Metabolite profiling analysis of the parental seedlings was performed as described previously (Dan et al., 2020 (link)). Briefly, untargeted metabolite profiles of 15-d-old seedlings were collected with a 1290 Infinity liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA), Agilent quadrupole time-of-flight mass spectrometer (Agilent 6550 iFunnel QTOF; Agilent Technologies, Santa Clara, CA, USA), and Triple TOF 6600 mass spectrometer (AB SCIEX, Foster City, CA, USA). The metabolites were annotated using an in-house standard spectral library, and the lipids were annotated through matching with an in-house tandem mass spectrometry (MS/MS) spectral library. Data reliability was checked using a quality control sample, and the metabolite levels of a total of 3,746 detected analytes, among which 114 metabolites were annotated using the in-house spectral libraries, were normalized (sum, log, and none) for the statistical analyses.
1290 infinity liquid chromatography system
Manufactured by Agilent Technologies
Sourced in United States
The 1290 Infinity liquid chromatography system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It is capable of delivering mobile phases at high pressure and accurately controlling flow rates, enabling efficient separation and analysis of complex sample mixtures.
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2 protocols using 1290 infinity liquid chromatography system
1
Untargeted Metabolite Profiling of Seedlings
2
Chromatographic Separation and Analysis of Analytes
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The chromatographic separation of analytes was achieved on an Agilent Poroshell 120 Bonus-RP LC column (2.1 mm × 100 mm 2.7 μm particle size) using the Agilent 1290 infinity liquid chromatography system coupled to the Agilent 6490 triple quadrupole mass spectrometer. A gradient elution was performed at a flow rate of 0.18 mL/min with two mobile phases. Mobile phase A was composed of water containing 0.2% (v/v) formic acid and mobile phase B comprised of acetonitrile with 0.1% (v/v) formic acid. The gradient elution are as follows: 100% A (0 min) ➔ 100% A (hold 2 min) ➔ 95% B (linear increase in 0.5 min) ➔ 95% B (hold 2.5 min) ➔ 100% A (linear decrease in 0.25 min) ➔ 100% A (hold 3 min).
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