Total caveolin 1

Western blot analysis of either lung homogenates or whole cell lysates was carried out as previously described [26 (link),27 (link)]. Antibodies against pY419 Src and total Src were purchased from Cell Signaling Technologies (Danvers, MA), for pY512 ICAM-1 and total ICAM-1 from Santa Cruz Biotechnology (Santa Cruz, CA), and for pY14 caveolin-1, total caveolin-1, and β-actin from BD Biosciences (Franklin Lakes, NJ).

All reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless stated otherwise. NS1643 was from Alamo Laboratories Inc. (San Antonio, TX, USA). Calpeptin was purchased from Tocris Bioscience (Minneapolis, MN, USA). PTP1B inhibitor was from Cayman Chemical (Ann Arbor, MI, USA). Phospho-Y14, total Caveolin-1, β-catenin, and caspase-3 antibodies were from BD Biosciences (San Jose, CA, USA). Phospho-Y416, Y527, and total Src were from Cell Signaling Technology (Danvers, MA, USA). PTP1B, desmoplakin, desmoglein, and plakophilin monoclonal Abs, normal mouse IgG, and protein A/G agarose beads were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). DAPI and all fluorescently labeled secondary antibodies were purchased from Molecular Probes (ThermoFisher Scientific, Waltham, MA, USA). Active β-catenin monoclonal antibody was purchased from MilliporeSigma (Burlington, MA, USA). HRP-conjugated goat-anti-mouse and goat-anti-rabbit secondary antibodies were from KPL (Gaithersburg, MD, USA). Lipofectamine 2000, ECL Super Signal kit, and Crosslink magnetic IP/Co-IP kit were from ThermoFisher Scientific (Waltham, MA, USA).