After being treated as indicated, the cardiomyocytes cultured on glass coverslips in 24-well plates were washed with PBS for three times, fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, permeabilized with 0.1% Triton X-100 for 10 min, and then blocked with 5% BSA for 1 h. Then, cardiomyocytes were incubated overnight at 4°C with monoclonal mouse anti-rat SERCA2a antibody (1: 100; Abcam, Cambridge, USA). After being washed with PBS for three times, cardiomyocytes were incubated with goat anti-mouse polyclonal IgG (1: 400; Abcam, Cambridge, USA) at room temperature in the dark for 2 h. For nuclear counterstaining, cardiomyocytes were incubated with 4,6-diamidino-2-phenylidone (DAPI; Sigma, USA) for 5 min. At last, the immunofluorescence images were obtained by inverted fluorescence microscope (Olympus, Tokyo, Japan). The myocardium was also treated according to instruction for immunofluorescence staining.
Monoclonal mouse anti rat serca2a antibody
Manufactured by Abcam
Sourced in United States
The Monoclonal mouse anti-rat SERCA2a antibody is a laboratory reagent used for the detection and study of the SERCA2a protein, which is a calcium pump found in the sarcoplasmic reticulum of muscle cells. This antibody can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to identify and quantify the SERCA2a protein in biological samples.
Automatically generated - may contain errors
2 protocols using monoclonal mouse anti rat serca2a antibody
1
Immunofluorescence Analysis of SERCA2a in Cardiomyocytes
2
Immunohistochemical Analysis of SERCA2a
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After being treated as above, the heart sections were fixed with 4%
paraformaldehyde in PBS for 15 min at room temperature, permeabilized with 0.1%
Triton X-100 for 10 min, and then blocked with 5% BSA for 1 h. Then, samples
were incubated overnight at 4ºC with monoclonal mouse anti-rat SERCA2a
antibody (1:100;Abcam, Cambridge, USA). After being washed with PBS for three
times, samples were incubated with goat anti-mouse polyclonal IgG (1:400;
Abcam,Cambridge, USA) at room temperature in the dark for 2 h. For nuclear
counterstaining, samples were incubated with 4’, 6-diamidino-2-phenylidone
(DAPI; Sigma, USA) for 5 min. Finally, the immunofluorescence images were
obtained by inverted fluorescence microscope (Olympus, Tokyo, Japan).
paraformaldehyde in PBS for 15 min at room temperature, permeabilized with 0.1%
Triton X-100 for 10 min, and then blocked with 5% BSA for 1 h. Then, samples
were incubated overnight at 4ºC with monoclonal mouse anti-rat SERCA2a
antibody (1:100;Abcam, Cambridge, USA). After being washed with PBS for three
times, samples were incubated with goat anti-mouse polyclonal IgG (1:400;
Abcam,Cambridge, USA) at room temperature in the dark for 2 h. For nuclear
counterstaining, samples were incubated with 4’, 6-diamidino-2-phenylidone
(DAPI; Sigma, USA) for 5 min. Finally, the immunofluorescence images were
obtained by inverted fluorescence microscope (Olympus, Tokyo, Japan).
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