteocalcin was measured using a mouse osteocalcin EIA kit (Biomedical Technologies). Plasma was obtained using a BD Microtainer.
Mouse rat igf 1 quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States, Canada
The Mouse/Rat IGF-1 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure mouse and rat insulin-like growth factor 1 (IGF-1) levels in cell culture supernates, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Mouse insulin elisa kit, by Mercodia (2 mentions)
Aprotinin, by Merck Group (2 mentions)
Glucose c2, by Fujifilm (2 mentions)
Microtainer, by BD (1 mentions)
Mouse osteocalcin eia kit, by Biomedical Technologies (1 mentions)
Creatine kinase activity assay kit, by Abcam (1 mentions)
Rat mouse gh elisa kit, by Merck Group (1 mentions)
Rpmi 1640 glutamax, by Thermo Fisher Scientific (1 mentions)
Glucose colorimetric assay kit, by Cayman Chemical (1 mentions)
Rat mouse insulin elisa kit, by Merck Group (1 mentions)
Assaymax corticosterone elisa kit, by Assaypro (1 mentions)
Glucose cii test wako, by Fujifilm (1 mentions)
Nefa c test wako, by Fujifilm (1 mentions)
Corticosterone enzyme immunoassay kit, by Arbor Assays (1 mentions)
Mouse insulin elisa kit, by Crystal Chem (1 mentions)
Breeze2, by Bayer (1 mentions)
Fluostar omega, by BMG Labtech (1 mentions)
Rat il 1β il 1f2 quantikine elisa kit, by R&D Systems (1 mentions)
Bio plex magpix multiplex reader, by Bio-Rad (1 mentions)
Vitros analyzer, by Ortho Clinical Diagnostics (1 mentions)
26 protocols using mouse rat igf 1 quantikine elisa kit
1
Blood Biomarkers Measurement Protocol
2
Quantifying IGF-I and CK in Mice
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The levels of IGF-I and CK in mouse serum were quantified with a Mouse/Rat IGF-I Quantikine ELISA kit (MG100, R&D systems) and a Creatine Kinase Activity Assay Kit (Clorimetric; ab155901, Abcam), respectively, according to the manufacturers’ instructions.
3
Fasting Induces Hormonal Changes
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Blood was collected from mice 6 hr after fasting. Serum insulin and GH levels were measured using the Mercodia mouse insulin ELISA Kit (Mercodia AB, Uppsala, Sweden) and the rat/mouse GH ELISA Kit (EMD Millipore, ON, Canada), respectively. IGF-I levels in the serum or in protein extracts were analyzed using the Mouse/Rat IGF-I Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA).
4
Plasma Analyses of EphA4 Mice
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Plasma samples were collected when the two genotypic mice of EphA4‐KO and EphA4‐WT were killed as mentioned above. The plasma samples were stored at −80°C for use. Plasma G‐CSF and IGF1 concentration was examined by ELISA assay (Mouse ELISA Kit ab100684‐G‐CSF abcam and Mouse/Rat IGF‐I Quantikine ELISA Kit R&D Systems).
5
Quantifying Muscle and Serum IGF-I
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Serum IGF-I concentrations were measured using a Mouse/Rat IGF-I Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. For the measurement of muscle IGF-I concentrations, 50 mg of gastrocnemius muscle were homogenized with 1 ml of lysis buffer containing 50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM NaF, 1 mM Na3VO4, and 1 mM PMSF. After incubation on ice for 30 min, the samples were centrifuged at 15,000 rpm for 10 min and subsequently for 30 min at 4 °C. IGF-I concentrations of the supernatants were measured and the results were adjusted by the protein concentrations of the supernatants.
6
Measuring IGF-1 Secretion in Dunning G Cells
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Dunning G cells (1.5 × 105) were seeded in six well plates in RPMI 1640 + GlutaMAX (Gibco, Life Technologies, Grand Island, NY, US) supplemented with 10% FBS and 250 nM dexamethasone, and 24 h later medium was replaced with phenol-free αMEM (Gibco) supplemented with 0.1% albumin. After another 24 h, medium was replaced by fresh medium, with or without the addition of 10 nM DHT. Levels of IGF-1 released from Dunning G cells was measured in the culture medium after 72 h using the Mouse/Rat IGF-1 Quantikine ELISA kit (MG100, R&D Systems) according to the manufacturer’s instructions.
7
Glucose, Insulin, and FFA Quantification
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Blood glucose level was determined by Glucose Colorimetric Assay kit (Cayman Chemical, Ann Arbor, MI, USA) and collected during GTT. Because of the higher glucose concentration in diabetic animals, samples from STZ and STZ + Hex groups were diluted 1:20, while 1:5 dilution was applied to control and control + Hex groups. Circulating levels of IGF-1, insulin, and FFAs were determined by Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA), Mouse/Rat Insulin ELISA kit (EMD Millipore, St. Charles, MI, USA), and nonesterified fatty acids (NEFA-C) Assay (Wako, Osaka, Japan), respectively. All procedures were performed according to the manufacturer’s instructions.
8
Plasma Biomarker Quantification Protocol
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Blood collected in EDTA-coated tubes was immediately separated by centrifugation for 10 min at 5,800 × g. Plasma was collected and stored at −80 °C. Plasma albumin, glucose, insulin, alanine, β-hydroxybutyrate, free fatty acid (FFA), glucocorticoid, and IGF-1 values were measured using A/G B-Test Wako (Wako Pure Chemical Industries, Osaka, Japan), Glucose CII-test Wako (Wako), Mouse Insulin ELISA kits (Morinaga Institute of Biological Science, Kanagawa, Japan), Alanine Colorimetric/Fluorometric Assay Kits (BioVision, Milpitas, CA, USA), β-Hydroxybutyrate (β-HB) Colorimetric Assay Kits (BioVision), NEFA C-test Wako (Wako), AssayMax corticosterone ELISA kits (AssayPro, St. Charles, MO, USA) and Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA) respectively.
9
Blood and Brain Tissue Collection for Metabolic Analysis
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Blood samples were collected from the retroorbital vein under isoflurane anesthesia and immediately transferred to tubes containing ethylenediaminetetraacetic acid (EDTA; 10 μl of 0.2 M EDTA/tube) and aprotinin (0.1 mg/tube, Merck KGaA). The blood samples were centrifuged 3000×g for 5 min at 4 °C, and the plasma was separated and stored at −80 °C until assayed. After blood collection, the mice were killed by decapitation. The brain was rapidly removed from the skull and placed on an ice-cooled paraffin plate for dissection of the hippocampus as previously described (Nakao et al., 1986 (link)). The hippocampus was immediately frozen in liquid nitrogen and stored at −80 °C until analyzed. Glucose (Glucose C2; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), insulin (Morinaga Ultra Sensitive Mouse/Rat Insulin ELISA Kit; Morinaga Institute of Biological Science, Yokohama, Japan), corticosterone (Corticosterone Enzyme Immunoassay Kit; Arbor Assays, Ann Arbor, MI, USA) and IGF-1 (Mouse/Rat IGF-1 Quantikine ELISA Kit; R&D Systems, Inc., Minneapolis, MN, USA) were measured using commercially available kits.
10
Serum IGF1 Quantification Protocol
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At sacrificing, serum was collected from clotting blood. Serum IGF1 was measured by mouse/rat IGF1 Quantikine ELISA kit (R&D Systems, Inc) according to the manufacturer’s instructions.
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