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Taqman assaysfrom

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TaqMan assays are a type of real-time PCR (polymerase chain reaction) assays developed by Thermo Fisher Scientific. They are designed for the detection and quantification of specific DNA or RNA sequences. TaqMan assays utilize a fluorescent probe that hybridizes to the target sequence, allowing for the real-time monitoring of the amplification process.

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Lab products found in correlation

Abi 7500 real time thermal cycler, by Thermo Fisher Scientific (1 mentions) Taqman reverse transcription reagents kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using taqman assaysfrom

1

Real-Time PCR Gene Expression Analysis

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Gene expression was quantitated by real-time PCR, using TaqMan assays
from Applied Biosystems, an ABI 7500 real-time thermal cycler, and ABI software
(Life Technologies).
Hayakawa K., Formica A.M., Colombo M.J., Shinton S.A., Brill-Dashoff J., Morse HC I.I.I., Li Y.S, & Hardy R.R. (2016). Loss of a chromosomal region with synteny to human 13q14 occurs in mouse chronic lymphocytic leukemia that originates from early-generated B1 B cells. Leukemia, 30(7), 1510-1519.
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2

Assessing NK Cell Gene Expression

Cited 1 time
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Total RNA was extracted as described previously (Jeffery, et al. 2012 (link)). cDNA was prepared by reverse transcription Taqman Reverse Transcription Reagents Kit (Applied Biosystems, Roche, Nersey, USA) as per manufacturer’s instructions. qRT-PCR was performed on an ABI 7500 qPCR machine using Taqman assays from Applied Biosystems for VDR (Hs00172113_m1) and multiplexed with VIC labelled 18S rRNA (4319413E) (Applied Biosystems, Roche, Nersey, USA). Paired uNK and pNK gene expression following 24-hour culture in the presence and absence of CK-stimulation with and without 1,25(OH)2D3 co-treatment was measured. Relative expression compared to paired unstimulated pNK was assessed using fold-change calculated by the 2−ΔΔCt method. All statistical analyses were carried out using Sigmaplot 9.0 software (Systat Inc., San Jose, CA, USA). Experimental means were compared statistically using one-way ANOVA, with the Holm-Sidak method used as a post hoc multiple-comparison procedure. Statistical analyses for RT-qPCR analyses were carried out using raw ΔCt values.
Tamblyn J., Jeffery L., Susarla R., Lissauer D., Coort S., Garcia A.M., Knoblich K., Fletcher A., Bulmer J., Kilby M, & Hewison M. (2019). Transcriptomic analysis of vitamin D responses in uterine and peripheral NK cells. Reproduction (Cambridge, England), 158(2), 211-221.
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